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Vol. 97, No. 4, 2010
Issue release date: June 2010

Fatal Effects of a Neonatal High-Protein Diet in Low-Birth-Weight Piglets Used as a Model of Intrauterine Growth Restriction

Jamin A. · D’Inca R. · Le Floc’h N. · Kuster A. · Orsonneau J.-L. · Darmaun D. · Boudry G. · Le Huërou-Luron I. · Sève B. · Gras-Le Guen C.
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Abstract

Background: Although full-term infants suffering intrauterine growth restriction (IUGR) are routinely fed high-protein (HP) formulas to ensure catch-up growth, the effects of HP intake are poorly understood. An IUGR piglet model provides an opportunity to investigate these effects. Methods and Results: Twelve IUGR piglets were artificially fed HP formulas (50% more protein in comparison to sow milk) from the 2nd day of life (d2) until d28. Unexpectedly, all HP piglets developed poor growth, severe hypotonia and polypnea between d10 and d16. One third died spontaneously. This syndrome was investigated to understand its pathophysiology and to adopt a strategy to restore health. Blood and urine biochemistry and amino acid concentrations were investigated in 10 HP piglets and 8 piglets that were fed a normal-protein (NP) formula. In comparison to NP piglets, HP piglets showed significant hypokalemia (2.7 ± 0.6 vs. 3.6 ± 0.6 mmol/l; p < 0.01), hypophosphatemia (1.5 ± 0.2 vs. 3.0 ± 0.3 mmol/l; p > 0.01), hypercalcemia (3.0 ± 0.3 vs. 2.5 ± 0.2 mmol/l; p < 0.01), hyperammonemia (365 ± 4 vs. 242 ± 15 µmol/l; p < 0.05), elevated blood urea (6.5 ± 0.4 vs. 1.3 ± 0.4 mmol/l; p < 0.01) and elevated taurine concentrations (50.2 ± 8.5 vs. 17.7 ± 2.7 µmol/l; p < 0.01). Conclusions: These altered parameters indicated inadequate potassium and phosphorus dietary supplies in HP piglets. When the HP formula was supplemented with monocalcium phosphate and monopotassium phosphate (HP-sup), serum biochemistry was normalized in piglets fed this formula (n = 8). This experimental strategy restored growth in IUGR piglets fed HP-sup, without a toxic effect. The current findings suggest that use of an HP formula without a proportional increase in its phosphorus and potassium content induces pathology similar to the refeeding syndrome in IUGR piglets.



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References

  1. Albertsson-Wikland K, Wennergren G, Wennergren M, Vilbergsson G, Rosberg S: Longitudinal follow-up of growth in children born small for gestational age. Acta Paediatr 1993;82:438–443.
  2. Thureen P, Heird WC: Protein and energy requirements of the preterm/low birth weight (LBW) infant. Pediatr Res 2005;57:95–98.

