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Vol. 21, No. 3, 2008
Issue release date: June 2008
Skin Pharmacol Physiol 2008;21:166–180

Assessment of Quantum Dot Penetration into Intact, Tape-Stripped, Abraded and Flexed Rat Skin

Zhang L.W. · Monteiro-Riviere N.A.
Center for Chemical Toxicology Research and Pharmacokinetics, North Carolina State University, Raleigh, N.C., USA

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Quantum dot (QD) nanoparticles have received attention due to their fluorescent characteristics and potential use in medical applications. Skin penetration is one of the major routes of exposure for nanoparticles to gain access to a biological system. QD655 and QD565 coated with carboxylic acid were studied for 8 and 24 h in flow-through diffusion cells with flexed, tape-stripped and abraded rat skin to determine if these mechanical actions could perturb the barrier and affect penetration. Nonflexed skin did not show QD penetration at 8 or 24 h. Flexed skin showed an increase in QD on the surface of skin but no penetration at 8 and 24 h. Tape-stripped skin depicted QD only on the surface of the viable epidermis. QD655 penetrated into the viable dermal layers of abraded skin at both 8 and 24 h, while QD565 was present only at 24 h. QD were not detected in the perfusate by fluorescence and inductively coupled plasma-optical emission spectroscopy analysis for cadmium at any time point. These results indicate that the rat skin penetration of QD655 and QD565 is primarily limited to the uppermost stratum corneum layers of intact skin. Barrier perturbation by tape stripping did not cause penetration, but abrasion allowed QD to penetrate deeper into the dermal layers.

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  1. Larson DR, Zipfel WR, Williams RM, Clark SW, Bruchez MP, Wise FW, Webb WW: Free full text water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science 2003;5624:1434–1436.

    External Resources

  2. Wang J, Yong WH, Sun Y, Vernier PT, Koeffler HP, Gundersen MA, Marcu L: Receptor-targeted quantum dots: fluorescent probes for brain tumor diagnosis. J Biomed Opt 2007;12:044021.

    External Resources

  3. Cui B, Wu C, Chen L, Ramirez A, Bearer EL, Li WP, Mobley WC, Chu S: One at a time, live tracking of NGF axonal transport using quantum dots. Proc Natl Acad Sci USA 2007;34:13666–13671.

    External Resources

  4. Derfus AM, Chan WCW, Bhatia SN: Probing the cytotoxicity of semiconductor quantum dots. Nano Lett 2004;4:11–18.
  5. Chang E, Thekkek N, Yu WW, Colvin VL, Drezek R: Evaluation of quantum dot cytotoxicity based on intracellular uptake. Small 2006;12:1412–1417.

    External Resources

  6. Gopee NV, Roberts DW, Webb P, Cozart CR, Siitonen PH, Warbritton AR, Yu WW, Colvin VL, Walker NJ, Howard PC: Migration of intradermally injected quantum dots to sentinel organs in mice. Toxicol Sci 2007;98:249–257.
  7. Lee HA, Imran M, Monteiro-Riviere NA, Colvin VL, Yu WW, Riviere JE: Biodistribution of quantum dot nanoparticles in perfused skin: evidence of coating dependency and periodicity in arterial extraction. Nano Lett 2007;9:2865–2870.

