For Manuscript Submission, Check or Review Login please go to Submission Websites List.
For the academic login, please select your country in the dropdown list. You will be redirected to verify your credentials.
Karyotype Differentiation between Two Stickleback Species (Gasterosteidae)Urton J.R.a, b · McCann S.R.a · Peichel C.L.a
aDivision of Human Biology, Fred Hutchinson Cancer Research Center, and bGraduate Program in Molecular and Cellular Biology, University of Washington, Seattle, Wash., USA Corresponding Author
Division of Human Biology, Fred Hutchinson Cancer Research Center
1100 Fairview Avenue North
Seattle, WA 98109-1024 (USA)
Tel. +1 206 667 1628, E-Mail email@example.com
The stickleback family (Gasterosteidae) of fish is less than 40 million years old, yet stickleback species have diverged in both diploid chromosome number (2n) and morphology. We used comparative fluorescence in situ hybridization (FISH) on 2 stickleback species, Gasterosteus aculeatus (2n = 42) and Apeltes quadracus (2n = 46), to ascertain the types of chromosome rearrangements that differentiate these species. The A. quadracus karyotype contains more acrocentric and telocentric chromosomes than the G. aculeatus karyotype. By using bacterial artificial chromosome probes from G. aculeatus in our FISH screen, we found that 6 pericentric inversions and 2 chromosome fusions/fissions are responsible for the greater number of acrocentric and telocentric chromosomes in A. quadracus. While most populations of G. aculeatus have an XX/XY sex chromosome system, A. quadracus has a ZZ/ZW sex chromosome system, as previously reported. However, we discovered that a population of A. quadracus from Connecticut lacks heteromorphic sex chromosomes, providing evidence for unexpected sex chromosome diversity in this species.
© 2011 S. Karger AG, Basel
Stickleback fishes are small teleosts of the family Gasterosteidae, which comprises 6 species that inhabit temperate freshwater and marine environments across the northern hemisphere [Wootton, 1976]. Previous studies on chromosome number and morphology revealed diverse karyotypes among stickleback species [Chen and Reisman, 1970; Ocalewicz et al., 2011] as well as within a single species [Kitano et al., 2009]. Stickleback species also have different sex chromosome systems. Both threespine sticklebacks (Gasterosteus aculeatus) and ninespine sticklebacks (Pungitius pungitius) have XX/XY (male heteromorphic) sex chromosome systems, but comparative fluorescence in situ hybridization (FISH) mapping and genetic linkage analyses indicate that these sex chromosome systems arose independently [Peichel et al., 2004; Ocalewicz et al., 2008; Ross and Peichel, 2008; Ross et al., 2009; Shapiro et al., 2009]. A third species, the fourspine stickleback (Apeltes quadracus), has a ZZ/ZW (female heteromorphic) sex chromosome system [Chen and Reisman, 1970; Ross et al., 2009]. In addition, the black-spotted stickleback (G. wheatlandi) and G. aculeatus populations from the Sea of Japan each have independently evolved X1X1X2X2/X1X2Y sex chromosome systems [Kitano et al., 2009; Ross et al., 2009].
The phylogenetic relationships between species in the stickleback family have previously been disputed [Mattern, 2004]. However, a recent comprehensive phylogeny has resolved the evolutionary relationship among the sticklebacks [Kawahara et al., 2009]. When all available karyotype data for sticklebacks are mapped onto this phylogeny, it appears that sticklebacks have diverged more rapidly in karyotype than previously appreciated, since even closely related stickleback species have different diploid chromosome numbers, karyotypes, and sex chromosome systems (fig. 1).
To explore the rapid evolution of stickleback karyotypes in more detail, we designed a comprehensive FISH survey to identify the major genomic rearrangements that have occurred in the karyotypes of 2 stickleback species since they diverged from a common ancestor. We chose 2 species with different diploid chromosome numbers and divergent karyotypes, the threespine stickleback (G. aculeatus) and the fourspine stickleback (A. quadracus) [Chen and Reisman, 1970; Ross et al., 2009]. In addition, while G. aculeatus has an XX/XY sex chromosome system found on chromosome 19 [Peichel et al., 2004; Ross and Peichel, 2008], the ancestral chromosome pair that evolved into the A. quadracus ZZ/ZW sex chromosome pair is not yet known. Thus, we designed our survey to uncover the genomic rearrangements that generated the diverse karyotypes of these species and to reveal the identity of the A. quadracus ZZ/ZW sex chromosome pair.
