Skin Pharmacol Physiol 2012;25:57–64

Water Extract of Gromwell (Lithospermum erythrorhizon) Enhances Migration of Human Keratinocytes and Dermal Fibroblasts with Increased Lipid Synthesis in an in vitro Wound Scratch Model

Kim H.a · Kim J.a · Park J.b · Kim S.H.c · Uchida Y.d · Holleran W.M.d · Cho Y.a
Departments of aMedical Nutrition and bEast-West Medical Science, Graduate School of East-West Medical Science, Kyung Hee University, Yongin-si, and cNutrex Co. Ltd., Seoul, Korea; dDermatology Service and Research Unit, Department of Veterans Affairs Medical Center, University of California, San Francisco, Calif., USA
email Corresponding Author

 goto top of outline Key Words

  • Gromwell
  • Migration
  • Lipid
  • Wound healing
  • Keratinocytes
  • Fibroblasts

 goto top of outline Abstract

Background: Although organic extracts of gromwell (Lithospermum erythrorhizon) have been shown to promote wound healing, the wound healing effects of water extracts of gromwell (WG) that are commonly used in traditional remedies have not been elucidated. Objective: We investigated whether WG promotes the migration and/or proliferation of cultured human keratinocytes (CHK) or dermal fibroblasts in parallel with increases in lipid synthesis during in vitro wound healing. Methods: CHK or fibroblasts were treated with 1–1,000 µg/ml WG for up to 48 h following scratch wound formation. Cell migration was assessed by measuring coverage (in percent) from the wound margin, while cell proliferation and lipid synthesis were determined by [3H]thymidine incorporation into DNA fractions, and [3H]palmitate or [3H]serine incorporation into lipid fractions, respectively. Results: Low-dose WG (1 µg/ml) enhanced the wound coverage for both CHK and fibroblasts at 24 h, while cell proliferation was not altered in either cell types. Synthesis of both total lipids and individual lipid classes, including phospholipids, sphingolipids and neutral lipids, were found to be increased at 24 h in CHK treated with 1 µg/ml WG; in similarly treated fibroblasts, only the syntheses of sphingolipids (such as ceramides and glucosylceramides), but not other lipid species, were significantly increased. In contrast, a higher dose of WG (10–1,000 µg/ml) did not enhance wound coverage, and 100 µg/ml WG neither altered cell proliferation nor lipid synthesis in both CHK and fibroblasts. Conclusion: Low-dose WG (1 µg/ml) enhances the migration of both CHK and fibroblasts with increased lipid synthesis in an in vitro wound scratch model.

Copyright © 2011 S. Karger AG, Basel

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 goto top of outline Author Contacts

Yunhi Cho
Department of Medical Nutrition, Graduate School of East-West Medical Science
Kyung Hee University
Yongin-si, Gyeonggi-do 446-701 (Korea)
Tel. +82 31 201 3817, E-Mail

 goto top of outline Article Information

H.K. and J.K. contributed equally to this study.

Received: November 29, 2010
Accepted after revision: June 14, 2011
Published online: October 4, 2011
Number of Print Pages : 8
Number of Figures : 2, Number of Tables : 3, Number of References : 32

 goto top of outline Publication Details

Skin Pharmacology and Physiology (Journal of Pharmacological and Biophysical Research)

Vol. 25, No. 2, Year 2012 (Cover Date: February 2012)

Journal Editor: Lademann J. (Berlin)
ISSN: 1660-5527 (Print), eISSN: 1660-5535 (Online)

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