Assignment of the human PAX4 gene to chromosome band 7q32 by fluorescence in situ hybridizationTamura T. · Izumikawa Y. · Kishino T. · Soejima H. · Jinno Y. · Niikawa N.
aDepartment of Human Genetics, Nagasaki University School of Medicine, Nagasaki; bDepartment of Child Development, Kumamoto University School of Medicine, Kumamoto; cDepartment of Pediatrics, University of the Ryukyus School of Medicine, Okinawa (Japan)
Of the nine known members of a human paired box-containing gene family (Pax), only PAX4 has not been precisely localized. We screened a cosmid library of human genomic DNA using polymerase chain reaction products for PAX4 as a probe and isolated three positive cosmid clones. Sequence analysis revealed that at least two of them had exon-like sequences and showed extensive homology to Pax-4 in the mouse. These two cosmid clones were mapped to human chromosome band 7q32 by fluorescence in situ hybridization.
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