We collected male and female A. quadracus from the West River Memorial Park in New Haven, Connecticut, in May 2009. These males and females were brought into the laboratory and allowed to breed naturally in 110-liter aquarium tanks in July and August 2009. We grew progeny from these matings together in 474-liter aquarium tanks until adulthood. Tissue from both wild-caught A. quadracus individuals and their progeny was used to prepare metaphase chromosome spreads (see below). In April and June 2010, we collected male G. aculeatus from 2 locations in Lake Washington: Union Bay in Seattle, Washington and the Mercer Slough Nature Park in Bellevue, Washington (Washington permit 10-049). We housed these fish in 110-liter aquarium tanks. Metaphase chromosome spreads were prepared using tissue from wild-caught G. aculeatus individuals (see below). For both species, we kept fish in summer lighting conditions (16 h light, 8 h dark) at approximately 16°C in 0.35% saltwater (3.5 g/l Instant Ocean salt (Aquarium Systems, Mentor, Ohio, USA); 0.4 ml/l NaHCO3). We fed the fish with live brine shrimp nauplii twice daily. All procedures were approved by the Fred Hutchinson Cancer Research Center Institutional Animal Care and Use Committee (protocol 1575).
We used G. aculeatus bacterial artificial chromosome (BAC) clones from the CHORI-215 library [Kingsley et al., 2004] as probes for FISH (table 1). To identify BAC probes for each end of each G. aculeatus chromosome pair, we extracted 200 kb of genomic sequence from the end of each G. aculeatus chromosome assembly (BROAD S1 assembly, February 2006, available at http://www.ensembl.org/Gasterosteus_aculeatus/index.html) and used this sequence in a BLAST search of the publicly available BAC end sequences [Kingsley and Peichel, 2007]. If both the T7 and SP6 end sequence reads of the BAC clones aligned within the 200-kb genomic sequence we selected, we used those paired end sequence reads from that BAC in a BLAT search against the G. aculeatus genome. If the paired end sequence reads of the BAC clone aligned to the desired chromosome assembly in opposing orientation, with expected separation based on the average size of a CHORI-215 BAC insert [Kingsley et al., 2004], we then used the BAC clone as a FISH probe for that G. aculeatus chromosome (table 1).
For 18 of 21 G. aculeatus chromosomes, the selected BAC clones aligned to a region within 2.6 Mb of the predicted ends of that chromosome assembly and hybridized to the ends of chromosomes in G. aculeatus male metaphase spreads. For 3 chromosomes, 1 BAC clone aligned to a genomic sequence within 2.6 Mb of the predicted ends of that chromosome assembly, but did not hybridize to the end of a chromosome pair in G. aculeatus male metaphase spreads. In those 3 cases, we screened additional 200-kb regions of those chromosome assemblies until we identified BAC clones that met the above selection criteria and hybridized to the ends of a chromosome pair in male G. aculeatus metaphase spreads. For the chromosomes involved (chromosomes 16, 19 and 20), errors in the G. aculeatus genome assembly, as previously reported for chromosome 19 [Ross and Peichel, 2008], or population-specific chromosome rearrangements could account for these observations.
We prepared metaphase spreads as described previously [Ross and Peichel, 2008] with the following modifications. We performed intraperitoneal injections of 5 µl (A. quadracus) or 10 µl (G. aculeatus) of 1% colchicine in phosphate-buffered saline into adult fish and incubated them for 12–14 h in an aquarium. After euthanizing the fish in 0.025% tricaine methanesulfonate (MS-222), we determined the sex of individual fish by examination of the gonads. We combined spleen tissue from several individuals to make metaphase spreads of males and females from both species (table 2).
