Journal Mobile Options
Table of Contents
Vol. 45, No. 6, 2011
Issue release date: December 2011
Caries Res 2011;45:574–580
(DOI:10.1159/000334623)

A Randomised Trial Comparing the Antibacterial Effects of Dentine Primers against Bacteria in Natural Root Caries

Rolland S.L. · McCabe J.F. · Imazato S. · Walls A.W.G.
aSchool of Dental Sciences, Newcastle University, Newcastle upon Tyne, UK; bGraduate School of Dentistry, Osaka University, Suita, Japan

Individual Users: Register with Karger Login Information

Please create your User ID & Password





Contact Information











I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Abstract

As people are living longer and retaining their teeth into old age, root caries is an increasingly significant problem. A minimally invasive treatment strategy, involving sealing the root caries lesion with an antibacterial resin sealant, could be highly beneficial. The aim of this study was to compare the antibacterial properties of the primers of two proprietary dentine bonding agents, Clearfil SE Bond (SE; Kuraray Medical, Japan) and Clearfil Protect Bond (PB; Kuraray Medical), which contains the antibacterial monomer methacryloyloxydodecylpyridinium bromide. Fifty-two root caries lesions were identified and randomly assigned to a primer. The lesion was cleaned, isolated, sampled with a sharp spoon excavator, a primer applied and a second sample taken. Samples were transported in fastidious anaerobe broth, vortex-dispersed and serial dilutions inoculated onto selective agars. Reduction in colony-forming units (CFU, %) after primer application was calculated for both primers for bacterial growth on each selective agar and compared to a hypothesised mean of 100% (one-sample t test, p < 0.05). No significant differences between primers were seen, indicating efficient bacterial elimination by both materials. Comparing percent reduction between SE and PB for each agar (Mann-Whitney test, p < 0.05), a significantly greater CFU reduction by PB was seen for streptococci but not other bacteria. More lesions exhibited bacterial growth and several lesions demonstrated marked bacterial growth after treatment with SE compared with PB. Therefore, PB appears to exhibit superior antimicrobial properties, particularly against streptococci. Both primers are highly antibacterial towards root caries bacteria and may therefore be suitable for minimally invasive treatment.



Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

References

  1. Bapna MS, Murphy R, Mukherjee S: Inhibition of bacterial colonization by antimicrobial agents incorporated into dental resins. J Oral Rehabil 1988;15:405–411.
  2. Beighton D, Lynch E: Comparison of selected microflora of plaque and underlying carious dentine associated with primary root caries lesions. Caries Res 1995;29:154–158.
  3. Beighton D, Lynch E, Heath MR: A microbiological study of primary root-caries lesions with different treatment needs. J Dent Res 1993;72:623–629.
  4. Cehreli ZC, Stephan A, Sener B: Antimicrobial properties of self-etching primer-bonding systems. Oper Dent 2003;28:143–148.
  5. Cooper I, Kelly M, Walker A, Britain G: Adult Dental Health Survey: Oral Health in the United Kingdom 1998: A Survey. London, Stationery Office, 2000.
  6. Doi J, Itota T, Yoshiyama M, Tay FR, Pashley DH: Bonding to root caries by a self-etching adhesive system containing MDPB. Am J Dent 2004;17:89–93.
  7. Felton D, Bergenholtz G, Cox CF: Inhibition of bacterial growth under composite restorations following GLUMA pretreatment. J Dent Res 1989;68:491–495.
  8. Feuerstein O, Matalon S, Slutzky H, Weiss EI: Antibacterial properties of self-etching dental adhesive systems. J Am Dent Assoc 2007;138:349–354.
  9. Hahn P, Schaller HG, Gernhardt C, Hellwig E: Influence of two dentin bonding systems on the demineralization of the root surface. Oper Dent 1999;24:344–350.
  10. Imazato S: Antibacterial properties of resin composites and dentin bonding systems. Dent Mater 2003;19:449–457.
  11. Imazato S, Ebi N, Tarumi H, Russell RR, Kaneko T, Ebisu S: Bactericidal activity and cytotoxicity of antibacterial monomer MDPB. Biomaterials 1999;20:899–903.
  12. Imazato S, Imai T, Ebisu S: Antibacterial activity of proprietary self-etching primers. Am J Dent 1998a;11:106–108.
  13. Imazato S, Imai T, Russell RR, Torii M, Ebisu S: Antibacterial activity of cured dental resin incorporating the antibacterial monomer MDPB and an adhesion-promoting monomer. J Biomed Mater Res 1998b;39:511–515.
  14. Imazato S, Kaneko T, Takahashi Y, Noiri Y, Ebisu S: In vivo antibacterial effects of dentin primer incorporating MDPB. Oper Dent 2004;29:369–375.
  15. Imazato S, Kinomoto Y, Tarumi H, Ebisu S, Tay FR: Antibacterial activity and bonding characteristics of an adhesive resin containing antibacterial monomer MDPB. Dent Mater 2003;19:313–319.
  16. Imazato S, Kinomoto Y, Tarumi H, Torii M, Russell RR, McCabe JF: Incorporation of antibacterial monomer MDPB into dentin primer. J Dent Res 1997;76:768–772.
  17. Imazato S, Kuramoto A, Kaneko T, Ebisu S, Russell RR: Comparison of antibacterial activity of simplified adhesive systems. Am J Dent 2002a;15:356–360.
  18. Imazato S, Kuramoto A, Takahashi Y, Ebisu S, Peters MC: In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond. Dent Mater 2006;22:527–532.
  19. Imazato S, Tay FR, Kaneshiro AV, Takahashi Y, Ebisu S: An in vivo evaluation of bonding ability of comprehensive antibacterial adhesive system incorporating MDPB. Dent Mater 2007;23:170–176.
  20. Imazato S, Torii Y, Takatsuka T, Inoue K, Ebi N, Ebisu S: Bactericidal effect of dentin primer containing antibacterial monomer methacryloyloxydodecylpyridinium bromide (MDPB) against bacteria in human carious dentin. J Oral Rehabil 2001;28:314–319.
  21. Imazato S, Walls AW, Kuramoto A, Ebisu S: Penetration of an antibacterial dentine-bonding system into demineralized human root dentine in vitro. Eur J Oral Sci 2002b;110:168–174.
  22. Kuramoto A, Imazato S, Walls AW, Ebisu S: Inhibition of root caries progression by an antibacterial adhesive. J Dent Res 2005;84:89–93.
  23. Lobo MM, Goncalves RB, Pimenta LA, Bedran-Russo AK, Pereira PN: In vitro evaluation of caries inhibition promoted by self-etching adhesive systems containing antibacterial agents. J Biomed Mater Res B Appl Biomater 2005;75:122–127.

