J Innate Immun 2009;1:358–365

Blockade of LOX-1 Prevents Endotoxin-Induced Acute Lung Inflammation and Injury in Mice

Zhang P.a · Liu M.-C.b · Cheng L.a · Liang M.a · Ji H.a · Fu J.a
aCenter for Biomedical Research, University of Texas Health Science Center at Tyler, Tyler, Tex., and bDepartment of Pharmacology, University of Toledo, Toledo, Ohio, USA
email Corresponding Author

 goto top of outline Key Words

  • Endotoxin
  • Inflammation
  • LOX-1
  • Lung
  • Neutrophil

 goto top of outline Abstract

Lectin-like oxidized low-density lipoprotein (LDL) receptor-1 (LOX-1), a cell surface receptor expressed in endothelial cells, is known to mediate oxidized LDL-induced vascular inflammation and atherogenesis. Although the role of LOX-1 in vascular inflammation has been well established, its involvement in acute lung inflammation and injury remains unclear. In the present study, we examined the effects of a LOX-1-blocking antibody on lung inflammation in a mouse endotoxin lipopolysaccharide (LPS)-induced acute lung injury model. We demonstrated that intraperitoneal challenge with LPS induced a rapid and robust increase in LOX-1 expression in mouse lung. Pre-treatment of mice with anti-LOX-1-blocking antibody significantly inhibited LPS-induced lung inflammation as indicated by decreased neutrophil accumulation in the lung. Furthermore, anti-LOX-1 was capable of inhibiting LPS-induced inflammatory responses, including NF-κB activation, ICAM-1 expression and apoptotic signaling, in mouse lung. Collectively, these results indicate that LOX-1 may serve as a valuable therapeutic target in the prevention of acute lung inflammation and injury in sepsis.

Copyright © 2008 S. Karger AG, Basel

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 goto top of outline Author Contacts

Dr. Jian Fu
Department of Biochemistry, Center for Biomedical Research
University of Texas Health Science Center at Tyler
11937 US Highway 271, Tyler, TX 75708 (USA)
Tel. +1 903 877 7942, Fax +1 903 877 5914, E-Mail jian.fu@uthct.edu

 goto top of outline Article Information

Received: June 16, 2008
Accepted after revision: August 5, 2008
Published online: October 1, 2008
Number of Print Pages : 8
Number of Figures : 6, Number of Tables : 0, Number of References : 40

 goto top of outline Publication Details

Journal of Innate Immunity

Vol. 1, No. 4, Year 2009 (Cover Date: April 2009)

Journal Editor: Herwald H. (Lund), Egesten A. (Lund)
ISSN: 1662-811X (Print), eISSN: 1662-8128 (Online)

For additional information: http://www.karger.com/JIN

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