We conducted FISH as previously described [Ross and Peichel, 2008], with the following modifications. We extracted and purified BAC DNA using an AutoGen 740 automated system (AutoGen, Holliston, Mass., USA) and quantified BAC DNA by gel electrophoresis. Using the Vysis nick translation kit (Abbott Labs, North Chicago, Ill., USA), we labeled 1 µg of each BAC clone with either ChromaTide Alexa Fluor 488-5-dUTP or 568-5-dUTP (Invitrogen, Carlsbad, Calif., USA). Two hundred nanograms of the labeled clones were ethanol precipitated with 10 µg salmon sperm DNA and 10 µg genomic DNA from the species used in the experiment. We allowed hybridization to proceed for 1–2 nights at 37°C. We viewed washed slides using the 100× objective on a Nikon Eclipse 80i microscope (Nikon, Shinjuku, Tokyo, Japan) with an automated filter turret using Chroma filters 31000v2 (DAPI), 41001 (FITC), and 41004 (Texas Red) (Chroma, Bellows Falls, Vt., USA). We captured images using a Photometrics Coolsnap ES2 camera (Photometrics, Tucson, Ariz., USA) and used NIS Elements imaging software (BR 3.00, SP7, Hotfix8, Build 548, Nikon, Shinjuku, Tokyo, Japan) to pseudocolor those images grey (DAPI), green (Alexa 488) and purple (Alexa 568).
For G. aculeatus and A. quadracus, we used images from FISH experiments (figs. 2, 3; data not shown) to assess the morphology of each chromosome. We classified chromosomes according to this average arm length ratio (long arm:short arm, L:S). Chromosomes were classified as metacentric (1.0–1.7), submetacentric (1.7–3.0), acrocentric (3.0–7.0), or telocentric (7.0 or greater), following Levan et al.  and Schrader et al.  (table 3). The only exception to this method is G. aculeatus chromosome 19, which is a heteromorphic XX/XY sex chromosome pair [Peichel et al., 2004; Ross and Peichel, 2008]. Thus, we measured the arm lengths of the single X chromosome in male G. aculeatus, but did not measure the Y chromosome (fig. 3; data not shown). For all FISH experiments and karyotype analyses, we examined multiple metaphase spreads from multiple pools of individual fish (table 2). Based on the chromosome morphology, relative centromere position, and BAC probe hybridization positions for each chromosome in our FISH screen, we determined the types of chromosome rearrangements that could explain the observed differences between the G. aculeatus and A. quadracus karyotypes (table 3).
We also calculated the fundamental number (major chromosome arm number; NF) for both sexes of G. aculeatus and A. quadracus [Matthey, 1949; Klinkhardt, 1998]. For calculating NF, we followed White  and Klinkhardt  and considered that metacentric and submetacentric chromosomes each have 2 major chromosome arms, while acrocentric and telocentric chromosomes each have 1 major chromosome arm. We inferred NF and other karyotype features for G. aculeatus females (table 4) based on the absence of the metacentric Y and the presence of a second submetacentric X chromosome [Ross and Peichel, 2008].
The G. aculeatus male karyotype consists of 42 chromosomes: 8 autosomes are metacentric, 6 are submetacentric, 16 are acrocentric, 10 are telocentric, and there is 1 submetacentric X and 1 metacentric Y (table 4) [Ross and Peichel, 2008]. The relatively high NF (58) for G. aculeatus compared to its diploid number (2n = 42) reflects a relatively large number of metacentric and submetacentric chromosomes in the karyotype. The selected BAC clones (table 1) hybridize by FISH to the ends of each chromosome (figs. 2, 3). By combining FISH with measurements of chromosome arm length, we were able to assign each G. aculeatus chromosome pair to a morphological class (table 3).
We used the same BAC clones selected for the G. aculeatus male FISH analysis in a similar survey of the chromosomes in A. quadracus females and males (figs. 2, 3). The A. quadracus karyotype consists of 46 chromosomes, of which 6 chromosomes are metacentric, 4 are submetacentric, 32 are acrocentric, and 4 are telocentric (fig. 4; table 4). The dominance of acrocentric and telocentric chromosomes in the A. quadracus karyotype (78%) is reflected in a lower ratio of NF to diploid chromosome number; NF:2n is 56:46 for A. quadracus, while it is 58:42 for G. aculeatus (table 4).