    External Resources

  8. Yang RS, Chang LW, Wu JP, Tsai MH, Wang HJ, Kuo YC, Yeh TK, Yang CS, Lin P: Persistent tissue kinetics and redistribution of nanoparticles, quantum dot 705, in mice: ICP-MS quantitative assessment. Environ Health Perspect 2007;115:1339–1343.
  9. Soo Choi H, Liu W, Misra P, Tanaka E, Zimmer JP, Itty Ipe B, Bawendi MG, Frangioni JV: Renal clearance of quantum dots. Nat Biotechnol 2007;25:1165–1170.
  10. Gamer AO, Leibold E, van Ravenzwaay B: The in vitro absorption of microfine zinc oxide and titanium dioxide through porcine skin. Toxicol In Vitro 2006;20:301–307.
  11. Cross SE, Innes B, Roberts MS, Tsuzuki T, Robertson TA, McCormick P: Human skin penetration of sunscreen nanoparticles: in-vitro assessment of a novel micronized zinc oxide formulation. Skin Pharmacol Physiol 2007;20:148–154.
  12. Baroli B, Ennas MG, Loffredo F, Isola M, Pinna R, López-Quintela MA: Penetration of metallic nanoparticles in human full-thickness skin. J Invest Dermatol 2007;127:1701–1712.
  13. Shim J, Seok KH, Park WS, Han SH, Kim J, Chang IS: Transdermal delivery of minoxidil with block copolymer nanoparticles. J Control Release 2004;97:477–484.
  14. Ryman-Rasmussen J, Riviere JE, Monteiro-Riviere NA: Penetration of intact skin by quantum dots with diverse physicochemical properties. Toxicol Sci 2006;91:159–165.
  15. Schaefer H, Redelmeier TE: Skin barrier; in Schaefer H, Redelmeier TE (eds): Skin Barrier: Principles of Percutaneous Absorption. Basel, Karger, 1996, pp 146–148.
  16. Treffel P, Gabard B: Skin penetration and sun protection factor of ultra-violet filters from two vehicles. Pharm Res 1996;13:770–774.
  17. Hostýnek JJ, Dreher F, Pelosi A, Anigbogu A, Maibach HI: Human stratum corneum penetration by nickel: in vivo study of depth distribution after occlusive application of the metal as powder. Acta Derm Venereol Suppl 2001;212:5–10.
  18. Fent KW, Jayaraj K, Gold A, Ball LM, Nylander-French LA: Tape-strip sampling for measuring dermal exposure to 1,6-hexamethylene diisocyanate. Scand J Work Environ Health 2006;32:225–240.
  19. Orentreich N, Orentreich DS: Dermabrasion. Dermatol Clin 1995;15:313–327.
  20. Grimes PE: Microdermabrasion. Dermatol Surg 2005;31:1160–1165.
  21. Lee WR, Shen SC, Wang KS, Hu CH, Fang JY: Lasers and microdermabrasion enhance and control topical delivery of vitamin C. J Invest Dermatol 2003;121:1118–1125.
  22. Tinkle SS, Antonini JM, Rich BA, Roberts JR, Salmen R, DePree K, Adkins EJ: Skin as a route of exposure and sensitization in chronic beryllium disease. Environ Health Perspect 2003;111:1202–1208.
  23. Rouse JG, Yang J, Ryman-Rasmussen JP, Barron AR, Monteiro-Riviere NA: Effects of mechanical flexion on the penetration of fullerene amino acid-derivatized peptide nanoparticles through skin. Nano Lett 2007;7:155–160.
  24. Monteiro-Riviere NA: Specialized technique: the isolated perfused porcine skin flap (IPPSF); in Kemppainen BW, Reifenrath WG (eds): Methods for Skin Absorption. Boca Raton, CRC Press, 1990, pp 175–189.
  25. Lademann J, Ilgevicius A, Zurbau O, Liess HD, Schanzer S, Weigmann HJ, Antoniou C, Pelchrzim RV, Sterry W: Penetration studies of topically applied substances: optical determination of the amount of stratum corneum removed by tape stripping. J Biomed Opt 2006;11:054026.
  26. Teichmann A, Jacobi U, Ossadnik M, Richter H, Koch S, Sterry W, Lademann J: Differential stripping: determination of the amount of topically applied substances penetrated into the hair follicles. J Invest Dermatol 2005;125:264–269.
  27. Choi MJ, Zhai H, Kim JH, Maibach HI: Tape stripping method versus stratum corneum; in Zhai H, Wilhelm K, Maibach HI (eds): Dermatotoxicology. Boca Raton, CRC Press, 2007, pp 327–337.
  28. Lee WR, Tsai RY, Fang CL, Liu CJ, Hu CH, Fang JY: Microdermabrasion as a novel tool to enhance drug delivery via the skin: an animal study. Dermatol Surg 2006;32:1013–1022.
  29. Monteiro-Riviere NA, Bristol DG, Manning TO, Rogers RA, Riviere JE: Interspecies and interregional analysis of the comparative histologic thickness and laser Doppler blood flow measurements at five cutaneous sites in nine species. J Invest Dermatol 1990;95:582–586.
  30. Barry BW: Skin transport; in Barry BW (ed): Dermatological Formulations: Percutaneous Absorption. New York, Dekker, 1983, pp 95–116.
  31. Bronaugh RL, Stewart RF, Congdon ER: Methods for in vitro percutaneous absorption studies. 2. Animal models for human skin. Toxicol Appl Pharmacol 1982;62:481–488.
  32. Zhang LW, Yu WW, Colvin VL, Monteiro-Riviere NA: Biological interactions of quantum dot nanoparticles in skin and in human epidermal keratinocytes. Toxicol Appl Pharmacol 2008;228:200–211.
  33. Kraeling ME, Gopee NV, Roberts DW, Ogunsola OA, Walker NJ, Yu WW, Colvin VL, Howard PC, Bronaugh RL: Evaluation of in vitro penetration of quantum dot nanoparticles into human skin. Toxicologist 2007;96:196.
  34. Monteiro-Riviere NA: Comparative anatomy, physiology, and biochemistry of mammalian skin; in Hobson DW (ed): Dermal and Ocular Toxicology Fundamentals and Methods. Boca Raton, CRC Press, 1991, pp 3–71.
  35. Monteiro-Riviere NA: Integument; in Dellmann HD, Eurell JA (eds): Textbook of Veterinary Histology. Baltimore, Williams & Wilkins, 1998, pp 303–332.
  36. Monteiro-Riviere NA: Anatomical factors that affect barrier function; in Zhai H, Wilhelm KP, Maibach HI (eds): Dermatotoxicology. New York, CRC Press, 2008, pp 39–50.
  37. Alvarez-Roman R, Naik A, Kalia YN, Guy RH, Fessi H: Skin penetration and distribution of polymeric nanoparticles. J Control Release 2004;99:53–62.
  38. Lademann J, Weigmann H, Rickmeyer C, Barthelmes H, Schaefer H, Mueller G, Sterry W: Penetration of titanium dioxide microparticles in a sunscreen formulation into the horny layer and the follicular orifice. Skin Pharmacol Appl Skin Physiol 1999;12:247–256.

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