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Vol. 52, No. 3, 2008
Issue release date: July 2008
Ann Nutr Metab 2008;52:241–249

Effects of Simultaneous Intakes of Fish Oil and Green Tea Extracts on Plasma, Glucose, Insulin, C-Peptide, and Adiponectin and on Liver Lipid Concentrations in Mice Fed Low- and High-Fat Diets

Shirai N. · Suzuki H.
aNational Food Research Institute, Kannondai, Tsukuba, and bKagawa Nutrition University, Chiyoda, Sakado, Japan

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Aims: The purpose of this study was to clarify the effects of a simultaneous intake of fish oil and green tea extracts (GTE) on plasma lipids, glucose (Glu), insulin, C-peptide, and adiponectin concentrations and on liver lipids in mice fed low- and high-fat diets. Methods: Male mice (5 months old) were fed low-fat (5%) or high-fat (20%) diets containing 0% (5 or 20% lard), 1% (4 or 19% lard) or 5% (0 or 15% lard) fish oil with or without 1% GTE for 3 months. Results: Single or combined intakes of fish oil and GTE intakes significantly lowered plasma and liver total cholesterol (T-chol) and triacylglycerol (TG) concentrations in mice on high-fat diets. However, a positive effect of GTE alone was not observed in the plasma T-chol and TG concentrations of mice on low-fat diets. Plasma Glu concentrations were significantly lowered by dietary fish oil in mice on the low- and high-fat diets. A tendency of GTE intake to decrease plasma Glu in mice on both the low- and high-fat diets was not significant. An intake of GTE only minimally influenced plasma insulin, C-peptide, and adiponectin concentrations, but fish oil supplementation increased the adiponectin concentration in mice on the low- and high-fat diets. GTE addition to the diets had no adverse effects on reductions by fish oil. Conclusion: These results suggest that a simultaneous intake of fish oil and GTE can improve plasma and liver lipid and plasma Glu profiles in mice fed a high-fat diet without any negative interactions of fish oil and GTE.

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  1. Yamada T, Strong JP, Ishii T, Ueno T, Koyama M, Wagayama H, Shimizu A, Sakai T, Malcom GT, Guzman MA: Atherosclerosis and ω–3 fatty acids in the populations of a fishing village and a farming village in Japan. Atherosclerosis 2000;153:469–481.
  2. Rissanen T, Voutilainen S, Nyyssonen K, Lakka TA, Salonen JT: Fish oil- derived fatty acids, docosahexaenoic acid and docosapentaenoic acid, and the risk of acute coronary events. The Kuopio Ischaemic Heart Disease Risk Factor Study. Circulation 2000;102:2677–2679.
  3. Imai K, Suga K, Nakachi K: Cancer-preventive effects of drinking green tea among a Japanese population. Prev Med 1997;26:769–775.
  4. Imai K, Nakachi K: Cross-sectional study of effects of drinking green tea on cardiovascular and liver diseases. BMJ 1995;310:693–696.
  5. Shimazu T, Kuriyama S, Hozawa A, Ohmori K, Sato Y, Nakaya N, Nishino Y, Tsubono Y, Tsuji I: Dietary patterns and cardiovascular disease mortality in Japan: a prospective cohort study. Int J Epidemiol 2007;36:600–609.
  6. Von Schacky C: A review of omega–3 ethyl esters for cardiovascular prevention and treatment of increased blood triglyceride levels. Vasc Health Risk Manag 2006;2:251–262.
  7. Larsson SC, Kumlin M, Ingelman-Sundberg M, Wolk A: Dietary long-chain n–3 fatty acids for the prevention of cancer: a review of potential mechanisms. Am J Clin Nutr 2004;79:935–945.
  8. Shirai N, Suzuki H: Effects of simultaneous docosahexaenoic acid and catechin intakes on the plasma and liver lipids in low- and high-fat diet fed mice. Nutr Res 2003;23:959–969.
  9. Kobatake Y, Jinnouchi KH, Nishide E, Innami S: Differential effects of dietary eicosapentaenoic and docosahexaenoic fatty acids on lowering of triglyceride and cholesterol levels in the serum of rats on the hypercholesterolemic diet. J Nutr Sci Vitaminol 1984;30:357–372.
  10. Henry JP, Stephens-Larson P: Reduction of chronic psychosocial hypertension in mice by decaffeinated tea. Hypertension 1984;6:437–444.
  11. Hara Y: Antioxidative action of tea polyphenols; in Hara Y (ed): Green Tea. New York, Dekker, 2001, pp 26–40.
  12. Kim M, Masuda M: Cancer chemoprevention by green tea polyphenols; in Yamamoto T, Juneja LR, Chu D-C, Kim M (eds): Chemistry and Applications of Green Tea. New York, CRC Press, 1997, pp 61–73.
  13. Matsumoto N, Ishigaki F, Ishigaki A, Iwashita H, Hara Y: Reduction of blood glucose levels by tea catechin. Biosci Biotech Biochem 1993;57:525–527.
  14. Ikeda I, Imasato Y, Sasaki E, Nakayama M, Nagao H, Takeo T, Yayabe F, Sugano M: Tea catechins decrease micellar solubility and intestinal absorption of cholesterol in rats. Biochim Biophys Acta 1992;1127:141–146.
  15. Akagi M, Fukuishi N, Kan T, Sagesaka YM, Akagi R: Anti-allergic effect of tea-leaf saponin from tea leaves (Camellia sinensis var. sinensis). Biol Pharm Bull 1997;20:565–567.
  16. Hertog MG, Feskens EJ, Hollman PC, Katan MB, Kromhout D: Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet 1993;342:1007–1011.
  17. Sueoka N, Suganuma M, Sueoka E, Okabe S, Matsuyama S, Imai K, Nakachi K, Fujiki H: A new function of green tea: prevention of lifestyle-related diseases. Ann NY Acad Sci 2001;928:274–280.
  18. Kuriyama S, Shimazu T, Ohmori K, Kikuchi N, Nakaya N, Nishino Y, Tsubono Y, Tsuji I: Green tea consumption and mortality due to cardiovascular disease, cancer, and all causes in Japan: the Ohsaki Study. JAMA 2006;296:1255–1265.
  19. Shirai N, Suzuki H: Effects of Western, vegetarian, and Japanese dietary fat model diets with or without green tea extract on the plasma lipids and glucose, and liver lipids in mice. A long-term feeding experiment. Ann Nutr Metab 2004;48:95–102.
  20. Wisse BE: The inflammatory syndrome: the role of adipose tissue cytokines in metabolic disorders linked to obesity. J Am Soc Nephrol 2004;15:2792–2800.
  21. Tsai CE, Wooten JT, Otto DA: Stability of fish oil in a purified diet with added antioxidants: effects of temperature and light. Nutr Res 1989;9:673–678.

