- Quantitative analysis
- Thin-layer chromatography
- Artocarpus lakoocha
Objective: To develop a thin-layer chromatography (TLC) densitometric method for the determination of oxyresveratrol content in Artocarpuslakoocha heartwood and in the traditional drug ‘Puag-Haad’. Materials and Methods: Sample solution of A. lakoocha heartwood was prepared by Soxhlet extraction of the plant material in ethanol, whereas the Puag-Haad solution was obtained by dissolving the drug in methanol. Analysis of each sample solution was performed on a Silica gel 60 F254 TLC plate (20 × 10 cm) with methylene chloride/methanol (85:15) as the mobile phase. After development, the TLC plate was examined with a TLC scanner in the absorbance mode at 254 nm. The newly developed analytical method was validated using an authentic sample of oxyresveratrol previously isolated from A. lakoocha heartwood, and was used to analyze the oxyresveratrol content in samples of A. lakoocha heartwood and the traditional drug Puag-Haad. Results: A sensitive and reliable TLC densitometric method was successfully developed. The method was validated in terms of accuracy (99.11–102.60%) and precision (1.66–4.23% coefficient of variation). The limits of detection and quantitation were 15.6 and 52 ng/spot, respectively. The amounts of oxyresveratrol in 3 samples of A. lakoocha heartwood collected from its natural habitat were 49.0–182.3 mg/g, whereas those in 11 commercial samples were in the range of 23.4–69.6 mg/g. The oxyresveratrol contents in 2 samples of traditional drug Puag-Haad were 780.1 and 837.5 mg/g. Conclusion: The TLC densitometric method developed in this study is a simple, convenient, sensitive and reliable procedure. It was an effective analytical tool for the evaluation of oxyresveratrol content in both A. lakoocha heartwood and the traditional drug Puag-Haad.
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Department of Pharmacognosy, Faculty of Pharmaceutical Sciences
Bangkok 10330 (Thailand)
Tel. +66 2 218 8360, Fax +66 2 218 8357, E-Mail Kittisak.L@chula.ac.th
Received: May 28, 2008
Revised: September 17, 2008
Published online: April 06, 2009
Number of Print Pages : 5
Number of Figures : 3, Number of Tables : 3, Number of References : 18
Medical Principles and Practice (International Journal of the Kuwait University Health Sciences Centre)
Vol. 18, No. 3, Year 2009 (Cover Date: April 2009)
Journal Editor: Owunwanne A. (Kuwait)
ISSN: 1011-7571 (Print), eISSN: 1423-0151 (Online)
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