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Vol. 74, No. 3, 2009
Issue release date: December 2009
Section title: Paper
Free Access
Brain Behav Evol 2009;74:206–218
(DOI:10.1159/000258667)

Molluscan Memory of Injury: Evolutionary Insights into Chronic Pain and Neurological Disorders

Walters E.T.a · Moroz L.L.b
aDepartment of Integrative Biology and Pharmacology, University of Texas Medical School at Houston, Houston, Tex., and bDepartment of Neuroscience and McKnight Brain Institute, Gainesville, Fla., and Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, Fla., USA
email Corresponding Author

Abstract

Molluscan preparations have yielded seminal discoveries in neuroscience, but the experimental advantages of this group have not, until now, been complemented by adequate molecular or genomic information for comparisons to genetically defined model organisms in other phyla. The recent sequencing of the transcriptome and genome of Aplysia californica, however, will enable extensive comparative studies at the molecular level. Among other benefits, this will bring the power of individually identifiable and manipulable neurons to bear upon questions of cellular function for evolutionarily conserved genes associated with clinically important neural dysfunction. Because of the slower rate of gene evolution in this molluscan lineage, more homologs of genes associated with human disease are present in Aplysia than in leading model organisms from Arthropoda (Drosophila) or Nematoda (Caenorhabditis elegans). Research has hardly begun in molluscs on the cellular functions of gene products that in humans are associated with neurological diseases. On the other hand, much is known about molecular and cellular mechanisms of long-term neuronal plasticity. Persistent nociceptive sensitization of nociceptors in Aplysia displays many functional similarities to alterations in mammalian nociceptors associated with the clinical problem of chronic pain. Moreover, in Aplysia and mammals the same cell signaling pathways trigger persistent enhancement of excitability and synaptic transmission following noxious stimulation, and these highly conserved pathways are also used to induce memory traces in neural circuits of diverse species. This functional and molecular overlap in distantly related lineages and neuronal types supports the proposal that fundamental plasticity mechanisms important for memory, chronic pain, and other lasting alterations evolved from adaptive responses to peripheral injury in the earliest neurons. Molluscan preparations should become increasingly useful for comparative studies across phyla that can provide insight into cellular functions of clinically important genes.

© 2009 S. Karger AG, Basel


  

Key Words

  • Aplysia
  • Evolution
  • Gene loss
  • Genome
  • Injury
  • Nociceptive sensitization
  • Memory
  • Neurological disorders
  • Pain

 Introduction

Mollusca, the second largest animal phylum after Arthropoda, has contributed influential experimental preparations to neuroscience. Large, identifiable neuronal somata and axons in selected molluscs have been exploited as ‘model systems’ to reveal basic mechanisms of neuronal function and plasticity, with some of these discoveries becoming foundations of neurophysiology [Gilbert et al., 1990; Kandel, 2001; Chase, 2002]. Until recently, however, little could be said about how the neural and behavioral phenomena described in molluscs were related evolutionarily to formally similar phenomena in humans. A major limitation was a paucity of the molecular and genomic information needed to identify conserved genes and lineage-specific innovations in molluscs. Moreover, although much research on molluscan nervous systems has been supported by agencies devoted to human health, few studies have explicitly investigated molluscan counterparts of clinically important processes. Nonetheless, some of the basic neuronal mechanisms and associated genes being described in molluscs appear to have functional and/or molecular equivalents that are of considerable clinical significance in humans. Here we consider the use of molluscs to explore conserved and convergent mechanisms that might contribute to selected clinical problems, with particular emphasis on the problem of chronic pain and the possible role of evolutionarily conserved mechanisms shared with memory.

 Molluscan Neurogenomics

The divergence among major bilaterian lineages probably occurred in the Upper Precambrian period (at least 550 million years ago) [Valentine, 2004]. Comparisons of molluscs to other lineages have been slowed, however, by a lack of genetically tractable models and a dearth of genomic information about this phylum. Genome-wide information from at least 2–3 molluscan species is needed to establish evolutionary relationships between molluscan and mammalian genes, molecular pathways, and their functions. Recently the first genomic information from a mollusc became available, with the publication of the neuronal transcriptome and mitochondrial genome of the opisthobranch gastropod, Aplysia californica [Knudsen et al., 2006; Moroz et al., 2006]. This enabled genome-scale profiling of individual, functionally characterized neurons and isolated neuronal processes, opening the way for analyses of the genomic bases of neuronal identity and plasticity in uniquely identified cells. In parallel, the Aplysia genome project was initiated [Moroz et al., 2004], and by 2008 the first stage of sequencing of this genome was completed (http://www.ncbi.nlm.nih.gov/nuccore/AASC00000000.2). In addition, the compact genome of a limpet, Lottia gigantea (in the basal gastropod lineage), has also been sequenced (unpublished, see http://genome.jgi-psf.org/Lotgi1/Lotgi1.info.html), providing an important comparative reference. These advances complement well-known neurophysiological advantages of Aplysia with the ability to specify large gene groups involved in phenomena of interest and to discern patterns of evolution involving these gene products both in molluscs and across phyla.

