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Vol. 37, No. 5, 2000
Issue release date: September–October 2000
J Vasc Res 2000;37:372–380

Gene Transfer to Intact Mesenteric Arteries by Electroporation

Martin J.B. · Young J.L. · Benoit J.N. · Dean D.A.
Departments of aPhysiology and bMicrobiology and Immunology, University of South Alabama College of Medicine, Mobile, Ala., USA

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The purpose of the present study was to develop a rapid, reproducible method of nonviral gene transfer to the intact vasculature. Male Sprague-Dawley rats were anesthetized, a midline abdominal incision was made and segmental branches of the superior mesenteric artery were dissected free of surrounding mesentery. A specially designed electroporation probe was placed around the neurovascular bundle and the electroporation chamber filled with a solution containing the firefly luciferase expressing plasmid (pCMV-Lux-DTS) or the green fluorescent protein expressing plasmid (pEGFP-N1). Vessels were electroporated with eight 10-ms pulses of 200 V/cm. Sixty seconds after electroporation, the DNA solution was removed, the intestine returned to the abdomen and the abdominal wall closed with suture and metal wound clips. Six hours to 5 days later, rats were sacrificed and electroporated vessels were recovered. Luciferase activity of the blood vessels was monitored. Gene expression was detected as early as 6 h postelectroporation, peaked at 1–3 days with levels up to 1 ng of reporter gene product per vessel segment and returned towards baseline by day 5. Histological analysis of blood vessel segments revealed green fluorescent protein-positive cells throughout the thickness of the vessel wall (endothelial cells to adventitia). Responses of electroporated vessels to vasoconstricting stimuli were indistinguishable from those of control vessels at either 2 or 40 days posttreatment. The results of this study provide evidence that electroporation is an effective means for introducing naked DNA into the blood vessel wall and form the basis for future studies on targeted gene therapy to the intact vasculature.

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  1. Parker SE, Vahlsing HL, Serfilippi LM, Franklin CL, Doh SG, Gromkowski SH, Lew D, Manthorpe M, Norman J: Cancer gene therapy using plasmid DNA: Safety evaluation in rodents and non-human primates. Hum Gene Ther 1995;6:575–590.
  2. Mir LM, Banoun H, Paoletti C: Introduction of definite amounts of nonpermeant molecules into living cells after electropermeabilization: Direct access to the cytosol. Exp Cell Res 1988;175:15–25.

    External Resources

  3. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K: Current Protocols in Molecular Biology. New York, Wiley, 1994.
  4. Mir LM, Bureau MF, Gehl J, Rangara R, Rouy D, Caillaud J-M, Delaere P, Branellec D, Schwartz B, Scherman D: High-efficiency gene transfer into skeletal muscle mediated by electric pulses. Proc Natl Acad Sci USA 1999;96:4262–4267.
  5. Rizzuto G, Cappelletti M, Maione D, Savino R, Lazzaro D, Costa P, Mathiesen I, Cortese R, Ciliberto G, Laufer R, La Monica N, Fattori E: Efficient and regulated erythropoietin production by naked DNA injection and muscle electroporation. Proc Natl Acad Sci USA 1999;96:6417–6422.
  6. Mathiesen I: Electropermeabilization of skeletal muscle enhances gene transfer in vivo. Gene Ther 1999;6:508–514.

    External Resources

  7. Aihara H, Miyazaki J: Gene transfer into muscle by electroporation in vivo. Nat Biotechnol 1998;16:867–870.
  8. Heller R, Jaroszeski M, Atkin A, Moradpour D, Gilbert R, Wands J, Nicolau C: In vivo gene electroinjection and expression in rat liver. FEBS Lett 1996;389:225–228.
  9. Suzuki T, Shin BC, Fujikura K, Matsuzaki T, Takata K: Direct gene transfer into rat liver cells by in vivo electroporation. FEBS Lett 1998;425:436–440.
  10. Oshima Y, Sakamoto T, Yamanaka I, Nishi T, Ishibashi T, Inomata H: Targeted gene transfer to corneal endothelium in vivo by electric pulse. Gene Ther 1998;5:1347–1354.
  11. Nishi T, Yoshizato K, Yamashiro S, Takeshima H, Sato K, Hamada K, Kitamura I, Yoshimura T, Saya H, Kuratsu J, Ushio Y: High-efficiency in vivo gene transfer using intraarterial plasmid DNA injection following in vivo electroporation. Cancer Res 1996;56:1050–1055.
  12. Harrison RL, Byrne BJ, Tung L: Electroporation-mediated gene transfer in cardiac tissue. FEBS Lett 1998;435:1–5.
  13. Muramatsu T, Nakamura A, Park H: In vivo electroporation: A powerful and convenient means of nonviral gene transfer to tissues of living animals. Int J Mol Med 1998;1:55–62.
  14. Dev NB, Preminger TJ, Hofmann GA, Dev SB: Sustained local delivery of heparin to the rabbit arterial wall with an electroporation catheter. Cathet Cardiovasc Diagn 1998;45:337–345.
  15. Liu MW, Roubin GS, King SB 3rd: Restenosis after coronary angioplasty: Potential biologic determinants and role of intimal hyperplasia. Circulation 1989;79:1374–1387.
  16. Vacik J, Dean BS, Zimmer WE, Dean DA: Cell-specific nuclear import of plasmid DNA. Gene Ther 1999;6:1006–1014.

    External Resources

  17. Manthorpe M, Hartikka J, Vahlsing HL, Sawdey M: Quantification of plasmid DNA expression in vivo; in Ferre F (ed): Gene Quantification. Burhauser, Cambridge, 1996.
  18. Mesh CL, Joh T, Korthuis RJ, Granger DN, Benoit JN: Intestinal vascular sensitivity to vasopressin in portal hypertensive rats. Gastroenterology 1991;100:916–921.
  19. Benoit JN, Womack WA, Korthuis RJ, Wilborn WH, Granger DN: Chronic portal hypertension: Effects on gastrointestinal blood flow distribution. Am J Physiol 1986;250:G535–G539.

    External Resources

  20. Schachtner SK, Rome JJ, Hoyt RF, Newman KD, Virmani R, Dichek DA: In vivo adenovirus-mediated gene transfer via the pulmonary artery of rats. Circ Res 1995;76:701–709.
  21. Lee SW, Trapnell BC, Rade JJ, Virmani R, Dichek DA: In vivo adenoviral vector mediated gene transfer into balloon-injured rat carotid arteries. Circ Res 1993;73:797–807.
  22. Rolling F, Nong Z, Pisvin S, Collen D: Adeno-associated virus-mediated gene transfer into rat carotid arteries. Gene Ther 1997;4:757–761.
  23. Yew NS, Wysokenski DM, Wang KX, Ziegler RJ, Marshall J, McNeilly D, Cherry M, Osburn W, Cheng SH: Optimization of plasmid vectors for high-level expression in lung epithelial cells. Hum Gene Ther 1997;8:575–584.
  24. Daheshia M, Kuklin N, Kanangat S, Manickan E, Rouse BT: Suppression of ongoing ocular inflammatory disease by topical administration of plasmid DNA encoding IL-10. J Immunol 1997;159:1945–1952.
  25. Wolff JA, Ludtke JJ, Acsadi G, Williams P, Jani A: Long-term persistence of plasmid DNA and foreign gene expression in mouse muscle. Hum Mol Genet 1992;1:363–369.

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