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Table of Contents
Vol. 33, No. 4, 2010
Issue release date: August 2010
Section title: Original Paper
Kidney Blood Press Res 2010;33:251–259

Maternal Protein Restriction Reduces Angiotensin II AT1 and AT2 Receptor Expression in the Fetal Rat Kidney

Alwasel S.H.a · Kaleem I.b · Sahajpal V.b · Ashton N.b
aDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; bFaculty of Life Sciences, University of Manchester, Manchester, UK
email Corresponding Author

Dr. Saleh Alwasel

Department of Zoology, College of Science, King Saud University

PO Box 2455

Riyadh 11451 (Saudi Arabia)

Tel. +966 0 5508 80848, E-Mail sawasel10@hotmail.com


  1. Barker DJ, Bagby SP: Developmental antecedents of cardiovascular disease: a historical perspective. J Am Soc Nephrol 2005;16:2537–2544.
  2. Langley SC, Jackson AA: Increased systolic pressure in adult rats induced by fetal exposure to maternal low protein diet. Clin Sci 1994;86:217–222.
  3. Manalich R, Reyes L, Herrera M, Melendi C, Fundora I: Relationship between weight at birth and the number and size of renal glomeruli in humans: a histomorphometric study. Kidney Int 2000;58:770–773.
  4. Hoy WE, Mathews JD, McCredie DA, Pugsley DJ, Hayhurst BG, Rees M, Kile E, Walker KA, Wang Z: The multidimensional nature of renal disease: rates and associations of albuminuria in an Australian Aboriginal community. Kidney Int 1998;54:1296–1304.
  5. Konje JC, Bell SC, Morton JJ, de Chazal R, Taylor DJ: Human fetal kidney morphometry during gestation and the relationship between weight, kidney morphometry and plasma active renin concentration at birth. Clin Sci 1996;91:169–175.
  6. Kingdom JC, Hayes M, McQueen J, Howatson AG, Lindop GB: Intrauterine growth restriction is associated with persistent juxtamedullary expression of renin in the fetal kidney. Kidney Int 1999;55:424–429.
  7. Langley-Evans SC, Welham SJ, Jackson AA: Fetal exposure to a maternal low protein diet impairs nephrogenesis and promotes hypertension in the rat. Life Sci 1999;64:965–974.
  8. Woods LL, Ingelfinger JR, Nyengaard JR, Rasch R: Maternal protein restriction suppresses the newborn renin-angiotensin system and programs adult hypertension in rats. Pediatr Res 2001;49:460–467.
  9. Sahajpal V, Ashton N: Renal function and angiotensin AT1 receptor expression in young rats following intrauterine exposure to a maternal low-protein diet. Clin Sci (Lond) 2003;104:607–614.
  10. Sahajpal V, Ashton N: Increased glomerular angiotensin II binding in rats exposed to a maternal low protein diet in utero. J Physiol 2005;563:193–201.
  11. Mao C, Wu J, Xiao D, Lv J, Ding Y, Xu Z, Zhang L: The effect of fetal and neonatal nicotine exposure on renal development of AT1 and AT2 receptors. Reprod Toxicol 2009;27:149–154.
  12. Samuelsson AM, Alexanderson C, Molne J, Haraldsson B, Hansell P, Holmang A: Prenatal exposure to interleukin-6 results in hypertension and alterations in the renin-angiotensin system of the rat. J Physiol 2006;575:855–867.
  13. McMullen S, Langley-Evans SC: Sex-specific effects of prenatal low-protein and carbenoxolone exposure on renal angiotensin receptor expression in rats. Hypertension 2005;46:1374–1380.
  14. Singh RR, Cullen-McEwen LA, Kett MM, Boon WM, Dowling J, Bertram JF, Moritz KM: Prenatal corticosterone exposure results in altered AT1/AT2, nephron deficit and hypertension in the rat offspring. J Physiol 2007;579:503–513.
  15. Aguilera G, Kapur S, Feuillan P, Sunar-Akbasak B, Bathia AJ: Developmental changes in angiotensin II receptor subtypes and AT1 receptor mRNA in rat kidney. Kidney Int 1994;46:973–979.
  16. Norwood VF, Craig MR, Harris JM, Gomez RA: Differential expression of angiotensin II receptors during early renal morphogenesis. Am J Physiol 1997;272:R662–R668.
  17. Yamada T, Horiuchi M, Dzau VJ: Angiotensin II type 2 receptor mediates programmed cell death. Proc Natl Acad Sci USA 1996;93:156–160.
  18. Wolf G, Ziyadeh FN, Zahner G, Stahl RA: Angiotensin II is mitogenic for cultured rat glomerular endothelial cells. Hypertension 1996;27:897–905.