Two of the larger chromosome pairs in the G. aculeatus karyotype, chromosomes 4 (submetacentric) and 7 (metacentric), each correspond to 2 pairs of relatively small acrocentric chromosomes in A. quadracus (fig. 2; table 3). Eight chromosome pairs appear to have undergone inversions in the time since G. aculeatus and A. quadracus diverged; 6 are pericentric inversions, which encompass the centromere, and 2 are paracentric inversions, which do not encompass the centromere (fig. 2; table 3). Five of these pericentric inversions have resulted in visibly different morphology for the orthologous G. aculeatus and A. quadracus chromosomes. Probes from 11 G. aculeatus chromosome pairs show identical hybridization patterns and morphologies in both G. aculeatus and A. quadracus (fig. 3).
A heteromorphic ZZ/ZW sex chromosome pair has been described in A. quadracus populations from Maine [Chen and Reisman, 1970] and Massachusetts [Ross et al., 2009]. However, we found no evidence for a heteromorphic sex chromosome pair in A. quadracus male or female progeny from specimens collected in the West River in New Haven, Connecticut (fig. 4). In addition, hybridization patterns were identical in metaphase spreads from male and female A. quadracus for all FISH probes used in this study (data not shown).
We undertook a comparative FISH study to identify the major genomic rearrangements that have occurred in lineages separating 2 stickleback species, A. quadracus and G. aculeatus. These species are intriguing subjects for such a study, not only because of reported differences in karyotype, but also due to the divergence of sex chromosome systems [Chen and Reisman, 1970; Ross et al., 2009]. In G. aculeatus, chromosomes 19 are an XX/XY sex chromosome pair [Peichel et al., 2004; Ross and Peichel, 2008]. Two previous studies have shown that A. quadracus populations from Maine and Massachusetts have a ZZ/ZW sex chromosome pair [Chen and Reisman, 1970; Ross et al., 2009]. In both studies, the W chromosome is described as a large acrocentric chromosome, while the Z chromosome is also acrocentric, but has a smaller long arm than the W chromosome. Genetic markers from chromosome 19 are not linked to the sex determination locus in an A. quadracus family from Massachusetts [Ross et al., 2009], and no genetic markers linked to a sex determination locus have been found in A. quadracus.
In the present study of A. quadracus from Connecticut, we find that neither females nor males have a heteromorphic chromosome pair indicative of either a ZZ/ZW or XX/XY sex chromosome system. Two possible explanations could account for the differences in sex chromosomes between the Connecticut population of A. quadracus and the Maine and Massachusetts populations. First, A. quadracus from Connecticut could have a cryptic (homomorphic) ZZ/ZW sex chromosome pair that is homologous to the ZZ/ZW pair in the Massachusetts and Maine populations. The larger size of the W chromosome relative to the Z chromosome in those populations [Chen and Reisman, 1970; Ross et al., 2009] could be due to an increase in the amount of heterochromatin in the W chromosome relative to the Z chromosome following the cessation of recombination with the Z chromosome. If negligible amounts of heterochromatin were found in the A. quadracus W chromosome of the Connecticut population, then the Z and W chromosomes in this population would not be morphologically distinguishable. Such a population-specific difference in the amount of heterochromatin or repetitive DNA sequences present on the sex chromosomes has been reported in the guppy Poecilia reticulata [Hornaday et al., 1994], the platyfish Xiphophorus maculatus [Nanda et al., 2000], and the glass knifefish Eigenmannia virescens [Almeida-Toledo et al., 2001]. Differences in the amount of heterochromatin on the sex chromosomes have also been reported between closely related species of salmonids [Moran et al., 1996] and South American catfishes [Andreata et al., 1992].
Second, A. quadracus from Connecticut could have a different sex determination mechanism than the Massachusetts and Maine ZZ/ZW sex chromosome system. This mechanism could be genetic, environmental, or a combination of genetic and environmental signals. Extreme variation in the mechanism of sex determination within a single species is not without precedent. In the Atlantic silverside, Menidia menidia, both environmental and genetic sex determination mechanisms are present and vary by latitude [Conover and Heins, 1987]. Although we currently have little data on the ecology or population structure of A. quadracus, it will be interesting to determine whether variation in sex determination mechanisms is associated with any ecological or geographical factors in this species.
