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Table of Contents
Vol. 80, No. 1-4, 1998
Issue release date: 1998
Cytogenet Cell Genet 80:133–137 (1998)
(DOI:10.1159/000014969)

X-Chromosome inactivation: a repeat hypothesis

Lyon M.F.
Medical Research Council, Mammalian Genetics Unit, Harwell, Didcot, Oxon (UK)

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Abstract

Abstract.

Recent work has shown that X-chromosome inactivation is brought about by Xist mRNA, which coats the inactive X-chromosome. This paper presents a hypothesis on the function of this RNA. It is suggested that interspersed repetitive elements of the LINE type, in which the X-chromosome is particularly rich, act as booster elements to promote the spread of Xist mRNA. Contact with this RNA causes the LINE elements to be sensed as repeated elements by the cell’s system for repeat-induced gene silencing. This leads to the silencing of these elements and the intervening unique sequences by their conversion to heterochromatin.



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References

  1. Borsani G, Tonlorenzi R, Simmler MC, Dandolo L, Arnaud D, Capra V, Grompe M, Pizzuti A, Muzny D, Lawrence C, Willard HF, Avner P, Ballabio A: Characterization of a murine gene expressed from the inactive X chromosome. Nature 351:325–329 (1991).
  2. Boyle AL, Ballard SG, Ward DC: Differential distribution of long and short interspersed element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization. Proc natl Acad Sci, USA 87:7757–7761 (1990).
  3. Brockdorff N, Ashworth A, Kay GF, Cooper P, Smith S, McCabe VM, Norris DP, Penny GD, Patel D, Rastan S: Conservation of position and exclusive expression of mouse Xist from the inactive X chromosome. Nature 351:329–351 (1991).
  4. Brockdorff N, Ashworth A, Kay GF, McCabe VM, Norris DP, Cooper PJ, Swift S, Rastan S: The product of the mouse Xist gene is a 15-kb inactive X-specific transcript containing no conserved ORF and located in the nucleus. Cell 71:515–526 (1992).
  5. Brown CJ, Ballabio A, Rupert JL, Lafreniere RG, Grompe M, Tonlorenzi R, Willard HF: A gene from the region of the human X inactivation centre is expressed exclusively from the inactive X chromosome. Nature 349:33–44 (1991).
  6. Brown CJ, Hendrich BD, Rupert JL, Lafreniere RG, Xing Y, Lawrence J, Willard HF: The human XIST gene: analysis of a 17-kb inactive X-specific RNA that contains conserved repeats and is highly localised within the nucleus. Cell 71:527–542 (1992).
  7. Cattanach BM: Position effect variegation in the mouse. Genet Res 23:291–306 (1974).

    External Resources

  8. Clemson CM, McNeil JA, Willard H, Lawrence JB: XIST RNA paints the inactive X chromosome at interphase: evidence for a novel RNA involved in nuclear/chromosome structure. J Cell Biol 132:259–275 (1996).
  9. Dorer DR, Henikoff S: Expansions of transgene repeats cause heterochromatin formation and gene silencing in Drosophila. Cell 77:993–1002 (1994).

    External Resources

  10. Gartler SM, Dyer KA, Goldman MA: Mammalian X-chromosome inactivation, in Friedmann T (ed): Molecular Genetic Medicine, Vol 2 pp 121–160 (1992).
  11. Hastie ND: Highly repeated DNA families in the genome of Mus musculus, in Lyon MF, Rastan S, Brown SDM (eds): Genetic Variants and Strains of the Laboratory Mouse, 3rd edition, pp 1425–1442 (Oxford University Press 1996).
  12. Herzing LBK, Romer JT, Horn JM, Ashworth A: Xist has properties of the X-chromosome inactivation centre. Nature 386:272–275 (1997).
  13. Korenberg JR, Rykowski, MC: Human genome organization: Alu, Lines, and the molecular structure of metaphase chromosome bands. Cell 53:391–400 (1988).
  14. Lee JT, Jaenisch R: Long-range cis effects of ectopic X-inactivation centres on a mouse autosome. Nature 386:275–279 (1997).
  15. Lee JT, Strauss WM, Dausman JA, Jaenisch R: A 450-kb transgene displays properties of the mammalian X-inactivation center. Cell 86:83–94 (1996).
  16. Lyon MF: Gene action in the X-chromosome of the mouse (Mus musculus L). Nature 190:372–373 (1961).
  17. Lyon MF: Molecular genetics of X-chromosome inactivation. Adv Genome Biology 4:119–151 (1996).
  18. Marahrens Y, Panning B, Dausman J, Strauss W, Jaenisch R: Xist-deficient mice are defective in dosage compensation but not spermatogenesis. Genes Dev 11:156–166 (1997).