 Aplysia Neurogenomics, Evolution, and Genes Related to Neurological Diseases

Prominent among the experimental advantages that have established Aplysia as a leading model system for analyzing cellular bases of behavior and plasticity [Kandel, 1976, 2001] are its relatively simple CNS of nine central ganglia containing only about 10,000 neurons [Cash and Carew, 1989], many of which are readily identifiable by soma size and location (fig. 1), electrophysiological properties (fig. 2), synaptic connections (fig. 2) and behavioral effects. More than 200 neurons and hundreds of monosynaptic connections have been individually identified in feeding, locomotor, respiratory, and defensive systems. Compared to Drosophila melanogaster and Caenorhabditis elegans, the two dominant models in molecular neurobiology, three features distinguish Aplysia as a model system: its large neurons, the relatively slow rate of evolution of genes in this lineage (with preservation of an ancestral subset of gene homologs linked to human diseases), and its use of DNA methylation for transcriptional regulation.

The giant sizes of opisthobranch and pulmonate neuronal somata (200–600 μm somata are common) and growth cones (sometimes >600 μm) [Lovell et al., 2006; Lovell and Moroz, 2006] contrast dramatically with those of C. elegans and Drosophila, in which most somata are only 2–5 μm in diameter. Large, individually identifiable neurons facilitate combined physiological, microchemical and genomic measurements [Kandel, 2001; Moccia et al., 2003; Drake et al., 2005; Jezzini et al., 2005; Moroz et al., 2005, 2006; Lovell et al., 2006]. Although giant neurons are also found in other groups (e.g., leeches and crustaceans), the cell bodies of gastropod neurons are fully excitable and, despite their large size, these neurons are electrotonically compact [Adams et al., 1980], permitting unequalled intracellular recordings of synaptic and action potentials from the somata (sometimes simultaneously from multiple neurons in vivo or in vitro for hours at a time). Moreover, the large neurons tolerate repeated microelectrode impalements over several days. These features help drive advances not only in linking neuronal properties to behavior, but in understanding the general cell biology of neurons. For example, neurons from Aplysia and other molluscs have provided insights into axonal regeneration [e.g., Gitler and Spira, 1998; Erez and Spira, 2008], and the cellular functions of human molecules related to disorders such as Alzheimer’s disease, which are being illuminated by testing the effects of injections (e.g., of amyloid precursor protein, beta-amyloid peptide, tau) into giant gastropod somata and cephalopod axons [Satpute-Krishnan et al., 2003; Samarova et al., 2005; Shemesh et al., 2008].

The number of gene homologs shared by mammals with molluscs is greater than the number shared by mammals with Drosophila or C. elegans. Phylogenetic analyses (fig. 1A) position Aplysia as a sister group to the arthropod/nematode clade. This figure illustrates that the evolutionary distance (represented by branch length) from Aplysia to mammals is shorter than the distance from either Drosophila or C. elegans to mammals. This indicates that the amino acid replacement rate has been lower in the lineage leading to Aplysia than in the lineages leading to Drosophila and C. elegans. Additional comparative analysis of evolutionarily conserved genes supports this hypothesis [Moroz et al., 2006]. Greater similarity was also found between mammalian and Aplysia proteins, confirming that the amino acid replacement rate has been lower in the molluscan lineage than in the lineages leading to Drosophila and C. elegans.

