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Vol. 88, No. 1-2, 2000
Issue release date: 2000
Cytogenet Cell Genet 88:159–162 (2000)

Characterization of a new TSPY gene family member in Yq (TSPYq1)

Ratti A. · Stuppia L. · Gatta V. · Fogh I. · Calabrese G. · Pizzuti A. · Palka G.
aIstituto di Clinica Neurologica, Università di Milano, Ospedale Policlinico IRCCS; bDipartimento di Scienze Biomediche, Sezione di Genetica Medica, Università “G. D’Annunzio,” Chieti; cIstituto di Citomorfologia Umana Normale e Patologica CNR, Chieti; dServizio di Genetica Umana, Ospedale Civile di Pescara; and eC.S.S.-Mendel Institute IRCCS, San Giovanni Rotondo (Italy).

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We investigated subinterval 6E on the human Y chromosome, a region frequently deleted in infertile males. YAC yOX17, mapped within subinterval 6E by STS-PCR, was analyzed for the presence of new genes. TSPYq1, a member of the TSPY multi-copy gene family, was isolated and character- ized from a yOX17 cosmid subclone. PCR and FISH analysis performed on normal subjects and on patients with microdeletions of Yq suggested the presence of multiple copies of TSPY in Yq.   

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