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Vol. 86, No. 2, 2011
Issue release date: March 2011
Section title: Original Paper
Urol Int 2011;86:191–196
(DOI:10.1159/000319366)

Mesenchymal Stem Cells Ameliorate Ischemia-Reperfusion-Induced Renal Dysfunction by Improving the Antioxidant/Oxidant Balance in the Ischemic Kidney

Zhuo W. · Liao L. · Xu T. · Wu W. · Yang S. · Tan J.
Organ Transplant Institute, Fuzhou General Hospital, Xiamen University, Fuzhou, PR China

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Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 3/15/2010
Accepted: 7/7/2010
Published online: 9/28/2010

Number of Print Pages: 6
Number of Figures: 2
Number of Tables: 3

ISSN: 0042-1138 (Print)
eISSN: 1423-0399 (Online)

For additional information: http://www.karger.com/UIN

Abstract

Background: Renal ischemia followed by reperfusion leads to acute renal failure in both native kidneys and renal allograft. This study aimed at investigating the effects ofmesenchymal stem cells (MSC) on ischemia/reperfusion (I/R) injury and the underlying mechanisms in a rat model. Methods: Renal ischemia was produced by clamping the right renal vessels for 60 min after the left kidney was removed. Immediately after visual confirmation of reflow, 1 × 106 MSC were administered by intravenous injection, followed by reperfusion for 24 h. The kidney functions, tissue malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels were evaluated. Histopathological examinations were also performed. Results: MSC infusion significantly improved kidney function as indicated by lower urea and creatinine levels in the MSC compared to the vehicle group (p < 0.05). I/R-induced reduction in renal tissue SOD enzyme activity and GSH-Px was significantly improved by MSC (p < 0.05). Treatment with MSC also resulted in significant reduction in renal tissue MDA levels that were increased by renal I/R injury (p < 0.05). At histological examination, the kidneys of MSC-treated rats showed a fairly normal morphology. Conclusions: MSC protects the kidneys against I/R injury at least via their antioxidant effects.


Article / Publication Details

First-Page Preview
Abstract of Original Paper

Received: 3/15/2010
Accepted: 7/7/2010
Published online: 9/28/2010

Number of Print Pages: 6
Number of Figures: 2
Number of Tables: 3

ISSN: 0042-1138 (Print)
eISSN: 1423-0399 (Online)

For additional information: http://www.karger.com/UIN


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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References

  1. Abernethy VE, Lieberthal W: Acute renal failure in the critically ill patient. Crit Care Clin 2002;18:203–222.
  2. Hertig A, Verine J, Mougenot B, Jouanneau C, Ouali N, Sebe P, Glotz D, Ancel PY, Rondeau E, Xu-Dubois YC: Risk factors for early epithelial to mesenchymal transition in renal grafts. Am J Transplant 2006;6:2937–2946.
  3. Tanabe K, Oshima T, Tokumoto T, Ishikawa N, Kanematsu A, Shinmura H, Koga S, Fuchinoue S, Takahashi K, Toma H: Long-term renal function in on-heart-beating donor kidney transplantation: a single-center experience. Transplantation 1998;66:1708–1713.
  4. Perico N, Cattaneo D, Sayegh MH, Remuzzi G: Delayed graft function in kidney transplantation. Lancet 2004;364:1814–1827.
  5. Haugen E, Nath KA: The involvement of oxidative stress in the progression of renal injury. Blood Purif 1999;17:58–65.
  6. Edelstein CL, Ling H, Schrier RW: The nature of cell injury. Kidney Int 1997;51:1341–1351.
  7. Castaneda MP, Swiatecka-Urban A, Mitsnefes MM, Feuerstein D, Kaskel FJ, Tellis V, Devarajan P: Activation of mitochondrial apoptotic pathways in human, renal allografts after ischemia reperfusion injury. Transplantation 2003;76:50–54.
  8. Thadhani R, Pascual M, Bonventre JV: Acute renal failure. N Engl J Med 1996;334:1448–1460.
  9. Elly KJ, Molitoris BA: Acute renal failure in the new millennium: time to consider combination therapy. Semin Nephrol 2000;20:4–19.