FIG01
Fig. 1. Features of a molluscan model organism, Aplysia californica. A Phylogenetic relationships of gastropods (Aplysia) to other prominent model organisms: insects (Drosophila), nematodes (Caenorhabditis elegans), sea urchins (Strongylocentrotus), ascidians (Ciona), vertebrates (represented by dog, Canis, and zebrafish, Danio), and cnidarians (hydrozoan Hydra). The evolutionary distance (indicated as relative branch length) from Canis or Danio to Aplysia is shorter than the distance from the vertebrates to Drosophila or C. elegans, suggesting that the amino acid replacement rate has been lower in the lineage leading to the gastropod Aplysia than in the lineages leading to Drosophila and C. elegans.B Nociceptive sensory neurons in the right ventrocaudal (VC) cluster revealed by in situ hybridization staining for a sensory neuron-specific neuropeptide, sensorin. A somatotopic map (‘Aplunculus’) of the ipsilateral body surface is represented in the cluster. Note that sensorin mRNA is found in neurites and axons throughout the pedal ganglion. C Expression of huntingtin occurs in the giant mucus release motor neuron, LPl1, and other neurons in the left pleural ganglion (in situ hybridization). Scale: 450 μm. D In situ hybridization shows choline acetyltransferase mRNA (a marker for cholinergic neurons) in diverse neurons, including LPl1, in the left pleural and pedal ganglia. Panels A, C and D are modified from Moroz et al. [2006]; panel B is modified from Walters et al. [2004].

The initial analysis of Aplysia gene families revealed unexpected examples of gene loss in Drosophila and C. elegans from a common ancestor of bilaterian animals. Indeed, following the phylogeny outlined in figure 1A, the presence of a gene ortholog shared between Aplysia and mammals, combined with its absence from the genomes of Drosophila or C. elegans, indicate a loss of the orthologs in these lineages rather than its improbable independent origins in molluscs and deuterostomes. Consequently, less derived characteristics of the Aplysia genome might explain a greater conservation of genes related to development (e.g., the transcription factor Churchill), immunity, and human disease in Aplysia [Moroz et al., 2006; Panchin and Moroz, 2008] compared to Drosophila and C. elegans. This suggests that efforts to discern molecular and cellular functions of numerous genes related to human disease could eventually make greater use of Aplysia than Drosophila or C. elegans as model systems.

Table 1 lists some of the human disease-related genes found in the Aplysia transcriptome. Using an annotated subset of neuronal transcripts, numerous orthologs of genes implicated in more than a hundred neurological diseases were identified, including genes relevant to Parkinson’s disease (e.g., Parkin) and Alzheimer’s disease (e.g., presenilins and amyloid precursor protein), as well as fragile X mental retardation protein, huntingtin, the aging-related hormone Klotho, cytokines, and inflammation-related transcripts [Moroz et al., 2006]. Although research on the functions of such genes in molluscs has just begun [e.g., Lee et al., 2003], expression patterns of these genes in identified neurons will suggest avenues for further study. For example, huntingtin is expressed in specific motor neurons, where it is co-localized with FMRFamide and ACh (fig. 1C, D). Also biomedically interesting is a gene for P2X receptors, ATP-gated channels that activate nociceptors to elicit pain in mammals [Burnstock, 2008]. These are expressed in particular neurons in Aplysia, but the functions of these neurons and receptors have yet to be investigated.

TAB01
Table 1. Selected examples of Aplysia homologs of human genes relevant to neurological diseases, with comparisons to other genomic models in Metazoa. The genes marked by asterisks (*) illustrate predicted cases of gene loss in one (*) or more (**) lineages of bilaterian animals. The numbers below each organism name indicate the E value1 for comparison of predicted homologous proteins using the standard BLAST search

A promising advantage of Aplysia as a model organism is the presence of DNA methylation [Moroz et al., 2006; Moroz and Kohn, unpubl. observ.]. Cytosine methylation is thought to play a key role in epigenetic regulatory processes including the silencing of transposons, X-chromosome inactivation, and imprinting. The DNA methyltransferase DNMT1, a crucial protein in this process, is responsible for the maintenance of CpG methylation and has been found in flowering plants, deuterostomes, and a subset of protostomes [Goll and Bestor, 2005]. Surprisingly, DNMT1 and DNA methyltransferases in general are lacking in C. elegans, Schistosoma [Rae and Steele, 1979; Fantappie et al., 2001], and Diptera [Goll and Bestor, 2005; Goll et al., 2006], all of which have only trace levels, if any, of 5-methyl cytosine at CpG sites [Rae and Steele, 1979; Simpson et al., 1986; Suzuki and Bird, 2008]. Homologs for DNMT1, DMAP1 (DNA methyltransferase associating protein), and the transcriptional repressor MBD2 (methyl-CpG-binding domain protein 2) were found in the Aplysia EST database and genome, demonstrating fundamental components of the CpG methylation machinery in Aplysia. Recent data indicate that chromatin remodeling and DNA methylation are involved in memory [Levenson et al., 2006] and possibly in chronic pain [Hosea Blewett, 2008].

