Thrombin Peptide TP508 Stimulates Rapid Nitric Oxide Production in Human Endothelial CellsOlszewska-Pazdrak B. · Hart-VanTassell A. · Carney D.H.
Therapeutic Peptide Development Laboratory, Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Tex., USA Corresponding Author
TP508, a 23-amino-acid peptide representing a portion of human thrombin, promotes tissue revascularization and repair. The molecular mechanisms of TP508 action, however, remain unclear. Nitric oxide (NO) plays a crucial role in regulation of angiogenesis and wound healing. We, therefore, investigated TP508 effects on NO production in human endothelial cells. TP508 stimulated a rapid, dose-dependent, 2- to 4-fold increase in NO production. TP508 induced NO release as early as 5 min. Continued exposure to TP508 for 1–24 h increased NO concentrations over controls by 100.5 ± 9.6 and 463.3 ± 24.2 nM, respectively. These levels of NO release were similar to those produced in response to vascular endothelial growth factor (VEGF). TP508- and VEGF-induced NO production was decreased by inhibitors of PI-3K (LY294002) and Src (PP2). TP508 stimulated early transient phosphorylation of Src and Akt. In contrast to VEGF, TP508 stimulation of NO release was inhibited by PKC inhibitor (Go6976) and was independent of intracellular calcium mobilization. These results demonstrate that TP508 and VEGF stimulate NO production to similar levels but through distinct pathways. This study provides new insights into the initial molecular mechanisms by which TP508 may stimulate diverse cellular effects leading to tissue revascularization and wound healing.
Copyright © 2009 S. Karger AG, Basel
TP508 is a 23-amino-acid synthetic peptide representing residues 508–530 of human prothrombin. This molecule was originally identified as the receptor-binding domain of α-thrombin based on competition for high-affinity α-thrombin binding to fibroblasts and enhancement of fibroblast proliferation . In contrast to thrombin, TP508 has no enzymatic activity and does not promote or interfere with blood coagulation . Thus, TP508 has been used to study nonproteolytic effects of thrombin on cells and in various animal models [2,3,4,5]. These studies indicate that TP508 exerts its cellular effects by activation of a receptor on cells distinct from the protease-activated receptors (PAR1–4) .
Preclinical studies have shown that TP508 accelerates healing of normal dermal full-thickness wounds [7,8], ischemic dermal wounds , fractures in rats , segmental bone defects , distraction osteogenesis defects  and high-impact fractures involving bone and soft tissue . Based on these results, TP508 is now being evaluated in clinical trials for treatment of diabetic foot ulcers [14,15] and repair of distal radius fractures [15,16,17].
Effective wound repair requires growth factor-stimulated angiogenesis . Angiogenesis, the process of new blood vessel formation from preexisting ones, is comprised of several discrete steps including dissociation of matrix, endothelial cell migration, proliferation and tube differentiation . TP508 stimulated early revascularization in dermal and bone injury models [7,8,10,12,13,20], and stimulated vascular sprouting in ex vivo cultures of microvessel fragments . Thus, TP508 effects on tissue repair appear to be linked to its stimulation of angiogenesis.
Nitric oxide (NO) has been shown to be a crucial signaling molecule in the regulation of angiogenesis. Endothelium-derived NO produced by endothelial NO synthase (eNOS) plays a significant role in vascular endothelial growth factor (VEGF)-induced angiogenesis in vivo  and in vitro [23,24]. In addition, angiogenesis was significantly attenuated in eNOS-deficient mice and was not improved by administration of VEGF, suggesting that both eNOS activation and NO production are required for VEGF-stimulated angiogenesis .
Other studies have demonstrated the involvement of NO in wound healing [26,27]. For example, eNOS-deficient mice show impaired wound healing , while NO produced locally using NO donor molecules or eNOS gene therapy accelerates tissue repair [29,30]. In addition, treatment of diabetic wounds with L-arginine to increase NO production enhances healing both in animal models  and in diabetic foot ulcers .
We recently reported that in a pig model of chronic myocardial ischemia, TP508 restored myocardial function, endothelial responsiveness to NO-dependent vasodilators and microvessel NO production . In addition, long-term (24 h) treatment of human coronary artery endothelial cells (HCAECs) with TP508 also increased NO production . These studies show long-term effects of TP508 that may involve the induction of VEGF or other angiogenic cytokines. More recently, systemic infusion of TP508 was shown to decrease infarct size, improve NO-dependent microvascular function, and decrease apoptosis in normal and hypercholesterolemic pig models of acute myocardial ischemia-reperfusion injury [34,35]. Because NO has been reported to have cardioprotective effects , these effects of TP508 could also involve NO production.
