Background/Aim: When patients with chronic hepatitis C (CHC) are treated with interferon (IFN)-based therapy, achieving serum HCV-RNA negativity by week 12 (early viral response, EVR) is an important predictor of a sustained virologic response. The aim of this study was to clarify whether changes in IFN-α receptor 2 (IFNAR-2) expression by peripheral blood monocytes (Mo) and the EVR rate differed between patients with genotype 1b and a high viral load showing substitution of amino acid 70 in the core region of HCV (mutant, n = 20) and patients without this substitution (wild, n = 23). Patients and Methods: Forty-three CHC patients were studied, and received pegylated IFN plus ribavirin. IFNAR-2 expression by Mo was determined using flow cytometry to measure the mean fluorescence intensity (MFI) before and up to 28 days after starting therapy. Results: The EVR rate of the mutant group was significantly lower than that of the wild group (35 vs.70%). The MFI of Mo was significantly higher in the wild group than in the mutant group before and also 3, 7, and 28 days after starting therapy. Conclusions: Mutation of HCV was related to lower IFNAR-2 expression by Mo before and after starting therapy.
© 2009 S. Karger AG, Basel
- Chronic hepatitis C
- Amino acid substitution
- Core region
- Interferon-α receptor 2
- Peripheral blood leukocyte
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Koji Ishii, MD
Department of Internal Medicine, Division of Gastroenterology and Hepatology
6-11-1 Omorinishi, Otaku
Tokyo 143 (Japan)
Tel. +81 3 3762 4151, Fax +81 3 3763 8542, E-Mail email@example.com
Received: August 5, 2009
Accepted after revision: October 12, 2009
Published online: December 3, 2009
Number of Print Pages : 6
Number of Figures : 3, Number of Tables : 1, Number of References : 21
Intervirology (International Journal of Basic and Medical Virology)
Vol. 53, No. 2, Year 2010 (Cover Date: February 2010)
Journal Editor: Liebert U.G. (Leipzig)
ISSN: 0300-5526 (Print), eISSN: 1423-0100 (Online)
For additional information: http://www.karger.com/INT
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