    External Resources

  22. Ide T: Interaction of fish oil and conjugated linoleic acid in affecting hepatic activity of lipogenic enzymes and gene expression in liver and adipose tissue. Diabetes 2005;54:412–423.
  23. Halminski AM, Marsh BJ, Harrison HE: Differential effects of fish oil, safflower oil and palm oil on fatty acid oxidation and glycerolipid synthesis in rat liver. J Nutr 1991;121:1554–1561.
  24. Geelen MJ, Schoots WJ, Bijleveld C, Beynen AC: Dietary medium-chain fatty acids raise and (n–3) polyunsaturated fatty acids lower hepatic triacylglycerol synthesis in rats. J Nutr 1995;125:2449–2456.
  25. Rustan AC, Nossen JO, Christiansen EN, Drevon CA: Eicosapentaenoic acid reduces hepatic synthesis and secretion of triacylglycerol by decreasing the activity of acyl-coenzyme A:1,2-diacylglycerol acyltransferase. J Lipid Res 1988;29:1417–1426.
  26. Choi YS, Goto S, Ikeda I, Sugano M: Effect of dietary n–3 polyunsaturated fatty acids on cholesterol synthesis and degradation in rats of different ages. Lipids 1989;24:45–50.
  27. Chen IS, Hotta SS, Ikeda I, Cassidy MM, Sheppard AJ, Vahouny GV: Digestion, absorption and effects on cholesterol absorption of menhaden oil, fish oil concentrate and corn oil by rats. J Nutr 1987;117:1676–1680.
  28. Smit MJ, Verkade HJ, Havinga R, Vonk RJ, Scherphof GL, In ‘t Veld G, Kuipers F: Dietary fish oil potentiates bile acid-induced cholesterol secretion into bile in rats. J Lipid Res 1994;35:301–310.
  29. Willumsen N, Hexeberg S, Skorve J, Lundquist M, Berge RK: Docosahexaenoic acid shows no triglyceride-lowering effects but increases the peroxisomal fatty acid oxidation in liver of rats. J Lipid Res 1993;34:13–22.
  30. Raederstorff DG, Schlachter MF, Elste V, Weber P: Effect of EGCG on lipid absorption and plasma lipid levels in rats. J Nutr Biochem 2003;14:326–332.
  31. Han LK, Kimura Y, Kawashima M, Takaku T, Taniyama T, Hayashi T, Zheng YN, Okuda H: Anti-obesity effects in rodents of dietary teasaponin, a lipase inhibitor. Int J Obes Relat Metab Disord 2001;25:1459–1464.
  32. Chan PT, Fong WP, Cheung YL, Huang Y, Ho WK, Chen ZY: Jasmine green tea epicatechins are hypolipidemic in hamsters (Mesocricetus auratus) fed a high-fat diet. J Nutr 1999;129:1094–1101.
  33. Diepvens K, Kovacs EM, Nijs IM, Vogels N, Westerterp-Plantenga MS: Effect of green tea on resting energy expenditure and substrate oxidation during weight loss in overweight females. Br J Nutr 2005;94:1026–1034.
  34. Koo SI, Noh SK: Green tea as inhibitor of the intestinal absorption of lipids: potential mechanism for its lipid-lowering effect. J Nutr Biochem 2007;18:179–183.
  35. Shimura T, Miura T, Usami M, Ishihara E, Tanigawa K, Ishida H, Seino Y: Docosahexanoic acid improved glucose and lipid metabolism in KK-Ay mice with genetic non-insulin-dependent diabetes mellitus. Biol Pharm Bull 1997;20:507–510.
