Journal Mobile Options
Table of Contents
Vol. 90, No. 1-2, 2000
Issue release date: 2000
Cytogenet Cell Genet 90:47–52 (2000)
(DOI:10.1159/000015658)

Human secretin (SCT): gene structure, chromosome location, and distribution of mRNA

Whitmore T.E. · Holloway J.L. · Lofton-Day C.E. · Maurer M.F. · Chen L. · Quinton T.J. · Vincent J.B. · Scherer S.W. · Lok S.
aZymoGenetics Inc., Seattle, WA (USA), and bDepartment of Genetics, The Hospital for Sick Children, Toronto, Ont. (Canada)

Individual Users: Register with Karger Login Information

Please create your User ID & Password





Contact Information











I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Abstract

Abstract.

Secretin is an endocrine hormone that stimulates the secretion of bicarbonate-rich pancreatic fluids. Recently, it has been discussed that secretin deficiency may be implicated in autistic syndrome, suggesting that the hormone could have a neuroendocrine function in addition to its role in digestion. In the present study, the human secretin gene (SCT) was isolated from a bacterial artificial chromosome genomic library. SCT contains four exons, with the protein coding regions spanning 713 bp of genomic DNA. Human SCT is similar structurally to the secretin genes of other species. Amino acid conservation, however, is most pronounced within the exon encoding the biologically active mature peptide. Northern blot analysis shows that human SCT transcripts are located in the spleen, intestinal tract, and brain. Radiation hybrid mapping places the SCT locus on chromosome 11p15.5.   

Copyright © 2000 S. Karger AG, Basel



Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

References

  1. Bayliss WM, Starling EH: The mechanism of pancreatic secretion. J Physiol 28:325–353 (1902).
  2. Berg JM: Sp1 and the subfamily of zinc finger proteins with guanine-rich binding sites. Proc natl Acad Sci, USA 89:11109–11110 (1992).
  3. Bloom SR: Hormones of gastrointestinal-tract. Br med Bull 30:62–67 (1974).

    External Resources

  4. Breathnach R, Chambon P: Organization and expression of eukaryotic genes coding for proteins. A Rev Biochem 50:349–383 (1981).
  5. Buscail L, Cauvin A, Gourlet P, Gossen D, DeNeef P, Rathe J, Robberecht P, Vandermeers-Piret M-C, Vandermeers A, Christophe J: Purification and amino acid sequence of vasoactive intestinal peptide, peptide histidine isoleucinamide (1-27) and secretin from the small intestine of guinea pig. Biochim biophys Acta 1038:355–359 (1990).
  6. Carlquist M, Jornvall H, Forssmann W-G, Thulin L, Johansson C, Mutt V: Human secretin is not identical to the porcine bovine hormone. IRCS med Sci 13:217–218 (1985).
  7. Carlquist M, Jornvall H, Mutt V: Isolation and amino acid sequence of bovine secretin. FEBS Lett 127:71–74 (1981).
  8. Chang T, Berger-Ornstein L, Chey WY: Presence of biologically and immunologically active secretin-like substance in the mammalian brain. Peptides 6:193–198 (1985).
  9. Fara JW, Madden KS: Effect of secretin and cholecystokinin on small intestinal blood flow distribution. Am J Physiol 229:1365–1370 (1975).

    External Resources

  10. Gafvelin G, Jornvall H, Mutt V: Processing of presecretin: isolation of a secretin precursor from porcine intestine. Proc natl Acad Sci, USA 87:6781–6785 (1990).

    External Resources

  11. Gossen D, Buscail L, Cauvin A, Gourlet P, DeNeef P, Rathe J, Robberecht P, Vandermeers A, Vandermeers-Piret M-C, Christophe J: Amino acid sequence of VIP, PHI and secretin from rabbit small intestine. Peptides 11:123–128 (1990).
  12. Gossen D, Vandermeers A, Vandermeers-Piret M-C, Rathe J, Robberecht P, Christophe J: Isolation and primary structure of rat secretin. Biochem biophys Res Commun 160:862–867 (1989).
  13. Heinrich G, Gros P, Habener JF: Glucagon gene sequence. Four of six exons encode separate functional domain of rat pre-proglucagon. J biol Chem 259:14082–14087 (1984).

    External Resources

  14. Horvath K, Papadimitriou JC, Rabsztyn A, Drachenberg C: Gastrointestinal abnormalities in children with autistic disorder. J Pediat 135:559–563 (1999).
  15. Horvath K, Stefanatos G, Sokolski KN, Wachtel R, Nabors L, Tildon JT: Improved social and language skills after secretin administration in patients with autistic spectrum disorders. J Assoc Academic Minority Physicians 9:9–15 (1998).
  16. Hu YF, Luscher LB, Admon A, Mermod N, Tjian R: Transcription factor AP-4 contains multiple dimerization domains that regulate dimer specificity. Genes Dev 4:1741–1752 (1990).

    External Resources

  17. Ichihara K, Eng J, Yalow RS: Ontogeny of immunoreactive CCK, VIP and secretin in rat brain and gut. Biochem biophys Res Commun 112:891–898 (1983).
  18. Imagawa M, Chiu R, Karin M: Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP. Cell 51:251–260 (1987).
  19. Inagaki N, Seino Y, Takeda J, Yano H, Yamada Y, Bell GI, Eddy RL, Fukushima Y, Byers MG, Shows TB, Imura H: Gastric inhibitory polypeptide: structure and chromosomal localization of the human gene. Mol Endocr 3:1014–1021 (1989).

