Objective: The present study was carried out to investigate the antinociceptive and anti-inflammatory activities of virgin coconut oil (VCO) produced by theMalaysian Agriculture Research and Development Institute (MARDI) using various in vivo models. Materials and Methods: Two types of VCOs, produced via standard drying (VCOA) and fermentation (VCOB) processes were used in this study. Both VCOA and VCOB were serially diluted using 1% Tween 80 to concentrations (v/v) of 10, 50 and 100%. Antinociceptive and anti- inflammatory activities of both VCOs were examined using various in vivomodel systems. The antinociceptive activity of the VCOs were compared to those of 1% Tween 80 (used as a negative control), morphine (5 mg/kg) and/or acetylsalicylic acid (100 mg/kg). Results: Both VCOA and VCOB exhibited significant (p < 0.05) dose-dependent antinociceptive activity in the acetic acid-induced writhing test. Both VCOs also exerted significant (p < 0.05) antinociceptive activity in both phases of the formalin and hot-plate tests. Interestingly, the VCOs exhibited anti-inflammatory activity in an acute (carrageenan-induced paw edema test), but not in a chronic (cotton-pellet-induced granuloma test) model of inflammation. Conclusion: The MARDI-produced VCOs possessed antinociceptive and anti-inflammatory activities. Further studies are needed to confirm these observations.
© 2011 S. Karger AG, Basel
- Virgin coconut oil
- Antinociceptive activity
- Anti-inflammatory activity
- Zakaria ZA, Reezal I, Mat Jais AM, Marmin AHI, Sidek H, Husin SH, Rahim MHA, Sabtu L, Somchit MN, Sulaiman MR: The anti-inflammatory, antipyretic and wound healing activities of Cocos nucifera (MATAG Types) fresh juice and kernel extracts in experimental animals. J Pharmacol Toxicol 2006;1:516–526.
- Marina AM, Che Man YB, Nazimah AH: Chemical properties of virgin coconut oil. J Am Oil Chem Soc 2009;86:301–307.
- Nevin KG, Rajamohan T: Beneficial effects of virgin coconut oil on lipid parameters and in vitro LDL oxidation. Clin Biochem 2004;37:830–835.
- Nevin KG, Rajamohan T: Influence of virgin coconut oil on blood coagulation factors, lipid levels and LDL oxidation in cholesterol fed Sprague-Dawley rats. Eur J Clin Nutr Metab 2008;3:1–8.
- Nevin KG, Rajamohan T: Virgin coconut oil supplemented diet increases the antioxidant status in rats. Food Chem 2006;99:260–266.
- Zimmermann M: Ethical guidelines for investigations of experimental pain in conscious animals. Pain 1983;16:109–110.
- Zakaria ZA, Raden Mohd Nor RNS, Hanan Kumar G, Abdul Ghani ZDF, Sulaiman MR, Rathna Devi G, Mat Jais AM, Somchit MN, Fatimah CA: Antinociceptive, anti-inflammatory and anti-pyretic properties of Melastoma malabathricum leaves aqueous extract in experimental animals. Can J Physiol Pharmacol 2006;84:1291–1299.
- Zakaria ZA, Hanan Kumar G, Zainal H, Mohd Pojan NH, Morsid NA, Aris A, Sulaiman MR: Antinociceptive, anti-inflammatory and antipyretic effects of Solanum nigrum chloroform extract in animal models. Yakugaku Zasshi 2006;126:1171–1178.
- Zakaria ZA, Hanan Kumar G, Raden Mohd Nor RNS, Sulaiman MR, Fatimah CA, Mat Jais AM, Somchit MN, Ismail MS: Antinociceptive, anti-inflammatory and antipyretic properties of Corchorus capsularis leaves aqueous extract in experimental animal models. Pharm Biol 2009;47:104–110.
- Zakaria ZA, Sulaiman MR, Hanan Kumar G, Abdul Ghani ZDF, Raden Mohd Nor RNS, Rathna Devi G, Mat Jais AM, Fatimah CA: Antinociceptive and anti-inflammatory properties of Corchorus capsularis leaves chloroform extract in experimental animal models. Yakugaku Zasshi 2007;127:359–365.
- Sulaiman MR, Zakaria ZA, Chiong HS, Lai SK, Israf DA, Tg Azam Shah TM: Antinociceptive and anti-inflammatory effects of Stachytarpheta jamaicensis (L.) Vahl. (Verbenaceae) in experimental animal models. Med Princ Pract 2009;18:272–279.
- Bentley GA, Newton SH, Starr J: Evidence for an action of morphine and the enkephalins on sensory nerve endings in the mouse peritoneum. Br J Pharmacol 1981;73:325–332.
- Pini LA, Vitale G, Ottani A, Sandrini M: Naloxone-reversible antinociception by paracetamol in the rat. J Pharmacol Exp Ther 1997;280:934–940.
- Zakaria ZA, Hanan Kumar G, Mat Jais AM, Sulaiman MR, Somchit MN: Antinociceptive, anti-inflammatory and antipyretic properties of Channa striatus fillet aqueous and lipid-based extracts in rats. Methods Find Exp Clin Pharmacol 2008;30:355–362.
- Winter CA, Risley EA, Nuss GW: Carrageenan-induced edema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Biol Med 1962;111:544–547.
- Kahn MA, Solomon LW: Acute Inflammation/Wound Healing and Repair II. http://ocw.tufts.edu/data/51/551543.pdf. 2007.
- Dos Santos MD, Almeida MC, Lopes NP, de Souza GEP: Evaluation of the anti-inflammatory, analgesic and antipyretic activities of the natural polyphenol chlorogenic acid. Biol Pharm Bull 2006;29:2236–2240.
- Küpeli E, Pinar SF, Ihsan C, Yesilada E, Ezer N: Phenolic compounds of Sideritis ozturkii and their in vivo anti-inflammatory and antinociceptive activities. J Ethnopharmacol 2007;112:356–360.
- Natarajan K, Singh S, Burke TR, Grunberger D, Aggarwal BB: Caffeic acid phenethyl ester is a potent and specific inhibitor of activation of nuclear transcription factor NF-kappa B. Proc Natl Acad Sci USA 1996;93:9090–9095.
- Zakaria ZA, Ahmad Z, Somchit MN, Arifah AK, Sulaiman MR, Teh LK, Salleh MZ, Long K: Antihypercholesterolemia property and fatty acid composition of MARDI-produced virgin coconut oils. Afr J Pharm Pharmacol 2010;4:636–644.
- Zakaria ZA, Sulaiman MR, Goh YM, Mat Jais AM, Somchit MN: Determination of the amino acid and fatty acid compositions of the aqueous extract of Channa striatus (Haruan) that exhibits antinociceptive activity. Clin Exp Pharmacol Physiol 2007;34:198–204.
Dr. Zainul Amiruddin Zakaria
Department of Biomedical Science, Faculty of Medicine and Health Sciences
Universiti Putra Malaysia
43400 UPM Serdang, Selangor (Malaysia)
Tel. +60 3 8947 2654, Fax +60 3 8943 6178, E-Mail firstname.lastname@example.org
Received: March 5, 2010
Accepted: October 13, 2010
Published online: March 29, 2011
Number of Print Pages : 6
Number of Figures : 0, Number of Tables : 4, Number of References : 21
Medical Principles and Practice (International Journal of the Kuwait University Health Sciences Centre)
Vol. 20, No. 3, Year 2011 (Cover Date: March 2011)
Journal Editor: Owunwanne A. (Kuwait), Benov L. (Kuwait)
ISSN: 1011-7571 (Print), eISSN: 1423-0151 (Online)
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