The aim of this study was to examine the colonization of Streptococcus mutans and Streptococcus sanguinis in the oral cavity and the association with severe early childhood caries (S-ECC). Saliva and plaque samples were collected from 14 S-ECC children and 8 caries-free (CF) children. All S-ECC children were S. mutans positive; 100% of CF children and 93% of S-ECC children were S. sanguinis positive. The children’s caries severity was positively correlated with levels of S. mutans (p < 0.001), total oral streptococci (p < 0.01), total cultivable oral bacteria (p < 0.05), and children’s age (p < 0.05). Logistic regression analysis showed that the interaction of S. sanguinis with S. mutans was a significant factor associated with the caries status in children, suggesting that the relative levels of these two microorganisms in the oral cavity play an important role in caries development.
© 2008 S. Karger AG, Basel
- Early childhood caries
- Streptococcus mutans
- Streptococcus sanguinis
- Alaki SM, Loesche WJ, da Fonesca MA, Feigal RJ, Welch K: Preventing the transfer of Streptococcus mutans from primary molars to permanent first molars using chlorhexidine. Pediatr Dent 2002;24:103–108.
- Babaahmady KG, Challacombe SJ, Marsh PD, Newman HN: Ecological study of Streptococcus mutans,Streptococcus sobrinus and Lactobacillus spp at sub-sites from approximal dental plaque from children. Caries Res 1998;32:51–58.
- Becker MR, Paster BJ, Leys EJ, Moeschberger ML, Kenyon SG, Galvin JL, Boches SK, Dewhirst FE, Griffen AL: Molecular analysis of bacterial species associated with childhood caries. J Clin Microbiol 2002;40:1001–1009.
- Beighton D, Brailsford S, Samaranayake LP, Brown JP, Ping FX, Grant-Mills D, Harris R, Lo EC, Naidoo S, Ramos-Gomez F, Soo TC, Burnside G, Pine CM: A multi-country comparison of caries-associated microflora in demographically diverse children. Community Dent Health 2004;21:96–101.
- Berkowitz R: Etiology of nursing caries: a microbiologic perspective. J Public Health Dent 1996;56:51–54.
- Burt BA, Loesche WJ, Eklund SA: Stability of selected plaque species and their relationship to caries in a child population over 2 years. Caries Res 1985;19:193–200.
- Caufield PW, Cutter GR, Dasanayake AP: Initial acquisition of mutans streptococci by infants: evidence for a discrete window of infectivity. J Dent Res 1993;72:37–45.
- Caufield PW, Dasanayake AP, Li Y, Pan Y, Hsu J, Hardin JM: Natural history of Streptococcus sanguinis in the oral cavity of infants: evidence for a discrete window of infectivity. Infect Immun 2000;68:4018–4023.
- Corby PM, Lyons-Weiler J, Bretz WA, Hart TC, Aas JA, Boumenna T, Goss J, Corby AL, Junior HM, Weyant RJ, Paster BJ: Microbial risk indicators of early childhood caries. J Clin Microbiol 2005;43:5753–5759.
- Dasanayake AP, Caufield PW, Cutter GR, Roseman JM, Kohler B: Differences in the detection and enumeration of mutans streptococci due to differences in methods. Arch Oral Biol 1995;40:345–351.
- De Stoppelaar JD, Van Houte J, Backer Dirks O: The relationship between extracellular polysaccharide-producing streptococci and smooth surface caries in 13-year-old children. Caries Res 1969;3:190–199.
- Hamada S, Slade HD: Biology, immunology, and cariogenicity of streptococcus mutans. Microbiol Rev 1980;44:331–384.
- Kohler B, Andreen I, Jonsson B: The earlier the colonization by mutans streptococci, the higher the caries prevalence at 4 years of age. Oral Microbiol Immunol 1988;3:14–17.
