- Polymorphonuclear leukocytes
- Innate immunity
- Mucosal immunity
- Fungal infections
- Bacterial infections
- Moraxella catarrhalis
- Staphylococcus aureus
- Haemophilus influenzae
- Candida albicans
Neutrophil-derived antimicrobial peptides and proteins (AMPs) play an important role in the defense against microbes. Absence of defense is illustrated by neutropenic patients with frequent bacterial and fungal infections. However, the specificity of the antimicrobial effects has not been adequately described. We set out to determine the specific antimicrobial pattern of polypeptides in neutrophils (polymorphonuclear leukocytes, PMNs) against 4 potential human pathogens: Moraxella catarrhalis, Staphylococcus aureus, Haemophilus influenzae and Candida albicans. Protein extracts of human PMNs were separated using high-performance liquid chromatography and fractions were assayed for antimicrobial activity. Fractions displaying antimicrobial activity were separated on SDS-PAGE and characterized using MALDI-MS. Depletion experiments were utilized to determine the contribution of each AMP to the antimicrobial effect. Among the identified AMPs, α-defensins 1–3, azurocidin, LL-37, lysozyme, calprotectin and lactotransferrin were studied in detail. We found a divergent pattern of killing, that is, certain peptides and proteins exhibited selective activity against specific pathogens, while others displayed a broader antimicrobial activity. α-Defensins, LL-37 and calprotectin were active against all species, while lactotransferrin exclusively inhibited growth of S. aureus. Conversely, azurocidin was active against all species except S. aureus. Our observations may shed light on bacterial resistance to AMPs and on the elimination of specific bacterial communities on mucosal surfaces.
Copyright © 2010 S. Karger AG, Basel
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Dr. Peter Bergman
Department of Laboratory Medicine, Division of Clinical Microbiology F68
Karolinska Institutet and Karolinska University Hospital, Huddinge
SE–14186 Stockholm (Sweden)
Tel. +46 8 5858 0000, Fax +46 8 5858 1305, E-Mail firstname.lastname@example.org
Received: April 20, 2010
Accepted after revision: June 22, 2010
Published online: September 4, 2010
Number of Print Pages : 14
Number of Figures : 6, Number of Tables : 2, Number of References : 44
Journal of Innate Immunity
Vol. 2, No. 6, Year 2010 (Cover Date: October 2010)
Journal Editor: Herwald H. (Lund), Egesten A. (Lund)
ISSN: 1662-811X (Print), eISSN: 1662-8128 (Online)
For additional information: http://www.karger.com/JIN
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