Differentiation of Madin-Darby Canine Kidney Cells Depends on Cell Culture ConditionsKersting U. · Schwab A. · Treidtel M. · Pfaller W. · Gstraunthaler G. · Steigner W. · Oberleithner H.
Department of Physiology, University of Würzburg, FRG
We used Madin-Darby canine kidney (MDCK) cells as a model to study changes in renal epithelial cell structure and function induced by different environments. We identiñed two cell types, MDCK-1 and MDCK-2, that differ in structure, function and lectin binding. MDCK cells differentiate into MDCK-1 cells in acid and hypertonic culture media, whereas MDCK-2 cells develop in alkaline and hypotonic culture media in subconfluent and young confluent monolayers. Fully differentiated MDCK-1 and MDCK-2 cells cannot reverse their differentiation state in aged confluent monolayers. Relative sodium conductance is increased 48-fold in MDCK-1 cells. MDCK-1 cells acidify their incubation medium, whereas MDCK-2 cells acidify dome fluid. Hypotonic stress further decreases HCO–3 concentration in dome fluid. Dome formation increases in higher cell passages; concomitantly the ratio of MDCK-2:MDCK-1 cells increases. Cloned MDCK cell populations become increasingly heterogenous with increasing passage number. We conclude that differentiation in MDCK cells is determined by cell culture conditions.
|Direct payment||This item at the regular price: USD 38.00|
|Payment from account||With a Karger Pay-per-View account (down payment USD 150)
you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50