Even though the A. quadracus population in this study does not have a heteromorphic ZZ/ZW sex chromosome system, our comparative FISH study could help to identify the ZZ/ZW sex chromosome system in A. quadracus. The ZZ/ZW pair in both Massachusetts and Maine populations of A. quadracus is one of the larger chromosome pairs in the karyotype [Chen and Reisman, 1970; Ross et al., 2009]. In addition, the karyotypes of the Connecticut and Massachusetts A. quadracus are similar, differing only in the presence of the large W chromosome in A. quadracus females from the Massachusetts population [Ross et al., 2009]. Thus, the heteromorphic ZZ/ZW pair in the Massachusetts A. quadracus karyotype could correspond to one of the larger chromosome pairs in the Connecticut A. quadracus karyotype. Our FISH experiments showed that chromosomes 1, 2, 9, 12, 13, 19, and 20 were among the larger chromosome pairs in their respective metaphase spreads. Two of these (chromosomes 12 and 19) have previously been ruled out as the Massachusetts A. quadracus ZZ/ZW pair [Ross et al., 2009]; however, the other large chromosomes remain targets for further studies to identify the ZZ/ZW sex chromosome pair in A. quadracus.
Population differences in sex chromosomes and sex determination mechanisms are also found in other species of fish. Studies have documented sex chromosome diversity within species of poeciliid fish [Nanda et al., 2000; Volff and Schartl, 2001; Schultheis et al., 2009] and within several species of neotropical fish, including E. virescens [Almeida-Toledo et al., 2002; Henning et al., 2011], Erythrinus erythrinus [Bertollo et al., 2004; Cioffi et al., 2010], and Hoplias malabaricus [Bertollo et al., 2000; Cioffi and Bertollo, 2010]. Sex chromosome systems also differ among populations of other stickleback species. G. aculeatus from the Sea of Japan have an X1X1X2X2/X1X2Y sex chromosome system, which likely arose from the ancestral XX/XY sex chromosome system found in other G. aculeatus populations [Kitano et al., 2009]. Black-spotted sticklebacks (G. wheatlandi) from a population in Maine have an XX/XY sex chromosome system [Chen and Reisman, 1970], while at least 1 G. wheatlandi population in Massachusetts has an X1X1X2X2/X1X2Y sex chromosome system [Ross et al., 2009]. Future studies should reveal whether most A. quadracus populations have ZZ/ZW sex chromosomes, or if this species has a diverse array of sex determination mechanisms.
Although G. aculeatus and A. quadracus diverged approximately 40 million years ago, our comparative FISH study has uncovered genomic rearrangements encompassing nearly half of the 21 G. aculeatus chromosome pairs. These rearrangements account for the 2 major karyotypic differences between these species: A. quadracus has a higher diploid number, coupled with a lower number of metacentric and submetacentric chromosomes, when compared to G. aculeatus (table 4).
The larger diploid number (46) in A. quadracus is explained by our finding that 2 G. aculeatus chromosome pairs (4 and 7) correspond to 4 A. quadracus chromosome pairs. Examination of other stickleback species does not reveal whether the G. aculeatus or A. quadracus diploid number is more similar to the ancestral state (fig. 1). The closest extant relative of G. aculeatus also has a diploid chromosome number of 42, as does a more distant relative, the ninespine stickleback, P. pungitius [Ocalewicz et al., 2008, 2011; Ross et al., 2009; Shapiro et al., 2009]. However, many differences in chromosome morphology exist between the Gasterosteus species and P. pungitius [Ocalewicz et al., 2008, 2011; Ross et al., 2009]. A recent report shows that P. pungitius has a higher NF:2n than G. aculeatus [Ocalewicz et al., 2011], indicating a higher number of metacentric and submetacentric chromosome pairs in P. pungitius. However, it is not known if the chromosome pairs that differ in morphology in G. aculeatus and P. pungitius are identical to the chromosome pairs that differ in G. aculeatus and A. quadracus in this study. The closest extant relative of P. pungitius, the brook stickleback (Culaea inconstans), has the same diploid number as A. quadracus, but these 2 species also differ in karyotype [Chen and Reisman, 1970; Ross et al., 2009]. No published reports document the diploid chromosome number and chromosome morphology of the fifteenspine stickleback (Spinachia spinachia), which is the closest extant relative of A. quadracus [Kawahara et al., 2009]. Thus, we lack comprehensive karyotype data from a sufficient number of stickleback species and populations to know whether the karyotype of G. aculeatus or A. quadracus is the more ancestral for this family.