These three features are not exclusive to Aplysia; large neurons, neurological disease-related genes, and cellular components for DNA methylation are found in other gastropods, including Tritonia, Pleurobranchaea, Clione, and Lymnaea, and the cephalopod molluscs, Octopus and Nautilus [Moroz and Kohn, unpubl. observ.]. Integration of genomics and physiological studies in individual, functionally characterized neurons in molluscs offers a powerful comparative approach to address molecular and cellular aspects of selected neurological problems.

Clearly, disorders at the network and systems levels contributing to complex neurological diseases are unlikely to be informed by network and systems properties of molluscan nervous systems, which display striking differences from those of other phyla at higher levels of organization. On the other hand, human disorders emanating from cellular and molecular properties of individual neurons that have the same functions in molluscs and mammals can be illuminated by comparisons across these phyla. In the following sections, we discuss similarities between long-term, injury-related plasticity of molluscan and mammalian nociceptors. These similarities point to evolutionarily conserved mechanisms important for both chronic pain and memory.

 Long-Term Nociceptive Sensitization in Molluscs: Functional Similarities to Chronic Pain in Mammals

Most animals exhibit defensive behavioral responses to noxious (damaging or potentially damaging) stimuli. Despite the diversity of body plans and behaviors across Metazoa, ensembles of nociceptive responses often show common patterns [Walters, 1994]. These include rapid local withdrawal followed by escape responses that are accompanied by inhibition of behaviors, such as feeding, that can interfere with escape. Typically, these active defensive behaviors are followed by prolonged immobility and enhanced vigilance, as indicated by sensitization of subsequent defensive responses. This nociceptive sensitization magnifies subsequent responses to innocuous and noxious stimuli, and lowers response thresholds so that innocuous stimuli come to elicit responses that normally would only be evoked by noxious stimuli. Magnified defensive responses (withdrawal and escape locomotion) during nociceptive sensitization have been examined extensively in gastropod molluscs [e.g., Carew et al., 1971; Balaban, 1980; Walters, 1987b]. Moreover, the appearance of new defensive responses to innocuous stimulation during nociceptive sensitization has also been observed in Aplysia [Erickson and Walters, 1988]. These sensitizing effects in molluscs [Walters, 1987a, 1994] are formally similar to the alterations of defensive responses in mammals that are used experimentally as indices for pain hypersensitivity following prior noxious stimulation; i.e., hyperalgesia, defined as an enhancement of normal pain responses to noxious stimuli, and allodynia, defined as the elicitation of pain by normally innocuous stimuli.

Pain is defined as an unpleasant sensory and emotional experience associated with actual or potential tissue damage [Merskey and Bogduk, 1994]. Behavioral indices of pain are used because the subjective component cannot be measured directly. The sensory (nociceptive) component is also a necessary part of the human pain experience under most conditions, and these mechanisms can be determined experimentally and compared across phyla [Walters, 2008]. Investigation of nociceptive mechanisms has begun in a few invertebrates, including the leech [Nicholls and Baylor, 1968; Pastor et al., 1996], Drosophila [Tracey et al., 2003; Babcock et al., 2009], C. elegans [reviewed by Tobin and Bargmann, 2004] and, as described below, Aplysia.

Human pain caused by injury usually persists no longer than the period of healing. Such pain is generally considered to be adaptive, as suggested by the high morbidity and short lives of the rare individuals born without functioning nociceptors [Woolf and Ma, 2007]. In contrast to acute pain, chronic pain can persist long after apparent healing, bringing no obvious benefit to sufferers. Chronic pain is quite common, occurring in ∼20% of the world’s population [Breivik et al., 2006], and it remains highly resistant to treatment. Mechanisms underlying the persistence of chronic pain are unknown; however, some forms of chronic pain are associated with persistent hyperexcitability of nociceptors, which might play a large role in driving such pain [Ali et al., 1999; Sung and Ambron, 2004; Ochoa et al., 2005; Shim et al., 2005; Walters, 2008; Walters et al., 2008]. As reviewed below, in Aplysia long-term hyperexcitability (LTH) of nociceptors has been associated with a form of memory, long-term sensitization of defensive behavior.

Mechanosensory neurons comprising the left E (LE) cluster in the abdominal ganglion [Byrne et al., 1974] and the ventrocaudal (VC) cluster in each pleural ganglion [Walters et al., 1983] have been investigated intensively in studies of learning and memory mechanisms [see Kandel, 2001]. Somata in the large, apparently homogeneous pleural VC clusters conveniently form a somatotopic map of the ipsilateral body surface, an ‘aplunculus’ (fig. 1B) [Walters et al., 2004]. Although the VC neurons and LE neurons (which innervate the siphon) are often regarded as receptors for light touch by investigators of learning and memory [e.g., Antonov et al., 2001], there are two reasons why these primary sensory neurons are properly considered to be nociceptors. First, except when sensitized, they exhibit relatively high thresholds, graded responses to increasing stimulus intensities, and maximal responses to pinching stimuli that cause clear tissue damage. Second, these cells share a property that among mechanosensory neurons is unique to nociceptors – sensitization to repeated stimulation (fig. 2B); other mechanosensory neurons adapt without sensitizing when repeatedly activated [Illich and Walters, 1997]. This sensitization by prior noxious stimulation, along with maximal activation by noxious stimuli, are characteristic features of nociceptors [Woolf and Ma, 2007].