  36. Sullivan DR, Yue DK, Capogreco C, McLennan S, Nicks J, Cooney G, Caterson I, Turtle JR, Hensley WJ: The effects of dietary n–3 fatty acid in animal models of type 1 and type 2 diabetes. Diabetes Res Clin Pract 1990;9:225–230.
  37. Guerre-Millo M: Adipose tissue and adipokines: for better or worse. Diabetes Metab 2004;30:13–19.
  38. Yamauchi T, Kamon J, Waki H, Terauchi Y, Kubota N, Hara K, Mori Y, Ide T, Murakami K, Tsuboyama-Kasaoka N, Ezaki O, Akanuma Y, Gavrilova O, Vinson C, Reitman ML, Kagechika H, Shudo K, Yoda M, Nakano Y, Tobe K, Nagai R, Kimura S, Tomita M, Froguel P, Kadowaki T: The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity. Nat Med 2001;7:941–946.
  39. Flachs P, Mohamed-Ali V, Horakova O, Rossmeisl M, Hosseinzadeh-Attar MJ, Hensler M, Ruzickova J, Kopecky J: Polyunsaturated fatty acids of marine origin induce adiponectin in mice fed a high-fat diet. Diabetologia 2006;49:394–397.
  40. Neschen S, Morino K, Rossbacher JC, Pongratz RL, Cline GW, Sono S, Gillum M, Shulman GI: Fish oil regulates adiponectin secretion by a peroxisome proliferator-activated receptor-γ-dependent mechanism in mice. Diabetes 2006;55:924–928.
  41. Anandh Babu PV, Sabitha KE, Shyamaladevi CS: Green tea extract impedes dyslipidaemia and development of cardiac dysfunction in streptozotocin-diabetic rats. Clin Exp Pharmacol Physiol 2006;33:1184–1189.
  42. Wolfram S, Raederstorff D, Preller M, Wang Y, Teixeira SR, Riegger C, Weber P: Epigallocatechin gallate supplementation alleviates diabetes in rodents. J Nutr 2006;136:2512–2518.
  43. Babu PV, Sabitha KE, Shyamaladevi CS: Green tea impedes dyslipidemia, lipid peroxidation, protein glycation and ameliorates Ca2+-ATPase and Na+/K+-ATPase activity in the heart of streptozotocin-diabetic rats. Chem Biol Interact 2006;162:157–164.
  44. Tsuneki H, Ishizuka M, Terasawa M, Wu JB, Sasaoka T, Kimura I: Effect of green tea on blood glucose levels and serum proteomic patterns in diabetic (db/db) mice and on glucose metabolism in healthy humans. BMC Pharmacol 2004;4:18.
  45. Anderson RA, Polansky MM: Tea enhances insulin activity. J Agric Food Chem 2002;50:7182–7186.
  46. Wu LY, Juan CC, Ho LT, Hsu YP, Hwang LS: Effect of green tea supplementation on insulin sensitivity in Sprague-Dawley rats. J Agric Food Chem 2004;52:643–648.
  47. Wu LY, Juan CC, Hwang LS, Hsu YP, Ho PH, Ho LT: Green tea supplementation ameliorates insulin resistance and increases glucose transporter IV content in a fructose-fed rat model. Eur J Nutr 2004;43:116–124.
  48. Gamberucci A, Konta L, Colucci A, Giunti R, Magyar JE, Mandl J, Banhegyi G, Benedetti A, Csala M: Green tea flavonols inhibit glucosidase II. Biochem Pharmacol 2006;72:640–646.
  49. Honda M, Hara Y: Inhibition of rat small intestinal sucrase and α-glucosidase activities by tea polyphenols. Biosci Biotech Biochem 1993;57:123–124.

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