To view the fulltext, please log in
To view the pdf, please log in
Background and Objectives: The mechanism for the destruction of a patient's own platelets in post–transfusion purpura (PTP) is unknown. In order to test the hypothesis that the destruction of autologous platelets in PTP is related to the presence of platelet antibodies with pan–reactivity, we investigated sera from patients with PTP. Materials and Methods: Sera from 12 patients with PTP were investigated for platelet antibodies by platelet–ELISA and monoclonal antibody–specific immobilization of platelet antigen assay. Results: During the thrombocytopenic phase, antibodies of IgG and IgM classes with pan–specificity against platelet GPIIb–IIIa, GPIb–IX and GPIa–IIa were found together with HPA alloantibodies. After recovery, the pan–specific antibodies disappeared or the extent of reactivity diminished, whereas the IgG HPA alloantibodies persisted. Conclusion: These findings provide evidence that transient panreactive antibodies may be responsible for the autologous platelet destruction in PTP.
Copyright / Drug Dosage
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.
- Bierling P, Godeau B, Fromont P, Bettaieb A, Debili N, el Kassar N, Rouby JJ, Vainchenker W, Duedari N: Posttransfusion purpura–like syndrome associated with CD36 (Naka) isoimmunization. Transfusion 1995;35:777–782.
- Taaning E: Microplate enzyme immunoassay for detection of platelet antibodies. Tissue Antigens 1985;25:19–27.
- Kiefel V, Santoso S, Weisheit M, Mueller Eckhardt C: Monoclonal antibody–specific immobilization of platelet antigens (MAIPA): A new tool for the identification of platelet–reactive antibodies. Blood 1987;70:1722–1726.
- Gengozian N, McLaughlin CL: IgG + platelets in the marmoset: Their induction, maintenance and survival. Blood 1980;55:885–890.
- Gengozian N, Annostby D: Antibodies selectively reactive to autologous and host–type platelets are obtained following interspecies immunization in marmosets. Clin Exp Immunol 1981;43:128–134.
- Pegels JG, Bruynes EC, Engelfriet CP, von dem Borne AE: Post–transfusion purpura: A serological and immunochemical study. Br J Haematol 1981;49:521–530.
- Perry RM: The occurrence and significance of platelet and granulocyte specific antibodies in bone marrow transplant patients; PhD thesis. University of London, 1983.
- Berney SI, Metcalfe P, Wathen NC, Waters AH: Posttransfusion purpura responding to high dose intravenous IgG: Further observations on pathogenesis. Br J Haematol 1985;61: 627–632.
- Stricker RB, Lewis BH, Corash L, Shuman MA: Posttransfusion purpura associated with an autoantibody directed against a previously undefined platelet antigen. Blood 1987;69: 1458–1463.
- Minchinton RM, Cunningham I, Cole Sinclair M, Van der Weyden M, Vaughan S, McGrath KM: Autoreactive platelet antibody in post transfusion purpura. Aust NZ J Med 1990;20: 111–115.
- Porretti L, Marangoni F, Cofrancesco E, Neri MC, Rebulla P: Immunoglobulin classes and subclasses of platelet antibodies in a case of post–transfusion purpura (see comments). Vox Sang 1992;63:276–281.