    External Resources

  20. Itoh N, Furuya T, Ozaki K, Ohta M, Kawasaki T: The secretin precursor gene. J biol Chem 266:12595–12598 (1991).
  21. Jorpes J, Mutt V: On the biological activity and amino acid composition of secretin. Acta chem scand 15:1790–1791 (1961).
  22. Karlsson O, Edlund T, Moss JB, Rutter WJ, Walker MD: A mutational analysis of the insulin gene transcription control region: expression in beta cells is dependent on two related sequences within the enhancer. Proc natl Acad Sci, USA 84:8819–8823 (1987).

    External Resources

  23. Koplin AS, Wheeler MB, Leiter AB: Secretin, structure of the precursor and tissue distribution. Proc natl Acad Sci, USA 87:2299–2303 (1990).

    External Resources

  24. Koplin AS, Wheeler MB, Nishitani J, McBride DW, Chang T-M, Chey WY, Leiter AB: The secretin gene: evolutionary history, alternative splicing, and developmental regulation. Proc natl Acad Sci, USA 88:5335–5339 (1991).

    External Resources

  25. Kreil G: Protein Compartmentalization, pp 61–70 (Springer-Verlag, New York 1986).
  26. Lan MS, Kajiyama W, Donadel G, Ku J, Notkins AL: cDNA sequence and genomic organization of mouse secretin. Biochem biophys Res Commun 200:1066–1071 (1994).
  27. Leiter AB, Chey WY, Koplin AS: Secretin, in Walsh JH, Dockray GJ (eds): Gut Peptides, pp 147–174 (Raven Press, New York 1993).
  28. Linder S, Narkhem T, Norberg A, Persson H, Schalling M, Hokfelt T, Magnusson G: Structure and expression of the gene encoding the vasoactive intestinal peptide precursor. Proc natl Acad Sci, USA 84:605–609 (1987).
  29. Mayo KE, Cerelli GM, Rosenfeld MG, Evans RM: Characterization of cDNA and genomic clones encoding the precursor to rat hypothalamic growth hormone-releasing factor. Nature 314:464–467 (1985).
  30. Mitchell PJ, Wang C, Tjian R: Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen. Cell 50:847–861 (1987).
  31. Mutt V, Carlquist M, Tatemoto K: Secretin-like bioactivity in extracts of porcine brain. Life Sci 25:1703–1707 (1979).
  32. Mutt V, Jorpes JE, Magnusson S: Structure of porcine secretin: the amino acid sequence. Eur J Biochem 15:513–519 (1970).

    External Resources

  33. Nishitani J, Rindi G, Lopez MJ, Upchurch BH, Leiter AB: Transcriptional regulation of secretin gene expression. J Clin Gastroenterol 21(Suppl 1):S50–55 (1995).
  34. O’Donohue TL, Charlton CG, Miller RL, Boden G, Jacobowitz DM: Identification, characterization and distribution of secretin immunoreactivity in rat and pig brain. Proc natl Acad Sci, USA 78:5221–5224 (1981).

    External Resources

  35. Ohta M, Funakoshi S, Kawasaki T, Itoh N: Tissue-specific expression of the rat secretin precursor gene. Biochem biophys Res Commun 183:390–395 (1992).
  36. Paquette TL, Schulman DF, Alpers DH, Jaffe BM: Postnatal development of intestinal secretin in rat and guinea pigs. Am J Physiol 243:G511–517 (1982).

    External Resources

  37. Pollack PF, Wood JG, Solomon T: Effect of secretin on growth of stomach, small intestine, and pancreas of developing rats. Dig Dis Sci 35:749–758 (1990).
  38. Shinomura Y, Eng J, Yalow RS: Dog secretin: sequence and biologic activity. Life Sci 41:1243–1248 (1987).
  39. Straus E, Yalow RS: Immunoreactive secretin in gastrointestinal mucosa of several mammalian species. Gastroenterology 75:401–404 (1978).

    External Resources

  40. Tsukada T, Horovitch S, Montminy M, Mandel G, Goodman RH: Structure of the human vasoactive intestinal polypeptide gene. DNA 4:293–300 (1985).

    External Resources

  41. Wheeler MB, Nishitani J, Buchan AM, Koplin AS, Vhey WY, Chang T-M, Leiter AB: Identification of a transcriptional enhancer important for enteroendocrine and pancreatic islet cell-specific expression of the secretin gene. Mol Cell Biol 12:3531–3539 (1992).

    External Resources

  42. Yamagata T, Aradhya S, Mori M, Momoi MY, Nelson DL: Secretin gene structure, chromosome localization and mutation analysis on autism. Am J hum Genet 65(Suppl):A473 (1999).
  43. Yanaihara N, Sakagami M, Sato H, Yammamoto K, Hashimoto T: Immunological aspects of secretin, substance P, and VIP. Gastroenterology 72:803–810 (1977).

    External Resources



Pay-per-View Options
Direct payment This item at the regular price: USD 38.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50