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Vol. 16, No. 4, 2009
Issue release date: May 2009
Neuroimmunomodulation 2009;16:237–244
(DOI:10.1159/000212384)

Exercise Intensity-Dependent Changes in the Inflammatory Response in Sedentary Women: Role of Neuroendocrine Parameters in the Neutrophil Phagocytic Process and the Pro-/Anti-Inflammatory Cytokine Balance

Giraldo E. · Garcia J.J. · Hinchado M.D. · Ortega E.
Department of Physiology, Faculty of Sciences, University of Extremadura, Badajoz, Spain

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Abstract

Background: It is still not really known what is the optimal level of exercise that improves, but does not impair or overstimulate the innate immune function. This is especially the case in women, who have higher basal levels of ‘inflammatory markers’ than men. The aim of this work was to evaluate differences in the magnitude of the stimulation of the innate/inflammatory response following a single bout of moderate or intense exercise in sedentary women, all of them in the follicular phase of their menstrual cycle. Changes in stress and sexual hormones were also evaluated. Methods: Changes induced by exercise (45 min at 55% VO2 max vs. 1 h at 70% VO2 max on a cycle ergometer) in the phagocytic process (chemotaxis, phagocytosis, and microbicide capacity against Candida albicans) and in serum concentrations of IL-1β, IL-2, IFN-γ, IL-12, IL-6, and IL-4 (ELISA) were evaluated. Parallel determinations were also made of serum or plasma concentrations of catecholamines (HPLC) and cortisol, oestradiol, and progesterone (electrochemiluminescence immunoassay). Results: Both exercise intensities increased chemotaxis, phagocytosis, and microbicide capacity of the neutrophils. However, the increase in chemotaxis was greater after moderate exercise. All the cytokines assayed were affected by exercise intensity. IFN-γ increased significantly only immediately after the intense exercise; IL-1β increased following both exercise intensities, although at 24 h it only remained elevated after the intense exercise; IL-12 only increased 24 h after the intense exercise, and IL-2 only showed a significant decrease following the moderate exercise. IL-6 increased immediately after both exercise intensities, but more so after moderate exercise. While IL-4 (an anti-inflammatory cytokine) increased following the moderate exercise, it decreased after the intense exercise. Both moderate and intense exercise increased norepinephrine and decreased cortisol, both of which returned to basal levels after 24 h. Only the intense exercise affected the epinephrine, oestradiol, and progesterone concentrations, with increases in epinephrine and oestradiol immediately after exercise, and a decrease in progesterone after 24 h. Conclusions: Both moderate and intense exercise stimulate the phagocytic process of neutrophils in sedentary women, but the profile of pro-/anti-inflammatory cytokine release seems to be better following the moderate exercise. The possible participation of stress (catecholamines and cortisol) and sex (oestradiol and progesterone) hormones in these intensity-dependent immune changes is discussed.



