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J Innate Immun 2011;3:150–166

Activation of Pulmonary Dendritic Cells and Th2-Type Inflammatory Responses on Instillation of Engineered, Environmental Diesel Emission Source or Ambient Air Pollutant Particles in vivo

Bezemer G.F.G.a · Bauer S.M.b · Oberdörster G.c, e · Breysse P.N.f · Pieters R.H.H.a · Georas S.N.b, d, e · Williams M.A.b, d, e
aImmunotoxicology Institute for Risk Assessment Sciences, Utrecht University, Utrecht, The Netherlands; bDivision of Pulmonary and Critical Care Medicine, cDivision of Respiratory Biology and Toxicology, dLung Biology and Disease Program, eDepartment of Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, N.Y., and fDepartment of Environmental Health Sciences, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Md., USA
email Corresponding Author

 goto top of outline Key Words

  • Innate immunity
  • Allergic immunity
  • Dendritic cell
  • Lung
  • Inflammation
  • Immunotoxicology
  • Toxicology
  • Particulate matter
  • Nanoparticles

 goto top of outline Abstract

The biological effects of acute particulate air pollution exposure in host innate immunity remain obscure and have relied largely on in vitro models. We hypothesized that single acute exposure to ambient or engineered particulate matter (PM) in the absence of other secondary stimuli would activate lung dendritic cells (DC) in vivo and provide information on the early immunological events of PM exposure and DC activation in a mouse model naïve to prior PM exposure. Activation of purified lung DC was studied following oropharyngeal instillation of ambient particulate matter (APM). We compared the effects of APM exposure with that of diesel-enriched PM (DEP), carbon black particles (CBP) and silver nanoparticles (AgP). We found that PM species induced variable cellular infiltration in the lungs and only APM exposure induced eosinophilic infiltration. Both APM and DEP activated pulmonary DC and promoted a Th2-type cytokine response from naïve CD4+ T cells ex vivo. Cultures of primary peribronchial lymph node cells from mice exposed to APM and DEP also displayed a Th2-type immune response ex vivo. We conclude that exposure of the lower airway to various PM species induces differential immunological responses and immunomodulation of DC subsets. Environmental APM and DEP activated DC in vivo and provoked a Th2 response ex vivo. By contrast, CBP and AgP induced altered lung tissue barrier integrity but failed to stimulate CD4+ T cells as effectively. Our work suggests that respirable pollutants activate the innate immune response with enhanced DC activation, pulmonary inflammation and Th2-immune responsiveness.

Copyright © 2010 S. Karger AG, Basel

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 goto top of outline Author Contacts

Dr. Marc Adrian Williams
NHEERL, Environmental Public Health Division, Cardiopulmonary and Immunotoxicology Branch, Office of Research and Development
US Environmental Protection Agency, Research Triangle Park, NC 27711 (USA)
Tel. +1 919 541 2255, E-Mail

 goto top of outline Article Information

Received: August 16, 2010
Accepted after revision: October 6, 2010
Published online: November 23, 2010
Number of Print Pages : 17
Number of Figures : 7, Number of Tables : 1, Number of References : 65

 goto top of outline Publication Details

Journal of Innate Immunity

Vol. 3, No. 2, Year 2011 (Cover Date: February 2011)

Journal Editor: Herwald H. (Lund), Egesten A. (Lund)
ISSN: 1662-811X (Print), eISSN: 1662-8128 (Online)

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