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Table of Contents
Vol. 17, No. 2, 1985
Issue release date: 1985
Ophthalmic Res 1985;17:90–95

In vivo Assessment of Lens Transmission for Blue-Green Light by Autofluorescence Measurement

van Best J.A. · Tsoi E.W.S.J.T.A. · Boot J.P. · Oosterhuis J.A.
Department of Ophthalmology, Leiden University Medical Centre, Leiden, The Netherlands

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A simple quantitative and reproducible method for evaluating lens transmission (λ = 490 and 530 nm) is described. It is based on the measurement of autofluorescence in the anterior and the posterior part of the lens by means of a fluorophotometer, assuming an about equal fluorescence quantum efficiency in both parts. Consequently any difference in fluorescence between both parts can be attributed to a loss of exciting and fluorescent light in the lens. For both wavelengths, the average lens transmission is determined by this method in a normal population as a function of age.

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