Journal Mobile Options
Table of Contents
Vol. 58, No. 3, 2011
Issue release date: August 2011
Ann Nutr Metab 2011;58:250–258

Catechin-Rich Grape Seed Extract Supplementation Attenuates Diet-Induced Obesity in C57BL/6J Mice

Ohyama K. · Furuta C. · Nogusa Y. · Nomura K. · Miwa T. · Suzuki K.
Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan

Individual Users: Register with Karger Login Information

Please create your User ID & Password

Contact Information

I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in


Background: Grape seed extracts (GSE) are known to present health benefits such as antioxidative and anti-obesity effects in animal models. The purpose of this research is to determine whether the specially manufactured GSE, catechin-rich GSE (CGSE), can protect against obesity induced by a high-fat diet (HFD) and to address the mechanism underlying this effect. Methods: The componential analysis of CGSE was performed using liquid chromatography/mass spectrometry. Oxygen consumption and the respiratory quotient were determined using 500 mg/kg CGSE administered orally for 3 days in 14- to 15-week-old male C57BL/6J mice. Nine-week-old male C57BL/6J mice were supplemented with 0.5 or 1% CGSE in a HFD for 12 weeks, and their body weight and food intake were monitored. Blood and tissue samples were collected and analyzed. Results: The main polyphenol components of CGSE were catechin and epicatechin. CGSE supplementation in the HFD-induced obesity model chronically suppressed the increase in body weight and the weight of fat pads. Furthermore, CGSE improved metabolic parameter abnormalities and upregulated the fatty acid oxidation-related genes in the liver. Conclusions: These findings suggest that CGSE contains monomeric catechins in high concentrations and ameliorates HFD-induced obesity in C57BL/6J mice.

Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.


  1. Lamarche B, Lemieux S, Dagenais GR, Despres JP: Visceral obesity and the risk of ischaemic heart disease: insights from the Québec Cardiovascular Study. Growth Horm IGF Res 1998;8(suppl B):1–8.

    External Resources

  2. Matsuzawa Y: The metabolic syndrome and adipocytokines. FEBS Lett 2006;580:2917–2921.
  3. Monagas M, Gomez-Cordoves C, Bartolome B, Laureano O, Ricardo da Silva JM: Monomeric, oligomeric, and polymeric flavan-3-ol composition of wines and grapes from Vitis vinifera L. Cv. Graciano, Tempranillo, and Cabernet Sauvignon. J Agric Food Chem 2003;51:6475–6481.
  4. Soleas GJ, Diamandis EP, Goldberg DM: Wine as a biological fluid: history, production, and role in disease prevention. J Clin Lab Anal 1997;11:287–313.
  5. Tsai Su C, Singleton VL: Identification of three flavan-3-ols from grapes. Phytochemistry 1969;8:1553–1558.

    External Resources

  6. Koga T, Moro K, Nakamori K, Yamakoshi J, Hosoyama H, Kataoka S, Ariga T: Increase of antioxidative potential of rat plasma by oral administration of proanthocyanidin-rich extract from grape seeds. J Agric Food Chem 1999;47:1892–1897.
  7. Yamakoshi J, Kataoka S, Koga T, Ariga T: Proanthocyanidin-rich extract from grape seeds attenuates the development of aortic atherosclerosis in cholesterol-fed rabbits. Atherosclerosis 1999;142:139–149.
  8. Yokozawa T, Kim HJ, Cho EJ: Gravinol ameliorates high-fructose-induced metabolic syndrome through regulation of lipid metabolism and proinflammatory state in rats. J Agric Food Chem 2008;56:5026–5032.
  9. Quesada H, del Bas JM, Pajuelo D, Diaz S, Fernandez-Larrea J, Pinent M, Arola L, Salvado MJ, Blade C: Grape seed proanthocyanidins correct dyslipidemia associated with a high-fat diet in rats and repress genes controlling lipogenesis and VLDL assembling in liver. Int J Obes (Lond) 2009;33:1007–1012.
  10. Lee YA, Cho EJ, Yokozawa T: Effects of proanthocyanidin preparations on hyperlipidemia and other biomarkers in mouse model of type 2 diabetes. J Agric Food Chem 2008;56:7781–7789.
  11. Iwasaki Y, Matsui T, Arakawa Y: The protective and hormonal effects of proanthocyanidin against gastric mucosal injury in Wistar rats. J Gastroenterol 2004;39:831–837.