To understand the initial molecular mechanisms by which TP508 promotes angiogenesis, tissue repair and myocardial protection, we have examined the direct effects of TP508 on early NO production in human endothelial cells. Our results demonstrate that TP508 stimulates rapid NO production in endothelial cells and that the levels of TP508-stimulated NO production are similar to the levels of NO produced in response to VEGF. Results also show that TP508 and VEGF stimulate NO production by distinct signaling pathways. In contrast to previous studies, these results demonstrate direct early effects of TP508 on human endothelial cells and provide new insights into the mechanism by which TP508 stimulates tissue repair.
Materials and Methods
Thrombin peptide TP508 (AGYKPDEGKRGDACEGDSGGPFV, rusalatide acetate or Chrysalin®, CAS No. 87455-82-6) was synthesized and purified by American Peptide Company (Sunnyvale, Calif., USA). VEGF165 was purchased from R&D System (Minneapolis, Minn., USA). Calcium ionophore A23187 and kinase inhibitors LY294002, PP2, PD98059 and Go6976 were purchased from Calbiochem (San Diego, Calif., USA). N-nitro-L-arginine methyl ester (L-NAME), L-arginine, NaNO2 and NaI were purchased from Sigma (St. Louis, Mo., USA).
HCAECs and bovine aortic endothelial cells (BAECs) were purchased from Lonza (Walkersville, Md., USA). Human umbilical vein endothelial cells (HUVECs) were purchased from BD Bioscience (San Jose, Calif., USA). Cells were cultured in endothelial growth medium (EGM) which is endothelial cell basal medium (EBM) supplemented with 5% fetal bovine serum and growth factors SingleQuots™ (Clonetics, San Diego, Calif., USA) in 5% CO2 at 37°C. Cells between passages 4 and 7 were used for the experiments. HCAECs obtained from two different donors were tested in these studies.
Cells were plated in 24-well plates at a density of 50,000/well and grown in EGM until 2 days after confluency. The medium was replaced with EBM containing 200 µML-arginine and cells were stimulated with TP508 (20 µM) or VEGF (2.5 nM) or calcium ionophore A23187 (1 µM) for 1 h or indicated times. In some experiments, cells were pretreated with the specific kinase inhibitors L-NAME (100 µM), LY294002 (50 µM), PP2 (10 µM), PD98059 (50 µM), or Go6976 (100 nM) for 45 min before stimulation. Inhibitor concentrations used have previously shown selective kinase inhibition in endothelial cells [23,37,38]. Supernatants were collected and analyzed for total nitrite, a stable breakdown product of NO in aqueous solutions, using a chemiluminescence NO analyzer (Model 270B; Sievers Instruments, Boulder, Colo., USA) as previously described . Briefly, 100 µl of sample was placed in a reaction chamber containing a mixture of sodium iodide in glacial acetic acid to reduce nitrite to NO and allow the liberation of NO gas from the aqueous mixture. The analyzer was calibrated for each experiment using a sodium nitrite standard curve. NO concentration in the EBM containing L-arginine was used as a background value and was subtracted from sample values to determine the amount of NO released from cells.
Two-day postconfluent cultures of HCAECs were treated with TP508 (20 µM) for indicated times. Cells were solubilized in lysis buffer (150 mM NaCl, 50 mM HEPES, pH 7.4, 1 mM EGTA, 1 mM Na3VO4, 10 mM NaF, 1 mM phenylmethylsulfonyl fluoride, 5% glycerol, 1% Triton X-100) containing a protease inhibitor mixture (Complete, Roche Diagnostics, Indianapolis, Ind., USA). Cell lysates were subjected to SDS-PAGE and transferred to nitrocellulose membranes (0.2 µm, Invitrogen, Carlsbad, Calif., USA). Membranes were incubated overnight at 4°C with primary antibodies against phospho-Src family (Y416) and phospho-Akt (S473) (Cell Signaling Technology, Beverly, Mass., USA). After stripping, blots were reprobed with Src or Akt antibodies (Cell Signaling Technology). Immunoblots were developed using Immobilon Western Detection Reagents (Millipore Corporation, Billerica, Mass., USA).
