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Vol. 149, Suppl. 1, 2009
Issue release date: June 2009
Int Arch Allergy Immunol 2009;149(suppl 1):21–24

Development of Transgenic Rice Expressing Mite Antigen for a New Concept of Immunotherapy

Suzuki K. · Kaminuma O. · Yang L. · Motoi Y. · Takai T. · Ichikawa S. · Okumura K. · Ogawa H. · Mori A. · Takaiwa F. · Hiroi T.
aDepartment of Allergy and Immunology, The Tokyo Metropolitan Institute of Medical Science, Tokyo, bTransgenic Crop Research and Development Center, National Institute of Agrobiological Sciences, Tsukuba, cAtopy (Allergy) Research Center, Juntendo University School of Medicine, Tokyo, and dClinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital, Sagamihara, Japan

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Background: To elucidate the usefulness of antigen-specific immunotherapy based on oral vaccination with an edible part of the plant, we examined the effect of transgenic (Tg) rice seeds expressing an immunodominant fragment of the group 1 antigen of Dermatophagoides pteronyssinus (Der p 1) on a murine model of asthma. Methods: Mice were orally vaccinated with the Tg or non-Tg rice seeds for 2 weeks, then they were immunized with recombinant Der p 1 (rDer p 1) and alum intraperitoneally. Antigen-induced immune responses, such as proliferation and cytokine production of CD4+ T cells, antigen-specific serum IgE and IgG, and infiltration of inflammatory cells into the airways were investigated in those mice. Results: The proliferation and Th2 cytokine production of CD4+ T cells in vitro, antigen-specific IgE and IgG synthesis as well as accumulation of eosinophils and lymphocytes into the airways in vivo were significantly inhibited by administration of the Tg rice. Conclusions: These results suggest that the edible vaccines using Tg rice seeds are useful for the treatment of allergic disorders including bronchial asthma.

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  1. Takagi H, Hiroi T, Yang L, Tada Y, Yuki Y, Takamura K, Ishimitsu R, Kawauchi H, Kiyono H, Takaiwa F: A rice-based edible vaccine expressing multiple T cell epitopes induces oral tolerance for inhibition of Th2-mediated IgE responses. Proc Natl Acad Sci USA 2005;102:17525–17530.
  2. Takaiwa F: A rice-based edible vaccine expressing multiple T-cell epitopes to induce oral tolerance and inhibit allergy. Immunol Allergy Clin North Am 2007;27:129–139.
  3. Platts-Mills TA, Chapman MD: Dust mites: immunology, allergic disease, and environmental control. J Allergy Clin Immunol 1987;80:755–775.
  4. Thomas WR, Smith W: House-dust-mite allergens. Allergy 1998;53:821–832.
  5. Kawamoto S, Aki T, Yamashita M, Tategaki A, Fujimura T, Tsuboi S, Katsutani T, Suzuki O, Shigeta S, Murooka Y, Ono K: Toward elucidating the full spectrum of mite allergens – state of the art. J Biosci Bioeng 2002;94:285–298.
  6. Ghaemmaghami AM, Robins A, Gough L, Sewell HF, Shakib F: Human T cell subset commitment determined by the intrinsic property of antigen: the proteolytic activity of the major mite allergen Der p 1 conditions t cells to produce more IL-4 and less IFN-γ. Eur J Immunol 2001;31:1211–1216.
  7. Yssel H, Johnson KE, Schneider PV, Wideman J, Terr A, Kastelein R, De Vries JE: T cell activation-inducing epitopes of the house dust mite allergen Der p 1. Proliferation and lymphokine production patterns by Der p 1-specific CD4+ T cell clones. J Immunol 1992;148:738–745.
  8. O’Brien RM, Thomas WR, Tait BD: An immunogenetic analysis of T-cell reactive regions on the major allergen from the house dust mite, Der p 1, with recombinant truncated fragments. J Allergy Clin Immunol 1994;93:628–634.
  9. Yang L, Kajiura H, Suzuki K, Hirose S, Fujiyama K, Takaiwa F: Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy. Biochem Biophys Res Commun 2008;365:334–339.
  10. Aydogan M, Mete N, Yazi D, Akkoc T, Ozdemir C, Blaser K, Akdis M, Akdis C, Bahceciler N, Barlan I: Comparison of Der p 1-specific antibody levels in children with allergic airway disease and healthy controls. Pediatr Allergy Immunol 2007;18:320–325.
  11. Kips JC: Cytokines in asthma. Eur Respir J Suppl 2001;34:24S–33S.
  12. Nakajima H, Takatsu K: Role of cytokines in allergic airway inflammation. Int Arch Allergy Immunol 2007;142:265–273.
  13. Kikuchi Y, Takai T, Kuhara T, Ota M, Kato T, Hatanaka H, Ichikawa S, Tokura T, Akiba H, Mitsuishi K, Ikeda S, Okumura K, Ogawa H: Crucial commitment of proteolytic activity of a purified recombinant major house dust mite allergen Der p 1 to sensitization toward IgE and IgG responses. J Immunol 2006;177:1609–1617.

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