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Vol. 42, No. 2, 2009
Issue release date: September 2009
Ophthalmic Res 2009;42:106–111

Cell-Cell Adhesion in the Prelaminar Region of the Optic Nerve Head: A Possible Target for Ionic Stress

Carreras F.J. · Porcel D. · Alaminos M. · Garzón I.
Departments of aSurgery (Ophthalmology) and bCellular Biology, Faculty of Medicine, and cCenter of Scientific Instrumentation, University of Granada, Granada, Spain

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Purpose: This paper aims to study the anterior surface of the optic nerve in relation to its ability to support a source of stress acting from the vitreous cavity. The intercellular junctions of the lining astrocytes mediated by cellular adhesion molecules (CAMs) may be the main targets for ionic stress. Methods: The optic nerve of the domestic pig was prepared for light, confocal laser and transmission electron microscopy. Immunostaining was performed for antibodies against glial fibrillary acidic protein, neural cadherin (N-cadherin) and neural CAM (N-CAM). Results: Only 1 type of intercellular junction was found among the bordering astrocytes, which was characterized as a zonula adherens. Unions between lining cells showed a positive immunogold effect and immunofluorescence against N-cadherin in the zonula adherens and membrane apposition. N-CAM was also present in areas of nonjunctional cellular adhesion. Conclusion: The stability of intercellular junctions of the nerve-vitreous boundary is sensitive to altered concentrations of Ca2+. Since aqueous humor has half the Ca2+ concentration of plasma, any contact of aqueous humor with the optic nerve head can interfere with the ionic concentration of calcium in the extracellular spaces. This mechanism may contribute to age-related changes and some types of glaucoma.

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  1. Hernandez MR: The optic nerve head in glaucoma: role of astrocytes in tissue remodeling. Prog Retin Eye Res 2000;19:297–321.
  2. Liu B, Neufeld AN: Nitric oxide synthase-2 in human optic nerve head astrocytes induced by elevated pressure in vitro. Arch Ophthalmol 2001;119:240–245.
  3. Anderson DR, Hoyt WF: Ultrastructure of intraorbital portion of human and monkey optic nerve. Arch Ophthalmol 1970;82:506–530.

    External Resources

  4. Anderson DA: Ultrastructure of human and monkey lamina cribrosa and optic nerve head. Arch Ophthalmol 1969;82:800–814.
  5. Ye H, Hernandez MR: Heterogeneity of astrocytes in human optic nerve head. J Comp Neurol 1995;362:441–452.
  6. Anderson DR: Ultrastructure of the optic nerve head. Arch Opthalmol 1970;83:63–73.
  7. Kobayashi S, Vidal I, Pena JD, Hernandez MR: Expression of neural cell adhesion molecule (NCAM) characterizes a subpopulation of type 1 astrocytes in human optic nerve head. Glia 1997;20:262–273.
  8. Takeichi M, Yoshida-Noro C, Shirayoshi Y, Hatta K: Calcium dependent cell-cell adhesion system: its molecular nature, celltype specificity and morphogenetic role; in Edelman GM, Thiery JP (eds): The Cell in Contact: Adhesions and Junctions as Morphogenetic Determinants. New York, Wiley, 1985, pp 219–232.
  9. Lin HJ, Tsai FJ, Hung P, Chen WC, Chen HY, Fan SS, Tsai SW: Association of E-cadherin gene 3′-UTR C/T polymorphism with primary open angle glaucoma. Ophthalmic Res 2006;38:44–48.
  10. Kypta R, Bernfield M, Burridge K: Cell junctions, cell adhesion, and the extracellular matrix; in Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P (eds): Molecular Biology of the Cell, ed 4. New York, Garland Publishing, 2002, chapt 19.
  11. Tacheichi M, Hatta K, Nagafuchi A: Identification of a gene family of cadherin cell adhesion molecules. Cell Differ Dev 1988;25(suppl):91–94.
  12. Brusés JL: N-cadherin signaling in synapse formation and neuronal physiology. Mol Neurobiol 2006;33:237–252.
  13. Rosen CL, Bunge RP, Ard MD, Wood PM: Type 1 astrocytes inhibit myelination by adult rat oligodendrocytes in vitro. J Neurosci 1989;9:3371–3379.
  14. Frenzel EM, Johnson RG: Gap junction formation between cultured embryonic lens cells is inhibited by antibody to N-cadherin. Dev Biol 1996;179:1–16.
  15. Makrigiannakis A, Coukis G, Blaschuk O, Coutifaris C: Follicular atresia and luteolysis: evidence for a role for N-cadherin. Ann NY Acad Sci 2000;900:46–55.
  16. Cole DF: Comparative aspects of the intraocular fluids; in Davson H, Graham LT (eds): The Eye, ed 2. New York, Academic Press, 1974, vol 5: Comparative Physiology, pp 71–161.
  17. Bito LZ: Intraocular fluid dynamics. I. Steady-state concentration gradients of magnesium, potassium and calcium in relation to the sites and mechanisms of ocular cation transport processes. Exp Eye Res 1970;10:102–116.
  18. Whitmore AV, Libby RT, John SWM: Glaucoma: thinking in new ways – a rôle for autonomous axonal self-destruction and other compartmentalised processes? Prog Retin Eye Res 2005;24:639–662.

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