    External Resources

  24. Meiers JC, Miller GA: Antibacterial activity of dentin bonding systems, resin-modified glass ionomers, and polyacid-modified composite resins. Oper Dent 1996;21:257–264.
  25. Ohmori K, Maeda N, Kohno A: Evaluation of antibacterial activity of three dentin primers using an in vitro tooth model. Oper Dent 1999;24:279–285.
  26. Ozer F, Karakaya S, Unlu N, Erganis O, Kav K, Imazato S: Comparison of antibacterial activity of two dentin bonding systems using agar well technique and tooth cavity model. J Dent 2003;31:111–116.
  27. Ozer F, Unlu N, Karakaya S, Ergani O, Hadimli HH: Antibacterial activities of MDPB and fluoride in dentin bonding agents. Eur J Prosthodont Restor Dent 2005;13:139–142.
  28. Polydorou O, Pelz K, Hahn P: Antibacterial effect of an ozone device and its comparison with two dentin-bonding systems. Eur J Oral Sci 2006;114:349–353.
  29. Schmalz G, Ergucu Z, Hiller KA: Effect of dentin on the antibacterial activity of dentin bonding agents. J Endod 2004;30:352–358.
  30. Sehgal V, Shetty VS, Mogra S, Bhat G, Eipe M, Jacob S, Prabu L: Evaluation of antimicrobial and physical properties of orthodontic composite resin modified by addition of antimicrobial agents: an in-vitro study. Am J Orthod Dentofacial Orthop 2007;131:525–529.
  31. Steele JG, Sheiham A, Marcenes W, Walls AW: National Diet and Nutrition Survey: People Aged 65 Years and Over. London, Stationery Office, 1998.
  32. Takahashi Y, Imazato S, Russell RR, Noiri Y, Ebisu S: Influence of resin monomers on growth of oral streptococci. J Dent Res 2004;83:302–306.
  33. Turkun LS, Ates M, Turkun M, Uzer E: Antibacterial activity of two adhesive systems using various microbiological methods. J Adhes Dent 2005;7:315–320.
  34. Turkun M, Turkun LS, Ergucu Z, Ates M: Is an antibacterial adhesive system more effective than cavity disinfectants? Am J Dent 2006;19:166–170.
  35. Tziafas D, Koliniotou-Koumpia E, Tziafa C, Papadimitriou S: Effects of a new antibacterial adhesive on the repair capacity of the pulp-dentine complex in infected teeth. Int Endod J 2007;40:58–66.


Pay-per-View Options
Direct payment This item at the regular price: USD 38.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50