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Vol. 108, No. 1, 1995
Issue release date: 1995
Int Arch Allergy Immunol 1995;108:49–54

Characterization of the Allergen Group VI in Timothy Grass Pollen (PhI p 6) I. Immunological and Biochemical Studies

Petersen A. · Bufe A. · Schlaak M. · Becker W.-M.
Division of Allergology, Forschungsinstitut Borstel, Germany

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For the identification and characterization of allergen grass group VI we performed two-dimensional immunoblotting of timothy grass pollen (Phleum pratense). Two intense 13-kD protein spots of pi 5.2 and 5.5 were found to be IgE reactive. By N-terminal microsequencing and amino acid analysis we identified them as Phl p 6 isoallergens. An antiserum was raised against Phl p 6 by immunizing BALB/c mice with allergen bearing nitrocellulose particles of one isoform. The antiserum revealed an intense reactivity to Phi p 6 isoforms, but also showed a weak cross-reactivity with Phl p 5 allergens. After immunoabsorption of patients’ serum to Phl p 6 spots on the blotting membrane, we were able to demonstrate that the eluted human IgE antibodies cross-react with the grass group V allergens as well. Therefore, Phl p 5 and Phl p 6 possess one or more common IgE binding epitopes.

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