FIG02
Fig. 2. Sensorin-expressing neurons in Aplysia are ‘true’ nociceptors and exhibit long-term nociceptive sensitization. A Body regions innervated by different sensory clusters. Sensory neurons innervating the siphon (LE cluster) and tail (VC cluster) have received the most study. B Evidence that mechanosensory neurons are nociceptors. Responses of an LE siphon sensory neuron (SN) to mechanical stimulation (B1) exhibit a relatively high threshold, are maximal with damaging stimuli (pinch), and increase (sensitize) following the pinch (B1 and B2) [modified from Illich and Walters, 1997]. C Long-term, sensitizing effects in a nociceptive tail sensory neuron. One day after noxious tail shock a SN innervating the shocked site, but not a contralateral control SN, exhibited afterdischarge following a brief (10-msec) test pulse to the soma, and pronounced facilitation of its synapse onto a tail motor neuron (MN) [modified from Walters, 1987b]. D Intrinsic injury signals are sufficient to produce SN hyperexcitability. Example showing how the spike accommodation normally seen in control SNs is greatly reduced in a dissociated SN one day after transection of its neurites [modified from Ambron et al., 1996].

Another similarity between rat and Aplysia nociceptors is their shared capacity to store long-term cellular ‘memory’ of noxious stimulation. Persistent inflammatory signals impinging on peripheral branches of nociceptors, rather than long-term alterations intrinsic to the nociceptors, are often assumed to drive persistent pain in mammals [e.g., Marchand et al., 2005]. Peripheral injury and inflammation alter gene expression within nociceptors, however, upregulating some ion channels and growth factor receptors [Waxman et al., 1994; Mannion et al., 1999; Woolf and Costigan, 1999; Ji et al., 2002]. Moreover, peripheral nerve injury and inflammation produce regenerative and collateral growth of nociceptor axons [Shea and Perl, 1985; Doucette and Diamond, 1987; Lu and Richardson, 1991] and a transcription-dependent enhancement of the nociceptor’s growth state that continues to be expressed in vitro after isolation of the neurons [Smith and Skene, 1997]. In addition, LTH of dissociated sensory neurons following prior injury or inflammation has been documented [Ma and LaMotte, 2005; Walters et al., 2008]. Although such observations provide strong evidence for intrinsic cellular ‘memory’, they do not exclude the possibility that the memory lasts only long enough to amplify LTH initiated by the trauma of dissociation [Zheng et al., 2007].

Evidence that Aplysia sensory neurons display long-term alterations such as those associated with hyperalgesia in mammalian nociceptors came with the finding that nociceptors activated intensely by tail shock display synaptic facilitation and hyperexcitability of their cell soma lasting at least a day (fig. 2C) [Walters, 1987a]. Peripheral injury, produced by either pinching and cutting the tail or by crushing the nerve that innervates the tail, produces effects on the nociceptors that last weeks or longer. These include axonal regeneration [Steffensen et al., 1995] and sprouting of neurites near a site of peripheral injury and within central ganglia [Billy and Walters, 1989; Steffensen et al., 1995]. Peripheral injury also causes a decrease in mechanosensory threshold in the damaged region [Billy and Walters, 1989; Dulin et al., 1995], a decrease in electrical threshold of the nociceptor axon near a site of injury or of intense, transient depolarization [Weragoda et al., 2004], and an increase in excitability of the nociceptor soma [Walters et al., 1991]. Soma hyperexcitability promotes afterdischarge (fig. 2C) to afferent action potentials, amplifying nociceptive input to the CNS [Gasull et al., 2005]. Finally, peripheral injury can produce synaptic facilitation (fig. 2C) [Walters et al., 1991]. The existence of intrinsic mechanisms for hyperexcitability in Aplysia nociceptors is demonstrated by the induction of LTH in isolated, dissociated neurons by transection of their neurites (fig. 2D) [Ambron et al., 1996; Bedi et al., 1998] or transient depolarization [Kunjilwar et al., 2009]. Additional evidence comes from the expression of LTH after peripheral injury or transient depolarization in ganglia-nerve preparations under low-Ca2+ conditions likely to block continuing release of neuromodulators [Weragoda et al., 2004; Gasull et al., 2005; Kunjilwar et al., 2009].

