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Table of Contents
Vol. 3, No. 1, 2011
Issue release date: December 2010
Section title: Short Communication
J Innate Immun 2011;3:65–70

Clotting Factors and Eicosanoids Protect against Nematode Infections

Hyrsl P.a, b · Dobes P.a, b · Wang Z.a · Hauling T.a · Wilhelmsson C.a · Theopold U.a
aDepartment of Molecular Biology and Functional Genomics, Stockholm University, Stockholm, Sweden; bDepartment of Animal Physiology and Immunology, Institute of Experimental Biology, Masaryk University, Brno, Czech Republic
email Corresponding Author

Dr. Ulrich Theopold

Department of Molecular Biology and Functional Genomics

University of Stockholm

SE–10691 Stockholm (Sweden)

Tel. +46 816 4181, Fax +46 816 6488, E-Mail uli@molbio.su.se


  1. Theopold U, Dushay MS: Mechanisms of Drosophila immunity – an innate immune system at work. Curr Immunol Rev 2007;3:276–288.
  2. Kawabata S, Koshiba T, Shibata T: The lipopolysaccaride-activated innate immune response network of the horseshoe crab. Invertebr Surv J 2009;6:59–77.
  3. Theopold U, Li D, Fabbri M, Scherfer C, Schmidt O: The coagulation of insect hemolymph. Cell Mol Life Sci 2002;59:363–372.
  4. Scherfer C, Karlsson C, Loseva O, et al: Isolation and characterization of hemolymph clotting factors in Drosophila melanogaster by a pullout method. Curr Biol 2004;14:625–629.
  5. Karlsson C, Korayem AM, Scherfer C, Loseva O, Dushay MS, Theopold U: Proteomic analysis of the Drosophila larval hemolymph clot. J Biol Chem 2004;279:52033–52041.
  6. Wang Z, Wilhelmsson C, Hyrsl P, et al: Pathogen entrapment by transglutaminase – a conserved early innate immune mechanism. PLoS Pathog 2010;6:e1000763.
    External Resources
  7. Ffrench-Constant R, Waterfield N, Daborn P, et al: Photorhabdus: towards a functional genomic analysis of a symbiont and pathogen. FEMS Microbiol Rev 2003;26:433–456.
  8. Hallem EA, Rengarajan M, Ciche TA, Sternberg PW: Nematodes, bacteria, and flies: a tripartite model for nematode parasitism. Curr Biol 2007;17:898–904.
  9. Simmons DL, Botting RM, Hla T: Cyclooxygenase isozymes: the biology of prostaglandin synthesis and inhibition. Pharmacol Rev 2004;56:387–437.
  10. Dietzl G, Chen D, Schnorrer F, et al: A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 2007;448:151–156.
  11. Korayem AM, Fabbri M, Takahashi K, et al: A Drosophila salivary gland mucin is also expressed in immune tissues: evidence for a function in coagulation and the entrapment of bacteria. Insect Biochem Mol Biol 2004;34:1297–1304.
  12. Scherfer C, Qazi MR, Takahashi K, et al: The Toll immune-regulated Drosophila protein Fondue is involved in hemolymph clotting and puparium formation. Dev Biol 2006;295:156–163.
  13. Lindgren M, Riazi R, Lesch C, Wilhelmsson C, Theopold U, Dushay MS: Fondue and transglutaminase in the Drosophila larval clot. J Insect Physiol 2008;54:586–592.
  14. Bidla G, Lindgren M, Theopold U, Dushay MS: Hemolymph coagulation and phenoloxidase in Drosophila larvae. Dev Comp Immunol 2005;29:669–679.
  15. Theopold U, Schmidt O: Helix pomatia lectin and annexin V, two molecular probes for insect microparticles: possible involvement in hemolymph coagulation. J Insect Physiol 1997;43:667–674.
  16. Stanley D, Miller J, Tunaz H: Eicosanoid actions in insect immunity. J Innate Immun 2009;1:282–290.
  17. Shrestha S, Park Y, Stanley D, Kim Y: Genes encoding phospholipases A2 mediate insect nodulation reactions to bacterial challenge. J Insect Physiol 2010;56:324–332.
  18. Kim Y, Ji D, Cho S, Park Y: Two groups of entomopathogenic bacteria, Photorhabdus and Xenorhabdus, share an inhibitory action against phospholipase A2 to induce host immunodepression. J Invertebr Pathol 2005;89:258–264.
  19. Shrestha S, Kim Y: Biochemical characteristics of immune-associated phospholipase A(2) and its inhibition by an entomopathogenic bacterium, Xenorhabdus nematophila. J Microbiol 2009;47:774–782.
  20. Stramer B, Winfield M, Shaw T, Millard TH, Woolner S, Martin P: Gene induction following wounding of wild-type versus macrophage-deficient Drosophila embryos. EMBO Rep 2008;9:465–471.
  21. Yajima M, Takada M, Takahashi N, et al: A newly established in vitro culture using transgenic Drosophila reveals functional coupling between the phospholipase A2-generated fatty acid cascade and lipopolysaccharide-dependent activation of the immune deficiency (imd) pathway in insect immunity. Biochem J 2003;371:205–210.
  22. Aymeric JL, Givaudan A, Duvic B: Imd pathway is involved in the interaction of Drosophila melanogaster with the entomopathogenic bacteria, Xenorhabdusnematophila and Photorhabdus luminescens. Mol Immunol 2010;47:2342–2348.
  23. Rizki RM, Rizki TM: Encapsulation of parasitoid eggs in phenoloxidase-deficient mutants of Drosophilamelanogaster. J Insect Physiol 1990;36:523–529.
  24. Braun A, Hoffmann JA, Meister M: Analysis of the Drosophila host defense in domino mutant larvae, which are devoid of hemocytes. Proc Natl Acad Sci USA 1998;95:14337–14342.
  25. Park Y, Kim Y: Xenorhabdus nematophilus inhibits p-bromophenacyl bromide (BPB)-sensitive PLA2 of Spodoptera exigua. Arch Insect Biochem Physiol 2003;54:134–142.
  26. Kubata BK, Duszenko M, Martin KS, Urade Y: Molecular basis for prostaglandin production in hosts and parasites. Trends Parasitol 2007;23:325–331.