Table of Contents
To view the fulltext, log-in or choose pay-per-view options:
Get Access

Molecular Diagnosis and Species Identification of Mucosal Leishmaniasis in Iran and Correlation with Cytological Findings

Shirian S.a, d · Oryan A.a · Hatam G.-R.b · Daneshbod K.c · Daneshbod Y.c
aDepartment of Pathobiology, School of Veterinary Medicine, Shiraz University, bCenter of Basic Researches in Infectious Diseases, School of Medicine, Shiraz University of Medical Sciences, and cDepartment of Cytopathology, Dr. Daneshbod Pathology Laboratory, Shiraz, Iran; dInstitute of Experimental Pathology, Münster, Germany Acta Cytologica 2012;56:304–309 (DOI:10.1159/000337450)

Abstract

Objective: Mucosal leishmaniasis (ML) is a rare destructive disease that mainly affects the mucous membranes of the mouth and nose. The etiologic agent(s) of ML are not well known in the Middle East. Study Design: Cytologic smears of ML from the mucosal lesions of 7 patients were prepared by scraping. In 2 patients with nasal lesions, exfoliative cytology was made by washing the nasal cavity. The smears were both air dried and fixed in alcohol and stained. Scrapings from the same smears were then tested for leishmanial DNA by nested PCR. Results: This study characterized 9 isolates of ML, with 7 cases identified as Leishmania major and 2 as Leishmania tropica. While 6 patients were found to be positive by the cytology technique, the nested PCR was positive for all of these samples. Conclusions: Presence of granuloma and multinucleated giant cells in the negative smears of the patients who showed clinical manifestation of ML was an important clue for diagnosis of this disease. The PCR-based method not only appears to be a precise diagnostic approach in the identification of suspected cases of ML but is also efficient in determining the species of the parasite. L. major and L. tropica can lead to ML, but they result in different cytologic features.

 

Individual Users: Register with Karger Login Information

Please create your User ID & Password





Contact Information











I have read the Karger Terms and Conditions and agree.


Pay-per-View Options
Direct payment This item at the regular price: USD 38.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50