  19. Ray PE, Aguilera G, Kopp JB, Horikoshi S, Klotman PE: Angiotensin II receptor-mediated proliferation of cultured human fetal mesangial cells. Kidney Int 1991;40:764–771.
  20. Hellmann H, Davis JM, Thurau K: Blood pressure and indices of glomerular filtration area in hypertensive and normotensive Prague rats. Kidney Blood Press Res 2000;23:385–392.
  21. Souster LP, Emery JL: The sizes of renal glomeruli in fetuses and infants. J Anat 1980;130:595–602.
  22. Garcia-Villalba P, Denkers ND, Wittwer CT, Hoff C, Nelson RD, Mauch TJ: Real-time PCR quantification of AT1 and AT2 angiotensin receptor mRNA expression in the developing rat kidney. Nephron Exp Nephrol 2003;94:e154–e159.
  23. Tufro-McReddie A, Harrison JK, Everett AD, Gomez RA: Ontogeny of type 1 angiotensin II receptor gene expression in the rat. J Clin Invest 1993;91:530–537.
  24. Moritz KM, Johnson K, Douglas-Denton R, Wintour EM, Dodic M: Maternal glucocorticoid treatment programs alterations in the renin-angiotensin system of the ovine fetal kidney. Endocrinology 2002;143:4455–4463.
  25. Gilbert JS, Ford SP, Lang AL, Pahl LR, Drumhiller MC, Babcock SA, Nathanielsz PW, Nijland MJ: Nutrient restriction impairs nephrogenesis in a gender-specific manner in the ovine fetus. Pediatr Res 2007;61:42–47.
  26. Zohdi V, Moritz KM, Bubb KJ, Cock ML, Wreford N, Harding R, Black MJ: Nephrogenesis and the renal renin-angiotensin system in fetal sheep: effects of intrauterine growth restriction during late gestation. Am J Physiol Regul Integr Comp Physiol 2007;293:R1267–1273.
  27. Nishimura H, Yerkes E, Hohenfellner K, Miyazaki Y, Ma J, Hunley TE, Yoshida H, Ichiki T, Threadgill D, Phillips JA 3rd, Hogan BM, Fogo A, Brock JW 3rd, Inagami T, Ichikawa I: Role of the angiotensin type 2 receptor gene in congenital anomalies of the kidney and urinary tract, CAKUT, of mice and men. Mol Cell 1999;3:1–10.
  28. Welham SJ, Wade A, Woolf AS: Protein restriction in pregnancy is associated with increased apoptosis of mesenchymal cells at the start of rat metanephrogenesis. Kidney Int 2002;61:1231–1242.
  29. Vehaskari VM, Aviles DH, Manning J: Prenatal programming of adult hypertension in the rat. Kidney Int 2001;59:238–245.
  30. Wolf G, Ziyadeh FN, Helmchen U, Zahner G, Schroeder R, Stahl RA: Ang II is a mitogen for a murine cell line isolated from medullary thick ascending limb of Henle’s loop. Am J Physiol 1995;268:F940–F947.
  31. Gibbons GH, Pratt RE, Dzau VJ: Vascular smooth muscle cell hypertrophy vs. hyperplasia: autocrine transforming growth factor-β1 expression determines growth response to angiotensin II. J Clin Invest 1992;90:456–461.
  32. Spence SG, Allen HL, Cukierski MA, Manson JM, Robertson RT, Eydelloth RS: Defining the susceptible period of developmental toxicity for the AT1-selective angiotensin II receptor antagonist losartan in rats. Teratology 1995;51:367–382.
  33. Oliverio MI, Kim HS, Ito M, Le T, Audoly L, Best CF, Hiller S, Kluckman K, Maeda N, Smithies O, Coffman TM: Reduced growth, abnormal kidney structure, and type 2 (AT2) angiotensin receptor-mediated blood pressure regulation in mice lacking both AT1a and AT1b receptors for angiotensin II. Proc Natl Acad Sci USA 1998;95:15496–15501.
  34. Tufro-McReddie A, Romano LM, Harris JM, Ferder L, Gomez RA: Angiotensin II regulates nephrogenesis and renal vascular development. Am J Physiol 1995;269:F110–F115.
  35. Merlet-Benichou C, Gilbert T, Muffat-Joly M, Lelievre-Pegorier M, Leroy B: Intrauterine growth retardation leads to a permanent nephron deficit in the rat. Pediatr Nephrol 1994;8:175–180.
  36. Langley-Evans SC, Gardner DS, Jackson AA: Association of disproportionate growth of fetal rats in late gestation with raised systolic blood pressure in later life. J Reprod Fertil 1996;106:307–312.
  37. Luyckx VA, Compston CA, Simmen T, Mueller TF: Accelerated senescence in kidneys of low birth weight rats after catch-up growth. Am J Physiol Renal Physiol 2009.