Changes in intracellular calcium levels were determined us- ing the Fluo-4 NW Calcium Assay Kit (Molecular Probes, Eugene, Oreg., USA) according to the manufacturer’s instructions. HCAECs were grown on 96-well plates until 2 days after confluency in EGM. Cells were incubated with 100 µl of the Fluo-4 NW dye loading solution at 37°C for 30 min, then at room temperature for an additional 30 min. Cells were stimulated with TP508 (20 µM) or VEGF (2.5 nM) or calcium ionophore A23187 (1 µM), and fluorescence intensity was measured at indicated times using a Synergy HT fluorescence reader (BioTek, Winooski, Vt., USA) with excitation at 494 nm and emission at 516 nm. Baseline fluorescence measured in control nonstimulated cells was subtracted from the fluorescence values obtained after the indicated treatments. Each experiment was performed in triplicate.
Data are presented as means ± standard deviation. Statistical analysis was performed using Student’s t test. p < 0.05 was considered as statistically significant.
To determine initial mechanisms by which TP508 promotes angiogenesis and tissue protection, we examined the effect of TP508 on early NO production in endothelial cells. Stimulation of HCAECs with TP508 for 1 h resulted in an ∼2.5-fold increase in the level of NO in supernatants compared to control cells (153.5 ± 15.3 vs. 65.3 ± 7.0 nM) (fig. 1a). This effect was not limited to HCAECs. Stimulation of HUVECs and BAECs with TP508 also increased NO production ∼4-fold and ∼3-fold compared to control cells, respectively (fig. 1a). Interestingly, the levels of NO production stimulated by TP508 were comparable to the levels of VEGF-induced NO release from these same cells (fig. 1a). Pretreatment of HCAECs with the NO synthase (NOS) inhibitor, L-NAME effectively blocked TP508-stimulated NO production (fig. 1b). Thus, confirming that the increased levels of NO production, as measured from total nitrite accumulation in the medium, resulted from activation of NOS.
|Fig. 1. TP508 stimulates rapid NO production in different primary endothelial cells. a HCAECs, HUVECs and BAECs were stimulated with TP508 (20 µM) or VEGF (2.5 nM) for 1 h. NO released into supernatants was determined by NO analyzer. a p < 0.01 compared to control (n = 4). b Effect of NO synthase inhibitor L-NAME on TP508-induced NO production. HCAECs were pretreated with L-NAME (100 µM) for 45 min before stimulation with TP508 (20 µM) for 1 h. NO released into the supernatants was determined by NO analyzer. a p < 0.01 compared to control; b p <0.01 compared to TP508 (n = 4).|
TP508 induced NO production by endothelial cells in a dose-dependent manner in both HCAECs and BAECs (fig. 2). Stimulation of cells with 0.4 µM TP508 was sufficient to significantly increase NO production in both HCAECs (135.3 ± 16.2 vs. 74.1 ± 12.4 nM) and in BAECs (62.0 ± 8.8 vs. 24.1 ± 12.2 nM) compared to control cells. The maximal level of NO production was obtained from cells stimulated with 20–40 µM TP508. There was no significant difference between doses of 20–40 µM TP508 in human (317.75 ± 32.2 vs. 322.4 ± 25.2 nM) and bovine (224.0 ± 20.6 vs. 234.4 ± 26.8 nM) endothelial cells (fig. 2). Thus, 20 µM TP508 (∼50 µg/ml) was considered the optimal dose for these cells and has been used throughout these experiments. We also determined that 2.5 nM (∼50 ng/ml) was the optimal dose for VEGF-stimulated production of NO in these same cells (data not shown).
