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Vol. 54, No. 4, 2009
Issue release date: October 2009
Ann Nutr Metab 2009;54:268–274

Relationship between Increased Body Iron Stores, Oxidative Stress and Insulin Resistance in Healthy Men

Syrovatka P. · Kraml P. · Potockova J. · Fialova L. · Vejrazka M. · Crkovska J. · Andel M.
aDepartment of Cardiology, Institute for Clinical and Experimental Medicine, bSecond Department of Internal Medicine, Third Faculty of Medicine, and cFirst Department of Medical Chemistry, First Faculty of Medicine, Charles University, Prague, Czech Republic

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Aim: The aim of our cross-sectional study was to assess the relationships between body iron stores, oxidative stress, impaired insulin sensitivity and carotid atherosclerosis in a cohort of healthy men in primary prevention of cardiovascular disease. Methods: We examined 151 volunteers, aged 35– 60 years. Anthropometric parameters, markers of metabolic syndrome, insulin resistance, inflammatory markers, parameters of oxidative stress and intima-media thickness of common carotid artery were measured. Results: Ferritin correlated positively with waist circumference, body mass index, impaired insulin sensitivity, plasma triglycerides and inversely with high-density lipoprotein cholesterol. We observed positive correlations between ferritin, oxidized lowdensity lipoprotein and advanced oxidation protein products after adjustment for age, waist circumference, body mass index and measured inflammatory markers (high-sensitivity C-reactive protein, fibrinogen, interleukin-6 and tumor necrosis factor-α). There were no significant associations between ferritin and intima-media thickness or markers of endothelial dysfunction. In a stepwise multiple regression analysis, triglycerides, waist circumference and elevated transaminases were independent determinants of the serum ferritin level. Conclusion: Our results provide evidence for a relationship between plasma ferritin and oxidative modification of lipids as well as proteins in vivo. Higher body iron stores may contribute to impaired insulin sensitivity through increased oxidative stress in a cohort of healthy men.

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  1. You SA, Wang Q: Ferritin in atherosclerosis. Clin Chim Acta 2005;357:1–16.
  2. Swaminathan S, Fonseca VA, Alam MG, Shah SV: The role of iron in diabetes and its complications. Diabetes Care 2007;7:1926–1933.

    External Resources

  3. Sullivan JL: Iron and the sex difference in heart disease risk. Lancet 1981;i:1293–1294.
  4. Salonen JT, Nyyssönen K, Korpela H, Tuomilehto J, Seppänen R, Salonen R: High stored iron levels are associated with excess risk of myocardial infarction in eastern Finnish men. Circulation 1992;86:803–811.
  5. Kiechl S, Willeit J, Egger G, Poewe W, Oberhollenzer F: Body iron stores and the risk of carotid atherosclerosis: prospective results from the Bruneck study. Circulation 1997;96:3300–3307.
  6. Tuomainen TP, Salonen R, Nyyssönen K, Salonen JT: Cohort study of relation between donating blood and risk of myocardial infarction in 2,682 men in eastern Finland. BMJ 1997;314:793–794.
  7. Araujo JA, Romano EL, Brito BE, Parthé V, Romano M, Bracho M, Montaño RF, Cardier J: Iron overload augments the development of atherosclerotic lesions in rabbits. Arterioscler Thromb Vasc Biol 1995;15:1172–1180.
  8. Ishizaka N, Saito K, Mitani H, Yamazaki I, Sata M, Usui S, Mori I, Ohno M, Nagai R: Iron overload augments angiotensin II-induced cardiac fibrosis and promotes neointima formation. Circulation 2002;106:1840–1846.
  9. Kartikasari AE, Georgiou NA , Visseren FL, van Kats-Renaud H, van Asbeck BS, Marx JJ: Endothelial activation and induction of monocyte adhesion by nontransferrin-bound iron present in human sera. FASEB J 2006;20:353–355.
  10. Stampfer MJ, Grodstein F, Rosenberg I, Willett W, Hennekens C: A prospective study of plasma ferritin and risk of myocardial infarction in US physicians. Circulation 1993;87:688–688.

