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Table of Contents
Vol. 6, No. 2, 1999
Issue release date: March–April 1999
Section title: Original Paper
J Biomed Sci 1999;6:115–120
(DOI:10.1159/000025378)

Isolation of Full-Length cDNA and Chromosomal Localization of Human NF-κB Modulator NEMO to Xq28

Jin D.-Y. · Jeang K.-T.
Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Md., USA

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 3/19/1999

Number of Print Pages: 6
Number of Figures: 5
Number of Tables: 0

ISSN: 1021-7770 (Print)
eISSN: 1423-0127 (Online)

For additional information: http://www.karger.com/JBS

Abstract

NEMO is an essential component of the IκB kinase complex. Others have shown that expression of mouse NEMO can complement the lack of responsiveness to NF-κB stimuli in two NEMO-deficient cell lines. Here we report the isolation of a full-length human NEMO cDNA. Virtual translation of human NEMO cDNA predicts a 48-kD coiled-coil protein which shares 87.9% identity and 90.5% similarity with the mouse homolog. By sequence alignment, we mapped the human NEMO gene to chromosome Xq28. We note that the NEMO and the G6PD (glucose-6-phosphate dehydrogenase) loci are arranged in a head-to-head orientation separated by no more than 800 bp. This map location is further supported by the sequence of an alternatively spliced variant of human NEMO mRNA. Thus, human NEMO is an X-linked gene closely adjacent to the G6PD locus.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Published online: 3/19/1999

Number of Print Pages: 6
Number of Figures: 5
Number of Tables: 0

ISSN: 1021-7770 (Print)
eISSN: 1423-0127 (Online)

For additional information: http://www.karger.com/JBS


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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