Assignment of the human α-tropomyosin gene TPM4 to band 19p13.1 by fluorescence in situ hybridizationWilton SD. · Lim L. · Dorosz S.D. · Gunn H.C. · Eyre H.J. · Callen D.F. · Laing N.G.
aAustralian Neuromuscular Research Institute, QEII Medical Centre, Nedlands, Western Australia, andaCentre for Medical Genetics, Department of Cytogenetics and Molecular Genetics, Women’s and Children’s Hospital, Adelaide, South Australia (Australia)
Sequence-tagged sites (STSs) were developed for the human α-tropomyosin gene TPM4. One STS was used to amplify DNA from somatic cell hybrids to localize TPM4 to chromosome 19. The other, a product from a long-range PCR, was used directly as a probe to refine the localization of TPM4 to 19p13.1 by fluorescence in situ hybridization to metaphase chromosome spreads.
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