Use of Infrared Attenuated Total Reflectance Spectroscopy for the in vivo Measurement of Hydration Level and Silicone Distribution in the Stratum corneum following Skin Coverage by Polymeric DressingsBranagan M. · Chenery D.H. · Nicholson S.
Smith and Nephew Group Research Centre, York Science Park, Heslington, York, UK
The ability of wound dressings to hydrate the stratum corneum has been tested experimentally using attenuated total reflectance (ATR) infrared spectroscopy. A silicone gel dressing was able to hydrate the stratum corneum to the extent that its water content within the sampled volume was >60% after 5 h of contact compared with about 15% of normal exposed skin and about 30% for a highly permeable hydrophilic polyurethane dressing. An ATR method was devised to determine the presence and depth of penetration of silicone in the stratum corneum as a result of skin coverage by the polymeric dressing.
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