|Fig. 2. Dose response of TP508-stimulated NO production. HCAECs (a) and BAECs (b) were incubated with the indicated doses of TP508 for 1 h. NO released into supernatants was determined by NO analyzer. Data are presented as means ± SD (n = 4).|
Our studies showed that TP508 stimulated rapid NO release from endothelial cells within 5–10 min, reached a maximum level over controls by 15 min and then remained approximately the same during a 1-hour time course (fig. 3a). This rapid stimulation suggests activation of constitutively expressed eNOS. Continued exposure of cells to TP508 for 1, 3, 6, 12 and 24 h resulted in increased NO concentrations (over control levels for each time point) by 100.5 ± 9.0, 214.8 ± 37.8, 394.5 ± 45.5, 466.5 ± 16.7 and 463.3 ± 24.2 nM (fig. 3b). The levels of VEGF-stimulated NO production over this time course were similar to those seen with TP508 at 1 and 24 h (fig. 3b). Interestingly, at intermediate time points between 3 and 12 h, TP508-stimulated NO production was significantly higher than VEGF-stimulated NO production. In contrast, stimulation with calcium ionophore resulted in much higher NO production at all time points tested compared to TP508 or VEGF, reaching a maximum of 2,264 ± 79 nM at 24 h (fig. 3b).
|Fig. 3. Time course of TP508-stimulated NO production. a BAECs were treated with or without TP508 (20 µM) for 5, 15, 30 and 60 min. After the indicated times, the supernatants were collected from control and TP508-stimulated cells, and NO released into the supernatants was determined by NO analyzer. a p < 0.01, compared to control (n = 4). b BAEC were incubated with or without TP508 (20 µM), VEGF (2.5 nM), or calcium ionophore A23187 (1 µM). After 1, 3, 6, 12 and 24 h stimulation, the supernatants were collected and NO concentration in the samples was determined by NO analyzer. Data are presented as NO concentrations over control values for each time point. a p < 0.01, compared to VEGF; b p < 0.01, compared to TP508 or VEGF (n = 4).|
To investigate potential signaling pathways that might be involved in TP508-induced NO production, cells were pretreated with specific kinase inhibitors for 45 min before stimulation. In both HCAECs and BAECs, the amount of TP508-stimulated NO production at 1 h was significantly reduced by inhibitors of PI-3K (LY294002), Src (PP2) and PKC (Go6976) but not by MEK inhibitor (PD98059) (fig. 4a). This suggests the possible involvement of PI-3K, Src and PKC-α/β in TP508-induced activation of eNOS. VEGF- and calcium ionophore A23187-stimulated NO production was also attenuated by inhibitors of PI-3K (LY294002) and Src (PP2) (fig. 4b). In contrast to TP508, however, the Go6976 inhibitor specific for PKC-α/β had no significant effect on VEGF- or calcium ionophore-stimulated NO release (fig. 4b). That the kinase inhibitors showed different patterns of inhibition between TP508-, calcium ionophore- and VEGF-stimulated NO release demonstrates the specificity of these inhibitors. These kinase inhibitor studies suggest that TP508 and VEGF stimulation of NO production may both involve PI-3K and Src pathways, but that TP508 stimulation may also be dependent on PKC-α/β signaling in these cells.
|Fig. 4. Effect of specific kinase inhibitors on TP508-stimulated NO production. a HCAECs and BAECs were preincubated with specific kinase inhibitors LY294002 (LY), PP2 (PP2), PD98059 (PD) and Go6976 (Go) for 45 min before stimulation with TP508 (20 µM) (TP) for 1 h. b BAEC were preincubated with specific kinase inhibitors LY294002 (LY), PP2 (PP2), PD98059 (PD) and Go6976 (Go) for 45 min. Cells were stimulated with or without VEGF (2.5 nM) or calcium ionophore A23187 (1 µM) for 1 h. NO concentration in samples was determined by NO analyzer. a p < 0.01, compared to control (CTR); b p < 0.01, compared to TP or Ca or VEGF without inhibitors (n = 4).|
Previous studies have shown that eNOS activation by VEGF requires intracellular calcium mobilization [37,40], while insulin, estrogen and shear stress do not [41,42,43]. To determine whether changes in intracellular calcium concentrations were involved in TP508-stimulated NO production, we measured intracellular calcium levels using a Fluo-4 fluorescence calcium indicator. TP508 treatment did not significantly affect intracellular calcium concentrations in HCAECs during 10 min of stimulation (fig. 5). Moreover, we did not observe any significant effect of TP508 on calcium levels during incubation up to 1 h (data not shown). In contrast, VEGF and calcium ionophore treatment of these cells resulted in a rapid increase in intracellular calcium levels (fig. 5). These same cells were also tested for NO production in parallel experiments which showed that stimulation of these cells by all three factors resulted in increased NO release. These data indicate that TP508-induced stimulation of NO production does not require intracellular calcium mobilization. This result demonstrates a significant difference between VEGF and TP508 in the activation of eNOS.