Functional similarities of long-term plasticity in Aplysia and rat nociceptors after injury suggested that severe noxious stimulation might switch diverse types of nociceptors into a persistent, intrinsically maintained hyperfunctional state. This idea is being tested in a rat model of chronic pain induced by spinal cord injury. Chronic neuropathic pain after central damage, which occurs in a majority of human patients after spinal cord injury, was not previously thought to involve changes in nociceptors. A novel prediction was that prolonged exposure of the central axons and terminals of nociceptors to signs of tissue injury (e.g., inflammatory signals) would lead to LTH that could result in persistent spontaneous activity being generated in nociceptor somata (and enhanced growth of their processes), even when isolated from extrinsic inputs. Preliminary support for this prediction [Walters et al., 2008] raises the possibility that nociceptors might be a useful target for treating this often intractable form of chronic pain.

 Did Fundamental Mechanisms Underlying Memory and Chronic Pain Evolve from Ancestral Responses to Peripheral Injury?

Striking similarities exist in the behavioral and cellular responses of Aplysia, rats, and other animals to noxious stimulation [Walters, 1994, 2008]. Furthermore, these similarities are paralleled by functional similarities in their nociceptors. The similarities are likely to reflect contributions both from convergent, independently derived mechanisms and from ancestral, evolutionarily conserved (homologous), molecular mechanisms. Here we review evidence for shared signaling pathways that induce nociceptive plasticity, point out that these apparently homologous signals also play roles in triggering long-term memory in the CNS, and discuss peripheral injury as a ubiquitous selection pressure that might have shaped ancestral plasticity mechanisms.

Both in nociceptors and memory circuits, plasticity mechanisms cause short- and long-term neuronal alterations: enhancement of synaptic transmission [e.g., Ji et al., 2003; Lee and Silva, 2009], enhancement of membrane excitability [e.g., Xu and Kang, 2005; Devor, 2006], and growth or regrowth of synapses and processes [Bailey and Kandel, 2008; De Roo et al., 2008]. These alterations are induced by numerous signals that are common to Aplysia sensorimotor systems, mammalian spinal sensory systems, and mammalian circuits in the hippocampus and other parts of the brain important for learning and memory. Some of the general initiating signals that are common to molluscan and mammalian nociceptors, and to neurons displaying long-term plasticity in central neurons of diverse animals are shown in figure 3. Initiating events for long-term alterations include intense depolarization and Ca2+ entry consequent to membrane injury or binding of excitatory amino acids, and activation of cell signaling pathways by binding of neuromodulators and growth factors released by neurons [Jankowsky and Patterson, 1999; Kandel, 2001; Weragoda and Walters, 2007] and other cell types [e.g., glia, support cells, and inflammatory cells; Bradley and Finkbeiner, 2002; Gibbs et al., 2008]. Changes in gene expression are triggered by Ca2+ entry accompanying action potentials propagating into the soma [Saha and Dudek, 2008], as well as retrograde signals transported to the nucleus from sites of axonal injury [Gunstream et al., 1995; Ambron and Walters, 1996; Lin et al., 2003; Sung and Ambron, 2004] or intense synaptic stimulation [Otis et al., 2006; Lai et al., 2008]. Long-term alterations can include effects localized to sites of injury or to intensely activated synapses, as well as global actions affecting much of the neuron from neural or endocrine signals released throughout the body during either injury or the formation of central memories.

FIG03
Fig. 3. Similarity of initial signals for induction of long-term neuronal plasticity in molluscan and mammalian nociceptors (left side) and mammalian neurons that form memories within the CNS (right side). In each case local signals include intense depolarization of an injured segment or synaptic region (darkened areas), Ca2+ influx, and modulation by various neuroactive substances released from nearby cells. In addition, global signals released during injury or memorable events can influence the soma and other parts of the neuron, at least in part by regulating gene transcription. These initiating events for nociceptive sensitization and memory are mediated by common sets of highly conserved intracellular signals (see text).