    External Resources

  10. Yokoo T, Sakurai K, Ohashi T, Kawamura T: Stem cell gene therapy for chronic renal failure. Curr Gene Ther 2003;3:387–394.
  11. Grove JE, Bruscia E, Krause DS: Plasticity of bone marrow-derived stem cells. Stem Cells2004;22:487–500.
  12. Herzog EL, Chai L, Krause DS: Plasticity of marrow-derived stem cells. Blood 2003;102:3483–3493.
  13. Krause DS: Plasticity of marrow-derived stem cells. Gene Ther2002;9:754–758.
  14. Lange C, Tögel F, Ittrich H, Clayton F, Nolte-Ernsting C, Zander AR, Westenfelder C: Administered mesenchymal stem cells enhance recovery from ischemia/reperfusion-induced acute renal failure in rats. Kidney Int 2005;68:1613–1617.
  15. Gupta S, Verfaillie C, Chmielewski D, Kim Y, Rosenberg ME: A role for extra-renal cells in the regeneration following acute renal failure. Kidney Int 2002;62:1285–1290.
  16. Duffield JS, Park KM, Hsiao LL, Kelley VR, Scadden DT, Ichimura T, Bonventre JV: Restoration of tubular epithelial cells during repair of the post-ischemic kidney occurs independently of bone marrow-derived stem cells. J Clin Invest 2005;115:1743–1755.
  17. Kale S, Karihaloo A, Clark PR, Kashgarian M, Krause DS, Cantley LG: Bone marrow stem cells contribute to repair of the ischemically injured renal tubule. J Clin Invest 2003;112:42–49.
  18. Morigi M, Imberti B, Zoja C, Corna D, Tomasoni S, Abbate M, Rottoli D, Angioletti S, Benigni A, Perico N, Alison M, Remuzzi G: Mesenchymal stem cells are renotropic, helping to repair the kidney and improve function in acute renal failure. J Am Soc Nephrol 2004;15:1794–1804.
  19. Ögel F, Hu Z, Weiss K, Isaac J, Lange C, Westenfelder C: Administered mesenchymal stem cells protect against ischemic acute renal failure through differentiation-independent mechanisms. Am J Physiol Renal Physiol 2005;289:F31–F42.
  20. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR: Multi-lineage potential of adult human mesenchymal stem cells. Science 1999;284:143–147.
  21. Esterbauer H, Cheeseman KH: Determination of aldehydiclipid peroxidation products: malonaldehyde and 4-hydroxynonenal; in Packer L, Glazer AN (eds): Oxygen Radicals in Biological Systems. Methods in Enzymology. San Diego, Academic Press, 1990, pp 407–421.
  22. Bayrak O, Uz E, Bayrak R, Turgut F, Atmaca AF, Sahin S, Yildirim ME, Kaya A, Cimentepe E, Akcay A: Curcumin protects against ischemia/reperfusion injury in rat kidneys. World J Urol 2008;26:285–291.
  23. Williams PH, Lopez DB, Chan C, Ezrin A, Hottendorf R: Characterization of renal ischemia-reperfusion injury in rats. J Pharmacol Toxicol Methods 1997;37:1–7.
  24. Weight SC, Bell PRF, Nicholson ML: Renal ischemia reperfusion injury. Br J Surg 1996;83:162–170.
  25. Paller MS, Hoidal JR, Ferris TF: Oxygen free radicals in ischemic acute renal failure in the rat. J Clin Invest 1984;74:1156–1164.
  26. Eschwege P, Paradis V, Conti M, Holstege A, Richet F, Deteve J,Menager P, Legrand A, Jardin A, Bedossa P, Benoit G: In situ detection of lipid peroxidation by-products as markers of renal ischemia injuries in rat kidneys. J Urol 1999;162:553–557.
  27. Zhang Z, Dmitrieva NI, Park JH, Levine RL, Burg MB: High urea and NaCl carbonylate proteins in renal cells in culture and in vivo, and high urea causes 8-oxoguanine lesions in their DNA. Proc Natl Acad Sci USA 2004;101:9491–9496.
  28. Mberti B, Morigi M, Tomasoni S, Rota C, Corna D, Longaretti L, Rottoli D, Valsecchi F, Benigni A, Wang J, Abbate M, Zoja C, Remuzzi G: Insulin-like growth factor-1 sustains stem cell mediated renal repair. J Am Soc Nephrol 2007;18:2921–2928.
  29. Umino Y, Naoaki I: Hyperoxia exposure induced hormesis decreases mitochondrial superoxide radical levels via Ins/IGF-1 signaling pathway in a long-lived age-1 mutant of Caenorhabditis elegans. J Radiat Res 2008;49:211–218.
  30. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science 1999;284:143–147.