|Fig. 5. Effect of TP508 on intracellular calcium concentration. HCAECs were loaded with Fluo-4 NW dye and stimulated with TP508 (20 µM), VEGF (2.5 nM), or calcium ionophore A23187 (1 µM). Changes in intracellular calcium levels were measured by changes in fluorescence intensity of Fluo-4 dye at the indicated times after treatment. Data are presented as relative fluorescence intensity compared to control cells. a p < 0.01, compared to control (n = 3). Arrow indicates time 0, when factors were added to cells.|
Insulin, estrogen and other factors have been reported to stimulate NO production through calcium-independent mechanisms [41,42,43,44]. Calcium-independent eNOS activation appears to involve Src phosphorylation of caveolin-1, phosphorylation of Akt and caveolae internalization [45,46]. TP508-stimulated NO production was decreased by Src and Akt inhibitors. We therefore evaluated TP508 effects on Src and Akt activation. As shown in figure 6, Western blot analyses with phospho-specific antibodies, recognizing the activated form of Src family kinases (Y416) or activated Akt (S473), showed that TP508 caused a transient phosphorylation of Akt and a Src kinase in HCAECs. Together, these results suggest that TP508 stimulates early NO production through a mechanism that is calcium independent, but that involves activation of Src and Akt.
|Fig. 6. TP508 activates Src and Akt kinase. HCAECs were stimulated with TP508 (20 µM) for the indicated times. Activation of Src or Akt was determined by Western blotting using antibodies specific for phospho-Src family (Y416) or phospho-Akt (S473). Immunoblots were reprobed with antibodies recognizing total Src or Akt. Representative experiments are shown (n = 2).|
The present studies were undertaken to determine the direct effect of the nonproteolytic thrombin peptide TP508 on early NO production in primary endothelial cells. TP508 has previously been shown to stimulate tissue revascularization following injury [7,8,10,12,20] and angiogenic sprouting in ex vivo cultures of microvessel fragments . Recent in vivo and long-term culture experiments showed that TP508 reverses endothelial dysfunction and increases NO production in endothelial cells . These studies, however, only investigated long-term effects that may result from TP508-induced upregulation of VEGF or other angiogenic cytokines. It was therefore important to determine whether TP508 directly stimulated early NO production in endothelial cells as a signaling mechanism to promote angiogenesis. Our studies demonstrate for the first time that TP508 stimulates rapid NO release in human endothelial cells. Moreover, the effects of TP508 on NO production in these endothelial cells are comparable to the effects of the well-characterized angiogenic factor VEGF.
NO produced by endothelial cells plays an important role in angiogenesis following injury . Moreover, NO production induced by VEGF also significantly contributes to its growth-promoting effect on endothelial cells [24,47]. We have shown that TP508 accelerates wound healing in animal models and increases revascularization of injured tissues [7,8,9]; however, the molecular mechanisms responsible for accelerated tissue repair and revascularization remain unclear. In this study, we showed that TP508 induced NO release during 1 h treatment in HCAECs, HUVECs and BAECs.
To evaluate the biological significance of these results, we compared the effect of TP508 with the effect of the angiogenic factor VEGF. These studies demonstrated that TP508 stimulated NO production to levels similar to those caused by VEGF stimulation. TP508 stimulated rapid NO release, as early as 5 min after treatment, with continued NO accumulation during exposure up to 24 h. NO levels measured in VEGF-treated cells were similar to NO levels in TP508-treated cells after 1 and 24 h incubation.