Intracellular plasticity signals common to injury and memory include Ca2+ entering through ionotropic glutamate receptor-gated channels opened during intense electrical activity [Moroz et al., 1993; Ji et al., 2003; Ha et al., 2006; Rao and Finkbeiner, 2007; Glanzman, 2008], and activation of cell signaling pathways by Ca2+ transients and by the binding of ligands to G-protein-coupled receptors and receptor tyrosine kinases [Ji et al., 2003; Purcell and Carew, 2003; Barco et al., 2006; Pezet and McMahon, 2006; Lu et al., 2008]. The known downstream signals associated with injury and memory are also highly conserved, including activated protein kinase or lipid kinase enzymes, notably PKA, PKC, ERK and PI3K [Obata and Noguchi, 2004; Barco et al., 2006; Cheng and Ji, 2008; Sossin, 2008; Lee and Silva, 2009]. Less extensive evidence suggests that NO, cGMP, and PKG [Aley et al., 1998; Lewin and Walters, 1999; Sung and Ambron, 2004; Sung et al., 2004; Zheng et al., 2007; Antonov et al., 2007; Ota et al., 2008] and a protein kinase, TOR (mTOR in mammals), which promotes local protein synthesis in axons and dendrites [Casadio et al., 1999; Weragoda et al., 2004; Hu et al., 2007; Price et al., 2007; Jimenez-Diaz et al., 2008; Sossin, 2008; Costa-Mattioli et al., 2009], also contribute to both nociceptor sensitization and conventional memory. Long-term effects usually require changes in gene transcription, with the transcription factor CREB playing an important role in prominent forms of long-term plasticity in mammalian brain [Alberini, 2009; Lee and Silva, 2009] and Aplysia nociceptors [Casadio et al., 1999; Lewin and Walters, 1999; Barco et al., 2006], and play a role in nociceptive plasticity of mammalian nociceptors as well [Tamura et al., 2005; Teng and Tang, 2006]. Thus, at the subcellular and molecular levels, induction mechanisms for long-term plasticity appear remarkably similar when comparing molluscs to mammals, and comparing nociceptors to hippocampal neurons.

These molecular similarities support the proposal that fundamental mechanisms important for memory and chronic pain evolved from adaptive responses to injury in the earliest animals [Walters, 1991]. Injury-related selection pressures might be both powerful and ubiquitous; an animal that after injury cannot compensate for loss of sensory function or utilize nociceptive sensitization to reduce chances of further injury is likely to be less successful biologically than one that does. Unlike pressures to store information about changes in the environment or about consequences of behavioral actions, which would have had little impact until neural circuits complex enough to store such information had evolved, injury-related selection pressures have probably operated on neurons (and antecedent cells) from the earliest stages of animal evolution. In other words, plasticity mechanisms selected as adaptive responses to injury could have appeared long before the origin of nervous systems complex enough to support forms of learning and memory involving the integration of diverse sources of information (e.g., associative learning).

It seems likely that the earliest neurons were cells with both sensory and motor functions in small early metazoans that lacked shells or hard exoskeletons. Branches of these neurons would have been exposed directly to peripheral trauma from inanimate, and possibly animate, sources. Thus, plasticity mechanisms might have been selected in early neurons for their ability to (1) repair and regenerate damaged axonal branches, (2) compensate for loss of sensory function within a damaged region, (3) protect against self-inflicted damage (e.g., by suppressing movements of body parts with open wounds), and (4) boost responses to attacks by predators or parasites attracted by a wound [Walters, 1991, 1994; Weragoda et al., 2004]. The first set of mechanisms would result in regrowth of lost axonal branches, and the other sets could include (1) hyperexcitability of an injured neuron’s surviving branches, (2) hyperexcitability of the branches of nearby, undamaged sensory neurons, (3) hyperexcitability of the soma or central branches of sensory neurons (which could amplify trains of sensory action potentials arriving from the periphery), (4) enhanced release of neurotransmitters from central synapses of sensory neurons, and (5) growth of new synapses from surviving sensory neurons.

None of the molecular signals and cellular effectors associated thus far with nociceptor plasticity or with memory is restricted to neuronal plasticity; each has many other roles and is found in most metazoan cells. Known molecular signals identified with neuronal plasticity (second messengers, protein kinases, protein phosphatases, transcription factors) represent parts of highly conserved, core regulatory modules [e.g., Gerhart and Kirschner, 1997], which are also widely involved in development, differentiation, adaptation to different physiological conditions, and cellular responses to stress. In primeval neurons, signaling modules activated by signs of cellular damage or tissue injury (e.g., extracellular exposure to K+, amino acids, and other intracellular constituents released from ruptured cells, depolarization, Ca2+ influx – see fig. 3) might have become linked to cellular effectors that could mount the adaptive neuronal responses described above. An intriguing possibility is that injury-activated plasticity systems, selected early in evolution for peripheral functions, were ‘pre-adapted’ for later use in more complex, central forms of neural plasticity. In the CNS such plasticity would then be activated by normal and pathological release of intercellular signals – such as glutamate and growth factors – that were signs of tissue injury in the first multicellular organisms, and later became adapted for signaling between neurons as nervous systems evolved. Comparative studies across Mollusca and other phyla will be important for testing such evolutionary hypotheses, and should offer insights into the functions and dysfunctions of highly conserved plasticity systems that might be shared with humans.

