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Vol. 79, No. 1-2, 1997
Issue release date: 1997
Section title: Gene Mapping, Cloning, and Sequencing
Cytogenet Cell Genet 1997;79:132–138
(DOI:10.1159/000134700)

Cytochrome b in human complex II (succinate-ubiquinone oxidoreductase): cDNA cloning of the components in liver mitochondria and chromosome assignment of the genes for the large (SDHC) and small (SDHD) subunits to 1q21 and 11q23

Hirawake H. · Taniwaki M. · Tamura A. · Kojima S. · Kita K.
aDepartment of Parasitology, The Institute of Medical Science, The University of Tokyo, Tokyo; bThe Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto (Japan)

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Article / Publication Details

First-Page Preview
Abstract of Gene Mapping, Cloning, and Sequencing

Accepted: 2/13/1997
Published online: 5/20/2008

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

Complex II (succinate-ubiquinone oxidoreductase) is an important enzyme complex in both the tricarboxylic acid cycle and the aerobic respiratory chains of mitochondria in eukaryotic cells and prokaryotic organisms. In this study, the amino acid sequences of the large (cybL) and small (cybS) subunits of cytochrome b in human liver complex II were deduced from cDNAs isolated by homology probing with mixed primers for the polymerase chain reaction. The mature cybL and cybS contain 140 and 103 amino acids, respectively, and show little similarity to the amino acid sequences of the subunits from other species in contrast to the highly conserved features of the flavoprotein (Fp) subunit and iron-sulfur protein (Ip) subunit. From hydrophobicity analysis, both cybL and cybS appear to have three transmembrane segments, indicating their role as membrane-anchors for the enzyme complex. Histidine residues, which are possible heme axial ligands in cytochrome b of complex II, were found in the second transmembrane segment of each subunit. The genes for cybL (SDHC) and cybS (SDHD) were mapped to chromosome 1q21 and 11q23, respectively by fluorescent in situ hybridization (FISH).


Article / Publication Details

First-Page Preview
Abstract of Gene Mapping, Cloning, and Sequencing

Accepted: 2/13/1997
Published online: 5/20/2008

Number of Print Pages: 7
Number of Figures: 0
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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