As an initial step to explore signaling pathways involved in TP508-induced NO production, we pretreated cells for 45 min with specific kinase inhibitors and measured NO production after 1 h stimulation with TP508, VEGF, or calcium ionophore A23187. We found that TP508-stimulated NO production was significantly decreased by inhibitors of PI-3K (LY294002), PKC (Go6976) and Src (PP2) but not by MEK inhibitor (PD98059). This suggested involvement of PI-3K, PKC-α/β and Src in activation of eNOS by TP508. VEGF- and calcium ionophore-stimulated NO production was attenuated by inhibitors of PI-3K (LY294002) and Src (PP2), but not by the MEK inhibitor (PD98059) or the inhibitor of PKC-α/β (Go6976). Thus, these results suggest that eNOS activation by TP508 involves activation of PKC-α/β, while activation by VEGF or calcium ionophore does not. The lack of inhibition of VEGF stimulation by Go6976 is consistent with a recent study showing that siRNA-mediated downregulation of PKC-ε but not PKC-α abrogated VEGF-stimulated eNOS activity in BAECs . The Src inhibitor (PP2) significantly prevented NO release in response to TP508, calcium ionophore and VEGF. This result is consistent with three potential roles played by Src in the activation of eNOS under different conditions. First, it has been shown that Src-mediated phosphorylation of Hsp90 is required for VEGF-stimulated NO production . Second, tyrosine phosphorylation by Src can activate eNOS . Third, Src phosphorylation of PI-3K and Akt activate eNOS in response to estrogen . The PI-3K inhibitor (LY294002) also significantly prevented NO release in response to TP508, calcium ionophore and VEGF. A number of studies have shown that phosphorylation of Akt, which is activated by the PI-3K pathway, is required for VEGF-stimulated eNOS activation and NO production [37,51,52].
Since eNOS activity can be regulated by Ca/calmodulin interaction [40,53] and eNOS activation by VEGF requires intracellular calcium mobilization , we determined the effects of TP508 on intracellularcalcium concentration in HCAECs. We found that TP508 treatment did not increase intracellular calcium levels. In contrast, stimulation of cells with VEGF or calcium ionophore caused rapid increases in calcium concentrations. Parallel experiments using the same cells confirmed that VEGF, TP508 and calcium ionophore all increased NO release. These results demonstrate that TP508-induced NO production does not require intracellular calcium mobilization. Calcium-independent stimulation of NO production has previously been reported in endothelial cells stimulated by insulin, estrogen and shear stress [41,42,43,44]. Such a calcium-insensitive eNOS activation has recently been linked to Src phosphorylation of caveolin-1, phosphorylation of Akt and eNOS and to caveolae internalization [45,46]. Since our inhibitor studies showed that TP508-induced activation of NO production was decreased by Src and Akt inhibitors, it may be possible that TP508 activates eNOS through this Src-mediated calcium-insensitive pathway.
Evaluation of TP508 effects on Src and Akt activation showed that TP508 stimulated early transient phosphorylation of both Src and Akt. TP508 has also been shown to stimulate phosphorylation of Akt in adipose tissue-derived stem cells . Moreover, TP508 stimulation of proliferation in these stem cells was inhibited by the same PI-3K inhibitor (LY294002) that inhibited TP508-stimulated NO production in our present studies. As described above, Src and Akt phosphorylations appear to be involved in a number of different pathways that lead to activation of eNOS.
These results suggest that activation of eNOS by TP508 and VEGF both involve the activation of PI-3K/Akt and Src, but TP508 and VEGF activation pathways are distinct with respect to involvement of PKC and calcium mobilization. Studies are underway to more fully elucidate the signaling pathways involved in TP508-stimulated NO production.
The TP508 peptide was originally identified as a fibroblast-receptor-binding domain peptide . This nonproteolytic peptide, however, activated cellular responses that were distinct from those stimulated by thrombin or the protease-activated receptor-1 (PAR1)-activating peptide SFLLRN . Moreover, downregulation of PAR1 inhibited cytokine production in monocytes stimulated by thrombin or SFLLRN, but not cytokine production stimulated by TP508 . TP508, therefore, appears to stimulate its cellular signals by mechanisms that do not involve PAR1 activation. These results led to the hypothesis that nonproteolytic peptides produced at the site of injury by thrombin degradation stimulate non-PARs to activate tissue repair . This hypothesis has been supported by recent photoaffinity cross-linking studies with radiolabeled TP508 which show specific binding of TP508 to a molecule not corresponding in size to that of PARs [Bergmann and Carney, unpubl.].
Although we did not directly compare thrombin and TP508 stimulation of NO production in the present study, our results support the concept that TP508 and thrombin activate different signaling pathways. Previous studies have shown that thrombin stimulates NO production through a mechanism that is independent of PI-3K/Akt activation but dependent on calcium mobilization and activation of PKC-δ [55,56]. Our results showed that TP508 stimulation of early NO production was calcium-independent. Moreover, inhibitor studies suggest that TP508 stimulation may be dependent on activation of PI-3K and PKC-α/β. Thus, activation of eNOS by TP508 appears to differ from thrombin activation in respect to requirement for calcium, activation of PI-3K/Akt and possibly different subtypes of PKC. Additional studies are underway to better define early signaling events leading to stimulation of NO production by TP508 and to determine how cell signaling by TP508 differs from that of thrombin and PAR-activating peptides.
