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Table of Contents
Vol. 72, No. 4, 1996
Issue release date: 1996
Cytogenet Cell Genet 1996;72:294–296
(DOI:10.1159/000134206)

Assignment of the human α-tropomyosin gene TPM4 to band 19p13.1 by fluorescence in situ hybridization

Wilton SD. · Lim L. · Dorosz S.D. · Gunn H.C. · Eyre H.J. · Callen D.F. · Laing N.G.
aAustralian Neuromuscular Research Institute, QEII Medical Centre, Nedlands, Western Australia, andaCentre for Medical Genetics, Department of Cytogenetics and Molecular Genetics, Women’s and Children’s Hospital, Adelaide, South Australia (Australia)

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Abstract

Sequence-tagged sites (STSs) were developed for the human α-tropomyosin gene TPM4. One STS was used to amplify DNA from somatic cell hybrids to localize TPM4 to chromosome 19. The other, a product from a long-range PCR, was used directly as a probe to refine the localization of TPM4 to 19p13.1 by fluorescence in situ hybridization to metaphase chromosome spreads.



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