    External Resources

  19. Matzke MA, Matzke AJM: Homology-dependent gene silencing in transgenic plants: what does it really tell us? Trends Genet 11:1–3 (1995).

    External Resources

  20. Ohno S, Kaplan WD, Kinosita R: Formation of the sex chromatin by a single X-chromosome in liver cells of Rattus norvegicus. Exp Cell Res 18:415–418 (1959).
  21. Panning B, Dausman J, Jaenisch R: X chromosome inactivation is mediated by Xist RNA stabilization. Cell 90:907–916 (1997).
  22. Panning B, Jaenisch R: DNA hypomethylation can activate Xist expression and silence X-linked genes. Genes Dev 10:1991–2002 (1996).
  23. Penny GD, Kay GF, Sheardown SA, Rastan S, Brockdorff N: Requirement for Xist in X chromosome inactivation. Nature 379:131–137 (1996).
  24. Rastan S: Non-random X-chromosome inactivation in mouse X-autosome translocation embryos – location of the inactivation centre. J Embryol exp Morph 78:1–22 (1983).
  25. Rastan S, Robertson EJ: X-chromosome deletions in embryo-derived (EK) cell lines associated with lack of X-chromosome inactivation. J Embryol exp Morph 90:379–388 (1985).
  26. Riggs AD: Marsupials and mechanisms of X chromosome inactivation. Aust J Zool 37:419–441 (1990).
  27. Russell LB: Mammalian X-chromosome action: inactivation limited in spread and in region of origin. Science 140:976–978 (1963).
  28. Russell LB: X-autosome translocations in the mouse: their characterization and use as tools to investigate gene inactivation and gene action, in Sandberg AA (ed): Cytogenetics of the Mammalian X Chromosome, Part A. Basic Mechanisms of X Chromosome Behaviour, pp 205–250 (Alan R Liss, New York 1983).
  29. Russell LB, Bangham JW: Variegated-type position effects in the mouse. Genetics 46:509–525 (1961).
  30. Russell LB, Montgomery CS: Comparative studies on X-autosome translocations in the mouse II. Inactivation of autosomal loci, segregation, and mapping of autosomal breakpoints in five T(X;1)’s. Genetics 64:281–312 (1970).

    External Resources

  31. Searle AG, Beechey CV, Evans EP, Kirk M: Two new X-autosome translocations in the mouse. Cytogenet Cell Genet 35:279–282 (1983).
  32. Selker EV: Epigenetic phenomena in filamentous fungi: useful paradigms or repeat-induced confusion? Trends Genet 13:296–301 (1997).

    External Resources

  33. Sheardown SA, Duthie SM, Johnston CM, Newall AET, Formstone EJ, Arkell RM, Nesterova TB, Alghisi G-C, Rastan S, Brockdorff N: Stabilization of Xist RNA mediates initiation of X chromosome inactivation. Cell 91:99–107 (1997).

    External Resources

  34. Wolffe AP: Transcription control: repressed repeats express themselves. Current Biology 7:R796–R798 (1997).

    External Resources

  35. Yoder JA, Walsh CP, Bestor TH: Cytosine methylation and the ecology of intragenomic parasites. Trends Genet 13:335–340 (1997).

    External Resources



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