 Acknowledgements

We are grateful to Drs. Robyn Crook and Paul Katz for helpful comments. E.T.W. is supported by NIH grants 1RO1NS3599 and R21NS061200, and a grant from the Christopher Reeve Foundation. L.L.M. is supported by NIH and NSF grants 1RO1NS06076, R21RR025699, NSF-0744649, and in part by the McKnight Brain Research Foundation and UF Opportunity Funds.


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Author Contacts

Edgar T. Walters, PhD
Department of Integrative Biology and Pharmacology
University of Texas Medical School at Houston, 6431 Fannin St, MSB 4.116
Houston, TX 77030 (USA)
Tel. +1 713 500 6314, Fax +1 713 500 7444, E-Mail edgar.t.walters@uth.tmc.edu

  

Article Information

Published online: December 21, 2009
Number of Print Pages : 13
Number of Figures : 3, Number of Tables : 1, Number of References : 119

  

Publication Details

Brain, Behavior and Evolution

Vol. 74, No. 3, Year 2009 (Cover Date: December 2009)

Journal Editor: Wilczynski W. (Atlanta, Ga.), Striedter G.F. (Irvine, Calif.)
ISSN: 0006-8977 (Print), eISSN: 1421-9743 (Online)

For additional information: http://www.karger.com/BBE


Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

Abstract

Molluscan preparations have yielded seminal discoveries in neuroscience, but the experimental advantages of this group have not, until now, been complemented by adequate molecular or genomic information for comparisons to genetically defined model organisms in other phyla. The recent sequencing of the transcriptome and genome of Aplysia californica, however, will enable extensive comparative studies at the molecular level. Among other benefits, this will bring the power of individually identifiable and manipulable neurons to bear upon questions of cellular function for evolutionarily conserved genes associated with clinically important neural dysfunction. Because of the slower rate of gene evolution in this molluscan lineage, more homologs of genes associated with human disease are present in Aplysia than in leading model organisms from Arthropoda (Drosophila) or Nematoda (Caenorhabditis elegans). Research has hardly begun in molluscs on the cellular functions of gene products that in humans are associated with neurological diseases. On the other hand, much is known about molecular and cellular mechanisms of long-term neuronal plasticity. Persistent nociceptive sensitization of nociceptors in Aplysia displays many functional similarities to alterations in mammalian nociceptors associated with the clinical problem of chronic pain. Moreover, in Aplysia and mammals the same cell signaling pathways trigger persistent enhancement of excitability and synaptic transmission following noxious stimulation, and these highly conserved pathways are also used to induce memory traces in neural circuits of diverse species. This functional and molecular overlap in distantly related lineages and neuronal types supports the proposal that fundamental plasticity mechanisms important for memory, chronic pain, and other lasting alterations evolved from adaptive responses to peripheral injury in the earliest neurons. Molluscan preparations should become increasingly useful for comparative studies across phyla that can provide insight into cellular functions of clinically important genes.

© 2009 S. Karger AG, Basel


  

Author Contacts

Edgar T. Walters, PhD
Department of Integrative Biology and Pharmacology
University of Texas Medical School at Houston, 6431 Fannin St, MSB 4.116
Houston, TX 77030 (USA)
Tel. +1 713 500 6314, Fax +1 713 500 7444, E-Mail edgar.t.walters@uth.tmc.edu

  

Article Information

Published online: December 21, 2009
Number of Print Pages : 13
Number of Figures : 3, Number of Tables : 1, Number of References : 119

  

Publication Details

Brain, Behavior and Evolution

Vol. 74, No. 3, Year 2009 (Cover Date: December 2009)

Journal Editor: Wilczynski W. (Atlanta, Ga.), Striedter G.F. (Irvine, Calif.)
ISSN: 0006-8977 (Print), eISSN: 1421-9743 (Online)

For additional information: http://www.karger.com/BBE


Article / Publication Details

First-Page Preview
Abstract of Paper

Published online: 12/21/2009
Issue release date: December 2009

Number of Print Pages: 13
Number of Figures: 3
Number of Tables: 1

ISSN: 0006-8977 (Print)
eISSN: 1421-9743 (Online)

For additional information: http://www.karger.com/BBE


Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

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