    External Resources

  12. Del Bas JM, Ricketts ML, Baiges I, Quesada H, Ardevol A, Salvado MJ, Pujadas G, Blay M, Arola L, Blade C, Moore DD, Fernandez-Larrea J: Dietary procyanidins lower triglyceride levels signaling through the nuclear receptor small heterodimer partner. Mol Nutr Food Res 2008;52:1172–1181.
  13. Folch J, Lees M, Sloane Stanley GH: A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957;226:497–509.
  14. Ohnuki K, Haramizu S, Oki K, Watanabe T, Yazawa S, Fushiki T: Administration of capsiate, a non-pungent capsaicin analog, promotes energy metabolism and suppresses body fat accumulation in mice. Biosci Biotechnol Biochem 2001;65:2735–2740.
  15. Weir JB: New methods for calculating metabolic rate with special reference to protein metabolism. J Physiol 1949;109:1–9.
  16. Klaus S, Pultz S, Thone-Reineke C, Wolfram S: Epigallocatechin gallate attenuates diet-induced obesity in mice by decreasing energy absorption and increasing fat oxidation. Int J Obes (Lond) 2005;29:615–623.
  17. Murase T, Nagasawa A, Suzuki J, Hase T, Tokimitsu I: Beneficial effects of tea catechins on diet-induced obesity: stimulation of lipid catabolism in the liver. Int J Obes Relat Metab Disord 2002;26:1459–1464.
  18. Pataki T, Bak I, Kovacs P, Bagchi D, Das DK, Tosaki A: Grape seed proanthocyanidins improved cardiac recovery during reperfusion after ischemia in isolated rat hearts. Am J Clin Nutr 2002;75:894–899.
  19. Sano T, Oda E, Yamashita T, Naemura A, Ijiri Y, Yamakoshi J, Yamamoto J: Anti-thrombotic effect of proanthocyanidin, a purified ingredient of grape seed. Thromb Res 2005;115:115–121.
  20. Jang JK, Han JY: The antioxidant ability of grape seed extracts. Korean J Food Sci Technol 2002;34:524–528.
  21. Park SH, Park TS, Cha YS: Grape seed extract (Vitis vinifera) partially reverses high fat diet-induced obesity in C57BL/6J mice. Nutr Res Pract 2008;2:227–233.
  22. Furuyashiki T, Nagayasu H, Aoki Y, Bessho H, Hashimoto T, Kanazawa K, Ashida H: Tea catechin suppresses adipocyte differentiation accompanied by down-regulation of PPARγ2 and C/EBPα in 3T3-L1 cells. Biosci Biotechnol Biochem 2004;68:2353–2359.
  23. Hung PF, Wu BT, Chen HC, Chen YH, Chen CL, Wu MH, Liu HC, Lee MJ, Kao YH: Antimitogenic effect of green tea (–)-epigallocatechin gallate on 3T3-L1 preadipocytes depends on the ERK and Cdk2 pathways. Am J Physiol Cell Physiol 2005;288:C1094–C1108.
  24. Goncalves R, Mateus N, de Freitas V: Study of the interaction of pancreatic lipase with procyanidins by optical and enzymatic methods. J Agric Food Chem 2010;58:11901–11906.
  25. Moreno DA, Ilic N, Poulev A, Brasaemle DL, Fried SK, Raskin I: Inhibitory effects of grape seed extract on lipases. Nutrition 2003;19:876–879.
  26. Osumi T, Hashimoto T: Acyl-CoA oxidase of rat liver: a new enzyme for fatty acid oxidation. Biochem Biophys Res Commun 1978;83:479–485.
  27. Shepherd D, Yates DW, Garland PB: The rate-limiting step in the oxidation of palmitate or palmitoyl-coenzyme A by rat-liver mitochondria. Biochem J 1966;98:3C–4C.
  28. Trauner M, Arrese M, Wagner M: Fatty liver and lipotoxicity. Biochim Biophys Acta 2010;1801:299–310.
  29. Kawai N, Kawai T, Kawai K: Ultrasonic and laboratory studies on fatty liver in white-collar workers. Nippon Shokakibyo Gakkai Zasshi 1995;92:1058–1065.
  30. Maffei M, Halaas J, Ravussin E, Pratley RE, Lee GH, Zhang Y, Fei H, Kim S, Lallone R, Ranganathan S, et al: Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat Med 1995;1:1155–1161.
  31. Frederich RC, Hamann A, Anderson S, Lollmann B, Lowell BB, Flier JS: Leptin levels reflect body lipid content in mice: evidence for diet-induced resistance to leptin action. Nat Med 1995;1:1311–1314.

Pay-per-View Options
Direct payment This item at the regular price: USD 38.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50