It seems likely that many of the wound-healing effects of TP508, including its effect on diabetic foot ulcers [14,15], may be related to TP508 stimulation of NO production. Results from the initial double-blind placebo-controlled phase 1/2 clinical trial using TP508 for treatment of diabetic foot ulcers showed that twice weekly topical application of TP508 to diabetic foot ulcers resulted in 70% complete closure by 20 weeks compared to a 32% closure in those treated with placebo . Moreover, large ulcers on the heel of feet showed 87% closure (6 of 7) with TP508 treatment and 0% closure (0 of 6) with placebo treatment . This dramatic healing effect suggests that TP508 may stimulate a number of different cellular effects to convert the chronic nonhealing environment to one that actively promotes healing . NO produced locally by topical treatment of TP508 may directly promote chronic ulcer healing since it is required for angiogenesis [22,23,24], recruitment of progenitor cells [57,58] and regulation of metalloproteinase expression [59,60]. Indeed, decreased NO production appears to be a major impediment to chronic wound healing . Moreover, treatment of diabetic wounds with L-arginine to increase NO production enhanced healing both in animal models  and in diabetic foot ulcers .
Although NO-releasing hydrogels alone have been shown to accelerate tissue repair , high levels of NO such as those that are produced by induction of inducible NOS (iNOS) have been shown to inhibit endothelial cell proliferation , tube formation  and wound healing [65,66]. Our studies showed that the levels of NO produced by TP508 stimulation at early time points and after 24 h were comparable to those seen with VEGF treatment. Thus, the stimulation of NO by TP508 is within a range that would be expected to stimulate angiogenesis.
The rapid stimulation of NO production by TP508 in endothelial cells observed in this study may be especially relevant as a mechanism to explain the recently demonstrated myocardial protective effects of TP508 in ischemia-reperfusion injury in normal and hypercholesterolemic pigs [34,35]. In these studies, TP508 perfusion decreased infarct size, improved NO-dependent microvascular function and decreased apoptosis. Previous studies have shown that NO produced by endothelial cells in response to insulin has similar cardioprotective effects . Thus, TP508-stimulated endothelial cell NO production may explain the effects of this peptide on both tissue repair and myocardial protection.
In conclusion, our results demonstrate that TP508 stimulates rapid NO production in a dose-dependent manner in human and bovine endothelial cells. This effect of TP508 is comparable to the effect of VEGF, but unlike VEGF, early TP508 stimulation of NO does not require intracellular calcium mobilization. These studies provide a molecular mechanism by which nonproteolytic thrombin peptides such as TP508 may orchestrate a number of different cellular events leading to tissue revascularization, acceleration of wound healing and myocardial protection.
The authors would like to acknowledge research funding from UTMB Endowment Funds to D.H.C. OrthoLogic Corp., now operating as Capstone Therapeutics of Tempe AZ (NASAQ; CAPS), owns worldwide rights to thrombin peptide TP508 (Chrysalin®, rusalatide acetate). D.H.C. discloses ownership of stock in OrthoLogic. Potential conflicts of interest are managed by The University of Texas Medical Branch Conflict of Interests Committee.
Prof. Darrell H. Carney
Department of Biochemistry and Molecular Biology
The University of Texas Medical Branch
301 University Blvd., Galveston, TX 77555-0645 (USA)
Tel. +1 409 772 3210, Fax +1 409 772 2880, E-Mail email@example.com
Received: March 4, 2009
Accepted after revision: June 2, 2009
Published online: November 6, 2009
Number of Print Pages : 11
Number of Figures : 6, Number of Tables : 0, Number of References : 66
Journal of Vascular Research (Incorporating 'International Journal of Microcirculation')
Vol. 47, No. 3, Year 2010 (Cover Date: April 2010)
Journal Editor: Pohl U. (Munich), Meininger G.A. (Columbia, Mo.)
ISSN: 1018-1172 (Print), eISSN: 1423-0135 (Online)
For additional information: http://www.karger.com/JVR