Though the ancestral stickleback karyotype is unknown, our FISH screen has clarified some of the mechanisms that have shaped the A. quadracus and G. aculeatus karyotypes since they diverged. While metacentric and submetacentric chromosomes make up less than one quarter of the A. quadracus karyotype, they comprise more than one third of the G. aculeatus karyotype (table 4). One factor that contributes to this difference is the separation of G. aculeatus chromosomes 4 and 7 in the A. quadracus karyotype (fig. 2; table 3). The differential state of these chromosomes in G. aculeatus and A. quadracus, and the fact that they are both acrocentric in A. quadracus, suggest that Robertsonian fusions and/or centric fissions have played roles in the evolution of stickleback karyotypes. These fusions may leave signatures in the form of interstitial telomeric sequences (ITSs) in the species with the derived chromosome state. A recent study of a G. aculeatus population from Poland failed to detect any ITSs by FISH; however, it is possible that ITSs were eliminated or remain in a copy number that is below the detection threshold for FISH [Ocalewicz et al., 2011]. Thus, the ancestral state of chromosomes 4 and 7 in the stickleback family is still unknown, and proper determination of the ancestral state will clarify whether fusions or fissions are responsible for these differences in karyotype.
We also uncovered evidence for inversions involving at least 8 chromosomes, based on differences in relative centromere position and/or BAC probe hybridization positions between G. aculeatus and A. quadracus. Six of these inversion events encompass the centromere, and 5 of those inversions alter the gross chromosome morphology between G. aculeatus and A. quadracus. In particular, 3 of these 5 inversions are on chromosomes that are metacentric or submetacentric in G. aculeatus and acrocentric or telocentric in A. quadracus (table 3), which is one factor that accounts for the lower NF in this species compared to G. aculeatus (table 4). Future studies of chromosome morphology and relative probe hybridization positions in other stickleback species could further illuminate the role of pericentric inversions in the evolution of karyotypes across this family.
Our FISH survey has identified the major chromosome rearrangements that have shaped the G. aculeatus and A. quadracus karyotypes since they diverged approximately 35 million years ago. The A. quadracus karyotype contains a large number of acrocentric and telocentric chromosomes that can be explained by several pericentric inversions and Robertsonian fusion or centric fission events. Importantly, we discovered unexpected diversity among A. quadracus sex chromosomes. Male and female individuals from Connecticut do not harbor heteromorphic (distinguishable) sex chromosomes, in contrast to other A. quadracus populations in New England. Future studies are required to uncover the true diversity of sex chromosomes and sex determination mechanisms in this species and to learn how the diverse array of sex chromosome systems evolved in the stickleback family.
We thank Thomas Near for providing our wild-caught A. quadracus samples, the Broad Institute and Stanford Human Genome Center for allowing us to use BAC end sequences and the initial stickleback genome sequence assembly prior to publication, Joseph Ross for providing both protocols and training for stickleback cytogenetics and FISH, Kohta Yoshida for helpful discussions on chromosome evolution in fishes, and Anna Greenwood for her unique insights, discussions, and advice as this project progressed. We thank Barbara Trask, Joseph Ross, and Anna Greenwood for helpful comments and suggestions regarding this manuscript. Finally, we thank Stephen Uy, who translated key passages from Robert Matthey’s Les Chromosomes des Vertébrés. Our work was funded by National Institutes of Health grants P50 HG002568 (C.L.P.), R01 GM089733 (C.L.P.), and T32 HG00035 (J.R.U.).
Division of Human Biology, Fred Hutchinson Cancer Research Center
1100 Fairview Avenue North
Seattle, WA 98109-1024 (USA)
Tel. +1 206 667 1628, E-Mail firstname.lastname@example.org
Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.