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Vol. 85, No. 3-4, 1999
Issue release date: 1999
Section title: Paper
Cytogenet Cell Genet 85:205–211 (1999)
(DOI:10.1159/000015294)

Construction of a swine BAC library: application to the characterization and mapping of porcine type C endoviral elements

Rogel-Gaillard C. · Bourgeaux N. · Billault A. · Vaiman M. · Chardon P.
aLaboratoire de Radiopathologie et d’Etude du Génome, Département de Génétique Animale, Jouy-en-Josas; bFondation Jean Dausset, Centre d’Etude du Polymorphisme Humain, Paris; and cLaboratoire de Radiopathologie et d’Etude du Génome, DSV, DRR, LRA, Jouy-en-Josas (France)

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Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 8/6/1999

Number of Print Pages: 7
Number of Figures: 6
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR

Abstract

Abstract.

A porcine bacterial artificial chromosome (BAC) library was constructed using the pBeloBAC11 vector. It comprised 107,520 clones with an average insert size of 135 kb, representing an almost fivefold coverage of the swine haploid genome. Screening of the library allowed recovery of one to eight clones for 142 unique markers located all over the genome, while it failed for only one marker. About 4% chimeric clones were found. The library was also screened for the protease gene of type C porcine endoviral sequences (PERVs), and 62 clones were recovered, all but two of which contained one protease gene. We found 20 protease sequences (PERV-1 to PERV-20) which, despite differing by point mutations, were all coding sequences. The most frequent sequence, PERV-2, was 100% similar to a protease sequence expressed in the porcine PK-15 cell line. Most of the clones harbored envelope genes. Thirty-three BAC clones were mapped by fluorescence in situ hybridization to 22 distinct locations on 14 chromosomes, including the X and Y chromosomes. These overall results indicate that there is generally one PERV copy per integration site. Although PERV sequences were not tandemly arranged, clusters of integration sites were observed at positions 3p1.5 and 7p1.1. Southern blot experiments revealed 20–30 PERV copies in the Large White pig genome studied here, and variations in PERV content among pigs of different breeds were observed. In conclusion, this BAC collection represents a significant contribution to the swine large genomic DNA cloned insert resources and provides the first detailed map of PERV sequences in the swine genome. This work is the first step toward identification of potential active sites of PERV elements.


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 8/6/1999

Number of Print Pages: 7
Number of Figures: 6
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: http://www.karger.com/CGR


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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