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Vol. 74, No. 4, 1996
Issue release date: 1996
Cytogenet Cell Genet 1996;74:252–254

Molecular cloning and mapping of a human cDNA (SC5DL) encoding a protein homologous to fungal sterol-C5-desaturase

Matsushima M. · Inazawa J. · Takahashi E. · Suzumori K. · Nakamura Y.
aLaboratory of Molecular Medicine, Institute of Medical Science, University of Tokyo, Tokyo; bDepartment of Gynecology and Obstetrics, Nagoya City University, Nagoya; cDepartment of Hygiene, Kyoto Prefectural University of Medicine, Kyoto; dOtsuka GEN Research Institute, Otsuka Pharmaceutical Co., Ltd., Tokushima (Japan)

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We have isolated a human cDNA clone homologous to fungal ERG3, a gene encoding sterol C-5 desaturase. The full nucleotide sequence of this human cDNA revealed a 708-bp open reading frame that encodes a 236-amino-acid polypeptide. The gene was expressed in all normal human tissues examined. We determined its location to chromosome 11q23.3 by fluorescence in situ hybridization.

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