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References

  1. Ortega E: Neuroendocrine mediators in the modulation of phagocytosis by exercise: physiological implications. Exerc Immunol Rev 2003;9:70–94.
  2. Meksawan K, Venkatraman JT, Awad AB, Pendergast DR: Effect of dietary fat intake and exercise on inflammatory mediators of the immune system in sedentary men and women. J Am Coll Nutr 2004;23:331–340.
  3. Elenkov IJ, Chrousos GP: Stress hormones, proinflammatory and antiinflammatory cytokines, and autoimmunity. Ann NY Acad Sci 2002;966:290–303.
  4. Wilder R, Elenkov I: Ovarian and sympathoadrenal hormones, pregnancy and autoimmunity diseases; in Ader R, Felten DL, Cohen N (eds): Psyconeuroimmunology, ed 3. London, Academic Press, 2001, p 421.
  5. Ortega E, Giraldo E, Hinchado MD, Martin L, Garcia JJ, de la Fuente M: Neuroimmunomodulation during exercise: role of catecholamines as ‘stress mediator’ and/or ‘danger signal’ for the innate immune response. Neuroimmunomodulation 2007;14:206–212.
  6. Giraldo E, Hinchado MD, Garcia JJ, Ortega E: Influence of gender and oral contraceptives intake on innate and inflammatory response. Role of neuroendocrine factors. Mol Cell Biochem 2008;313:147–153.
  7. Astrand PO, Ryhming I: A nomogram for calculation of the aerobic capacity (physical fitness) from pulse rates during submaximal work. J Appl Physiol 1954;7:218–221.
  8. Boyden S: The chemotaxic effect of mixtures of antibody and antigen on polymorphonuclear leukocyte. J Exp Med 1962;115:453–456.
  9. Ortega E, Forner MA, Barriga C: Exercise-induced stimulation of murine macrophage chemotaxis: role of corticosterone and prolactin as mediators. J Physiol 1997;498:729–734.
  10. Coelho AL, De Freitas MS, Mariano-Oliveira A, Oliveira-Carvalho AL, Zingali RB, Barja-Fidalgo C: Interaction of disintegrins with human neutrophils induces cytoskeleton reorganization, focal adhesion kinase activation, and extracellular-regulated kinase-2 nuclear translocation, interfering with the chemotactic function.FASEB J 2001;15:1643–1645.
  11. Ortega E, Collazos ME, Maynar M, Barriga C, De la Fuente M: Stimulation of the phagocytic function of neutrophils in sedentary men alter acute moderate exercise. Eur J Appl Physiol Occup Physiol 1993;66:60–64.
  12. Rodríguez AB, Barriga C, De la Fuente M: Phagocytic function of blood neutrophils in sedentary young people after physical exercise. Int J Sports Med 1991;12:276–280.
  13. Suzuki K, Naganuma S, Totsuka M, Suzuki KJ, Mochizuki M, Shiraishi M, Nakaji S, Sugawara K: Effects of exhaustive endurance exercise and its one-week daily repetition on neutrophil count and functional status in untrained men. Int J Sports Med 1996;17:205–212.
  14. Ortega E, Barriga C, de la Fuente M: Study of the phagocytic process in neutrophils from elite sports women. Eur J Appl Physiol 1993;66:37–42.
  15. Blanin AK, Chatwin LJ, Cave R, Gleeson M: Effects of submaximal cycling and long-term endurance training on neutrophil phagocytic activity in middle aged men. Br J Sports Med 1996;30:125–129.
  16. Marchena JM, Rodríguez AB, Bariga C, Ortega E: Effect of moderate exercise on the functional capacity of neutrophils of sedentary individuals. J Physiol 2002;543:11–12.
  17. Eberhand A: Physical Exercise and Immunity. Studies and Monographs. Warsaw, Academy of Physical Education, 1982, p 47.
  18. Wolach B, Eliakim A, Gavrieli R, Kodesh E, Yarom Y, Schlesinger M, Falk B: Aspects of leukocyte function and the complement system following aerobic exercise in young female gymnasts. Scand J Med Sci Sports 1998;8:91–97.
  19. Tiidus PM, Bombardier E: Oestrogen attenuates post-exercise myeloperoxidase activity in skeletal muscle of male rats. Acta Physiol Scand 1999;166:85–90.
  20. Azenabor AA, Hoffman-Goetz L: Effect of exhaustive exercise on membrane estradiol concentration intracellular calcium, and oxidative damage in mouse lymphocytes. Free Radic Biol Med 2000;28:84–90.
  21. Ortega E, Giraldo E, Hinchado MD, Martinez M, Ibañez S, Cidoncha A, Collazos ME, Garcia JJ: Role of Hsp72 and norepinephrine in the moderate exercise-induced stimulation of neutrophils’ microbicide capacity. Eur J Appl Physiol 2006;98:250–255.
  22. Ortega E, Marchena JM, Garcia JJ, Barriga C, Rodriguez AB: Norepinephrine as mediator in the stimulation of phagocytosis induced by moderate exercise. Eur J Appl Physiol 2005;93:714–718.
  23. McCarthy DA, Dale MM: The leukocytosis of exercise. Sports Med 1988;6:333–363.
  24. Ostrowski K, Rohde T, Asp S, Schjerling P, Pedersen BK: The cytokine balance and strenuous exercise: TNF-alpha, IL-1beta, IL-6, IL-1ra, sTNF-r1, sTNF-r2 and IL-10. J Physiol 1999;515:287–291.
  25. Jara LJ, Navarro C, Medina G, Vera-Lastra O, Blanca F: Immune-neuroendocrine interactions and autoimmune diseases. Clin Dev Immunol 2006;13:109–123.
  26. McCruden AB, Stimson WH: Sex hormones and immune function; in Ader R, Felten D, Cohen N (eds): Psychoneuroimmunology, ed 2. London, Academic Press, 1991, pp 475–493.
  27. Hardy DB, Janowski BA, Chen CC, Mendelson CR: Progesterone receptor inhibits aromatase and inflammatory response pathways in breast cancer cells via ligand- dependent and ligand-independent mechanisms. Mol Endocrinol 2008;22:1812–1824.
  28. Singh A, Papanicolaou DA, Lawrence LL, Howell EA, Chrousos GP: Neuroendocrine responses to running in women after zinc and vitamin E supplementation. Med Sci Sports Exerc 1999;31:536–542.
  29. Mazzeo R, Donovan SD, Fleshner M, Butterfield GE, Zamudio S, Woffel EE, Moore LG: IL-6 response to exercise and high-altitude exposure: influence of alpha-adrenergic blockade. J Appl Physiol 2001;91:2143–2149.
  30. Suzuki K, Nakaji S, Yamada M, Totsuka M, Sato K, Sugawara K: Systemic inflammatory response to exhaustive exercise. Cytokine kinetics. Exerc Immunol Rev 2002;8:6–48.
  31. Moldoveanu A, Shepard RJ, Shek PN: Exercise elevates plasma levels but not gene expression of IL-1β, IL-6 and TNF-α in blood mononuclear cells. J Appl Physiol 2000;89:1499–1504.
  32. Mastorakos G, Paulatov M, Diamanti-Kadarakis E, Chrousos GP: Exercise and stress system. Hormones 2005;4:73–89.

    External Resources

  33. Derijk RH, Boelen A, Tilders FJH, Berkenbosch F: Induction of plasma interleukin-6 by circulating epinephrine in the rat. Psychoneuroendocrinology 1999;19:155–163.
  34. Espersen GT, Elbaek A, Ernst E, Toft E, Kaalund S, Jersild C, Grunnet N: Effect of physical exercise on cytokines and lymphocyte subpopulations in human peripheral blood. APMIS 1990;98:395–400.


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