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J Mol Microbiol Biotechnol 2010;19:231–241

Functional Analysis of the p40 and p75 Proteins from Lactobacillus casei BL23

Bäuerl C.a · Pérez-Martínez G.a · Yan F.b · Polk D.B.c · Monedero V.a
aLaboratorio de Bacterias Lácticas y Probióticos, Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Burjassot, Valencia, Spain; bDepartment of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Vanderbilt University School of Medicine and the Monroe Carell Jr. Children’s Hospital at Vanderbilt, Nashville, Tenn., and cDepartment of Pediatrics, University of Southern California and Children’s Hospital Los Angeles, Los Angeles, Calif., USA
email Corresponding Author

 goto top of outline Key Words

  • Lactobacillus
  • Probiotic
  • Cell-wall hydrolases

 goto top of outline Abstract

The genomes of Lactobacillus casei/paracasei and Lactobacillus rhamnosus strains carry two genes encoding homologues of p40 and p75 from L. rhamnosus GG, two secreted proteins which display anti-apoptotic and cell protective effects on human intestinal epithelial cells. p40 and p75 carry cysteine, histidine-dependent aminohydrolase/peptidase (CHAP) and NLPC/P60 domains, respectively, which are characteristic of proteins with cell-wall hydrolase activity. In L. casei BL23 both proteins were secreted to the growth medium and were also located at the bacterial cell surface. The genes coding for both proteins were inactivated in this strain. Inactivation of LCABL_00230 (encoding p40) did not result in a significant difference in phenotype, whereas a mutation in LCABL_02770 (encoding p75) produced cells that formed very long chains. Purified glutathione-S-transferase (GST)-p40 and -p75 fusion proteins were able to hydrolyze the muropeptides from L. casei cell walls. Both fusions bound to mucin, collagen and to intestinal epithelial cells and, similar to L. rhamnosus GG p40, stimulated epidermal growth factor receptor phosphorylation in mouse intestine ex vivo. These results indicate that extracellular proteins belonging to the machinery of cell-wall metabolism in the closely related L. casei/paracasei-L. rhamnosus group are most likely involved in the probiotic effects described for these bacteria

Copyright © 2010 S. Karger AG, Basel

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 goto top of outline Author Contacts

Vicente Monedero
Laboratorio de Bacterias Lácticas y Probióticos
Instituto de Agroquímica y Tecnología de Alimentos (CSIC)
PO Box 73, ES–46100 Burjassot, Valencia (Spain)
Tel. +34 963 900 022, Fax +34 963 636 301, E-Mail

 goto top of outline Article Information

Published online: December 17, 2010
Number of Print Pages : 11
Number of Figures : 8, Number of Tables : 1, Number of References : 39

 goto top of outline Publication Details

Journal of Molecular Microbiology and Biotechnology

Vol. 19, No. 4, Year 2010 (Cover Date: January 2011)

Journal Editor: Saier Jr. M.H. (La Jolla, Calif.)
ISSN: 1464-1801 (Print), eISSN: 1660-2412 (Online)

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