- Li Y, Caufield P, Emanuelsson I, Thornqvist E: Differentiation of Streptococcus mutans and Streptococcus sobrinus via genotypic and phenotypic profiles from three different populations. Oral Microbiol Immunol 2001;16:16–23.
- Li Y, Caufield PW: The fidelity of initial acquisition of mutans streptococci by infants from their mothers. J Dent Res 1995;74:681–685.
- Li Y, Caufield PW, Dasanayake AP, Wiener HW, Vermund SH: Mode of delivery and other maternal factors influence the acquisition of Streptococcus mutans in infants. J Dent Res 2005;84:806–811.
- Li Y, Ge Y, Saxena D, Caufield PW: Genetic profiling of the oral microbiota associated with severe early-childhood caries. J Clin Microbiol 2007;45:81–87.
- Li Y, Navia JM, Caufield PW: Colonization by mutans streptococci in the mouths of 3- and 4-year-old Chinese children with or without enamel hypoplasia. Arch Oral Biol 1994;39:1057–1062.
- Li Y, Wang W, Caufield PW: The fidelity of mutans streptococci transmission and caries status correlate with breast-feeding experience among chinese families. Caries Res 2000;34:123–132.
- Lindquist B, Emilson CG: Colonization of Streptococcus mutans and Streptococcus sobrinus genotypes and caries development in children to mothers harboring both species. Caries Res 2004;38:95–103.
- Loesche WJ: Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986;50:353–380.
- Loesche WJ, Eklund S, Earnest R, Burt B: Longitudinal investigation of bacteriology of human fissure decay: epidemiological studies in molars shortly after eruption. Infect Immun 1984;46:765–772.
- Loesche WJ, Walenga A, Loos P: Recovery of Streptococcus mutans and Streptococcus sanguis from a dental explorer after clinical examination of single human teeth. Arch Oral Biol 1973;18:571–575.
- Marchant S, Brailsford SR, Twomey AC, Roberts GJ, Beighton D: The predominant microflora of nursing caries lesions. Caries Res 2001;35:397–406.
- Matee MI, Mikx FH, Maselle SY, Van Palenstein Helderman WH: Mutans streptococci and lactobacilli in breast-fed children with rampant caries. Caries Res 1992;26:183–187.
- Okada M, Soda Y, Hayashi F, Doi T, Suzuki J, Miura K, Kozai K: PCR detection of Streptococcus mutans and S. sobrinus in dental plaque samples from Japanese pre-school children. J Med Microbiol 2002;51:443–447.
- Okada M, Soda Y, Hayashi F, Doi T, Suzuki J, Miura K, Kozai K: Longitudinal study of dental caries incidence associated with Streptococcus mutans and Streptococcus sobrinus in pre-school children. J Med Microbiol 2005;54:661–665.
- Pan YP, Li Y, Caufield PW: Phenotypic and genotypic diversity of Streptococcus sanguis in infants. Oral Microbiol Immunol 2001;16:235–242.
- Seki M, Yamashita Y, Shibata Y, Torigoe H, Tsuda H, Maeno M: Effect of mixed mutans streptococci colonization on caries development. Oral Microbiol Immunol 2006;21:47–52.
- Thurnheer T, Gmur R, Giertsen E, Guggenheim B: Automated fluorescent in situ hybridization for the specific detection and quantification of oral streptococci in dental plaque. J Microbiol Methods 2001;44:39–47.
Dr. Yihong Li
New York University College of Dentistry
345 E. 24th Street
New York, NY 10010-4086 (USA)
Tel. +1 212 998 9607, Fax +1 212 995 4087, E-Mail email@example.com
Received: November 20, 2007
Accepted after revision: August 6, 2008
Published online: October 3, 2008
Number of Print Pages : 5
Number of Figures : 0, Number of Tables : 3, Number of References : 30
Vol. 42, No. 6, Year 2008 (Cover Date: November 2008)
Journal Editor: Shellis R.P. (Bristol)
ISSN: 0008–6568 (Print), eISSN: 1421–976X (Online)
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