    External Resources

  11. Ascherio A, Willet WC, Rimm EB, Giovannucci EL, Stampfer MJ: Dietary iron intake and risk of coronary disease among men. Circulation 1994;89:969–974.
  12. Moore M, Folsom AR, Barnes RW, Eckfeldt JH: No association between serum ferritin and asymptomatic carotid atherosclerosis. The Atherosclerosis Risk in Communities (ARIC) Study. Am J Epidemiol 1995;141:719–723.
  13. Levy JC, Matthews DR, Hermans MP: Correct homeostasis model assessment (HOMA) evaluation uses the computer program. Diabetes Care 1998;21:2191–2192.
  14. Witko-Sarsat V, Friedlander M, Capeillere-Blandin CH, Nguyen-Khoa T, Nguyen AT, Zingraff J, et al: Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int 1996;49:1304–1313.
  15. Kyriakis JM, Avruch J: Sounding the alarm: protein kinase cascades activated by stress and inflammation. J Biol Chem 1996;271:24313–24316.
  16. Paz K, Hemi R, LeRoith D, Karasik A, Elhanany E, Kanety H, et al: A molecular basis for insulin resistance: elevated serine/threonine phosphorylation of IRS-1 and IRS-2 inhibits their binding to the juxtamembrane region of the insulin receptor and impairs their ability to undergo insulin-induced tyrosine phosphorylation. J Biol Chem 1997;272:29911–29918.
  17. Evans JL, Goldfine ID, Maddux BA, Grodsky GM: Are oxidative stress-activated signaling pathways mediators of insulin resistance and β-cell dysfunction? Diabetes 2003;52:1–8.
  18. Tuomainen NP, Nyyssönen K, Salonen R, Tervahauta A, Korpela H, Lakka T, et al: Body iron stores are associated with serum insulin and blood glucose concentrations. Population study in 1,013 eastern Finnish men. Diabetes Care 1997;20:426–428.
  19. Fernández-Real JM, Ricart-Engel W, Arroyo E, Balançá R, Casamitjana-Abella R, Cabrero D, et al: Serum ferritin as a component of the insulin resistance syndrome. Diabetes Care 1998;21:62–68.
  20. Ford ES, Cogswell ME: Diabetes and serum ferritin concentration among US adults. Diabetes Care 1999;22:1978–1983.
  21. Mendler MH, Turlin B, Moirand R, Jouanolle AM, Sapey T, Guyader D, et al: Insulin resistance-associated hepatic iron overload. Gastroenterology 1999;117:1155–1163.
  22. Trombini P, Piperno A: Ferritin, metabolic syndrome and NAFLD: elective attractions and dangerous liaisons. J Hepatol 2007;46:549–552.
  23. Chitturi S, Farrell GC: Etiopathogenesis of nonalcoholic steatohepatitis. Semin Liver Dis 2001;21:27–41.
  24. Zacharski LR, Chow BK, Howes PS, Shamayeva G, Baron JA, Dalman RL, et al: Reduction of iron stores and cardiovascular outcomes in patients with peripheral arterial disease. JAMA 2007;297:603–610.
  25. Facchini FS: Effect of phlebotomy on plasma glucose and insulin concentrations. Diabetes Care 1998;21:2190.
  26. Fernández-Real JM, López-Bermejo A, Ricart W: Iron stores, blood donation and insulin sensitivity and secretion. Clin Chem 2005;51:1201–1205.
  27. Conrad ME, Umbreit JN: Iron absorption: relative importance of iron transport pathways. Am J Hematol 2001;67:215.
  28. Wang Y, Beydoun MA: Meat consumption is associated with obesity and central obesity among US adults. Int J Obes 2009;24:1–8.
  29. Harrison EH: Mechanisms of digestion and absorption of dietary vitamin A. Annu Rev Nutr 2005;25:87–103.
  30. Beard J, Tobin B: Iron status and exercise. Am J Clin Nutr 2000;72:594–597.
  31. Lutsey PL, Steffen LM, Stevens J: Dietary intake and the development of the metabolic syndrome: the Atherosclerosis Risk in Communities study. Circulation 2008;117:754–761.
  32. Slentz CA, Aiken LB, Houmard JA, Bales CW, Johnson JL, Tanner CJ, Duscha BD, et al: Inactivity, exercise, and visceral fat. STRRIDE: a randomized, controlled study of exercise intensity and amount. J Appl Physiol 2005;99:1613–1618.

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