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Vol. 54, No. 4, 2009
Issue release date: October 2009
Section title: Original Paper
Ann Nutr Metab 2009;54:253–257
(DOI:10.1159/000229505)

Characterization of Bacteria, Clostridia and Bacteroides in Faeces of Vegetarians Using qPCR and PCR-DGGE Fingerprinting

Liszt K. · Zwielehner J. · Handschur M. · Hippe B. · Thaler R. · Haslberger A.G.
Department of Nutritional Sciences, University of Vienna, Vienna, Austria

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 9/22/2008
Accepted: 5/5/2009
Published online: 7/27/2009

Number of Print Pages: 5
Number of Figures: 2
Number of Tables: 0

ISSN: 0250-6807 (Print)
eISSN: 1421-9697 (Online)

For additional information: http://www.karger.com/ANM

Abstract

Background/Aims: This study aimed to investigate the quantitative and qualitative changes of bacteria, Bacteroides, Bifidobacterium and Clostridium cluster IV in faecal microbiota associated with a vegetarian diet. Methods: Bacterial abundances were measured in faecal samples of 15 vegetarians and 14 omnivores using quantitative PCR. Diversity was assessed with PCR-DGGE fingerprinting, principal component analysis (PCA) and Shannon diversity index. Results: Vegetarians had a 12% higher abundance of bacterial DNA than omnivores, a tendency for less Clostridium cluster IV (31.86 ± 17.00%; 36.64 ± 14.22%) and higher abundance of Bacteroides (23.93 ± 10.35%; 21.26 ± 8.05%), which were not significant due to high interindividual variations. PCA suggested a grouping of bacteria and members of Clostridium cluster IV. Two bands appeared significantly more frequently in omnivores than in vegetarians (p < 0.005 and p < 0.022). One was identified as Faecalibacterium sp. and the other was 97.9% similar to the uncultured gut bacteriumDQ793301. Conclusions: A vegetarian diet affects the intestinal microbiota, especially by decreasing the amount and changing the diversity of Clostridium cluster IV. It remains to be determined how these shifts might affect the host metabolism and disease risks.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 9/22/2008
Accepted: 5/5/2009
Published online: 7/27/2009

Number of Print Pages: 5
Number of Figures: 2
Number of Tables: 0

ISSN: 0250-6807 (Print)
eISSN: 1421-9697 (Online)

For additional information: http://www.karger.com/ANM


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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