    External Resources

  3. Barker DJ, Clark PM: Fetal undernutrition and disease in later life. Rev Reprod 1997;2:105–112.
  4. Wells JC, Chomtho S, Fewtrell MS: Programming of body composition by early growth and nutrition. Proc Nutr Soc 2007;66:423–434.
  5. Bauer R, Walter B, Hoppe A, Gaser E, Lampe V, Kauf E, Zwiener U: Body weight distribution and organ size in newborn swine (Sus scrofa domestica) – a study describing an animal model for asymmetrical intrauterine growth retardation. Exp Toxicol Pathol 1998;50:59–65.
  6. Burke C, Sinclair K, Cowin G, Rose S, Pat B, Gobe G, Colditz P: Intrauterine growth restriction due to uteroplacental vascular insufficiency leads to increased hypoxia-induced cerebral apoptosis in newborn piglets. Brain Res 2006;1098:19–25.
  7. Gondret F, Lefaucheur L, Juin H, Louveau I, Lebret B: Low birth weight is associated with enlarged muscle fiber area and impaired meat tenderness of the longissimus muscle in pigs. J Anim Sci 2006;84:93–103.
  8. Bauer R, Walter B, Brust P, Fuchtner F, Zwiener U: Impact of asymmetric intrauterine growth restriction on organ function in newborn piglets. Eur J Obstet Gynecol Reprod Biol 2003;110:S40–S49.
  9. Poore KR, Fowden AL: The effects of birth weight and postnatal growth patterns on fat depth and plasma leptin concentrations in juvenile and adult pigs. J Physiol 2004;558:295–304.
  10. Noblet J, Etienne M: Estimation of sow milk nutrient output. J Anim Sci 1989;67:3352–3359.
  11. Dourmad JY, Noblet J, Etienne M: Effect of protein and lysine supply on performance, nitrogen balance, and body composition changes of sows during lactation. J Anim Sci 1998;76:542–550.
  12. Blat S, Malbert CH: The vagus is inhibitory of insulin secretion under fasting conditions. Am J Physiol Endocrinol Metab 2001;281:E782–E788.
  13. Le Floc’h N, Obled C, Seve B: In vivo threonine oxidation rate is dependent on threonine dietary supply in growing pigs fed low to adequate levels. J Nutr 1995;125:2550–2562.
  14. Raiha NC, Heinonen K, Rassin DK, Gaull GE: Milk protein quantity and quality in low-birthweight infants. I. Metabolic responses and effects on growth. Pediatrics 1976;57:659–684.
  15. Davis JA, Greer FR, Benevenga NJ: Urea production is increased in neonatal piglets infused with alanine at 25, 50, and 75% of resting energy needs. J Nutr 2000;130:1971–1977.
  16. Davis JA, Greer FR, Benevenga NJ: Comparison of total body urea production potential with total body carbamoyl phosphate synthetase (CPS-1) activity in newborn piglets infused with alanine at 50% of resting energy expenditure for 36 hours. J Nutr 2000;130:1978–1983.
  17. Boehm G, Muller DM, Teichmann B, Krumbiegel P: Influence of intrauterine growth retardation on parameters of liver function in low birth weight infants. Eur J Pediatr 1990;149:396–398.
  18. Moore FD, Edelman IS, Olney JM, James AH, Brooks L, Wilson GM: Body sodium and potassium. III. Interrelated trends in alimentary, renal and cardiovascular disease; lack of correlation between body stores and plasma concentration. Metabolism 1954;3:334–350.
  19. Crook MA, Hally V, Panteli JV: The importance of the refeeding syndrome. Nutrition 2001;17:632–637.
  20. Korbonits M, Blaine D, Elia M, Powell-Tuck J: Metabolic and hormonal changes during the refeeding period of prolonged fasting. Eur J Endocrinol 2007;157:157–166.
  21. Seve B, Reeds PJ, Fuller MF, Cadenhead A, Hay SM: Protein synthesis and retention in some tissues of the young pig as influenced by dietary protein intake after early-weaning. Possible connection to the energy metabolism. Reprod Nutr Dev 1986;26:849–861.
  22. Wu G, Ott TL, Knabe DA, Bazer FW: Amino acid composition of the fetal pig. J Nutr 1999;129:1031–1038.
  23. Oppenheimer SJ, Snodgrass GJ: Neonatal rickets. Histopathology and quantitative bone changes. Arch Dis Child 1980;55:945–949.
  24. Boissinot C, Cheron G, Garabedian M, Steru D, Lenoir G: Hypophosphatemic rickets in premature infants independent of vitamin D (in French). Arch Fr Pediatr 1984;41:557–559.
  25. Odink J, Smeets JF, Visser IJ, Sandman H, Snijders JM: Hematological and clinicochemical profiles of healthy swine and swine with inflammatory processes. J Anim Sci 1990;68:163–170.
  26. Lombardini JB: Taurine levels in blood and urine of vitamin B6-deficient and estrogen-treated rats. Biochem Med Metab Biol 1986;35:125–131.
  27. Yamaguchi K, Shigehisa S, Sakakibara S, Hosokawa Y, Ueda I: Cysteine metabolism in vivo of vitamin B6-deficient rats. Biochim Biophys Acta 1975;381:1–8.
  28. Schaffer S, Takahashi K, Azuma J: Role of osmoregulation in the actions of taurine. Amino Acids 2000;19:527–546.
  29. Chesney RW, Helms RA, Christensen M, Budreau AM, Han X, Sturman JA: The role of taurine in infant nutrition. Adv Exp Med Biol 1998;442:463–476.
  30. Stipanuk MH: Sulfur amino acid metabolism: pathways for production and removal of homocysteine and cysteine. Annu Rev Nutr 2004;24:539–577.
  31. Boehm G, Melichar V, Lorenz I, Muller D, Beyreiss K: Nutrition of newborns small for gestational age with human milk lyophilisate enriched human milk during the first week of life. Acta Paediatr Hung 1985;26:261–269.
  32. Lindblad BS, Hagelberg S, Lundsjo A: Blood levels of critical amino acids in very low birthweight infants on a high human milk protein intake. Acta Paediatr Scand Suppl 1982;296:24–27.
  33. Maggio L, Cota F, Gallini F, Lauriola V, Zecca C, Romagnoli C: Effects of high versus standard early protein intake on growth of extremely low birth weight infants. J Pediatr Gastroenterol Nutr 2007;44:124–129.


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