Mitochondrial Factors and VACTERL Association-Related Congenital MalformationsSiebel S. · Solomon B.D.
Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Md., USA Corresponding Author
VACTERL/VATER association is a group of congenital malformations characterized by at least 3 of the following findings: vertebral defects, anal atresia, cardiac defects, tracheo-esophageal fistula, renal anomalies, and limb abnormalities. To date, no unifying etiology for VACTERL/VATER association has been established, and there is strong evidence for causal heterogeneity. VACTERL/VATER association has many overlapping characteristics with other congenital disorders that involve multiple malformations. In addition to these other conditions, some of which have known molecular causes, certain aspects of VACTERL/VATER association have similarities with the manifestations of disorders caused by mitochondrial dysfunction. Mitochondrial dysfunction can result from a number of distinct causes and can clinically manifest in diverse presentations; accurate diagnosis can be challenging. Case reports of individuals with VACTERL association and confirmed mitochondrial dysfunction allude to the possibility of mitochondrial involvement in the pathogenesis of VACTERL/VATER association. Further, there is biological plausibility involving mitochondrial dysfunction as a possible etiology related to a diverse group of congenital malformations, including those seen in at least a subset of individuals with VACTERL association.
© 2013 S. Karger AG, Basel
Friedrich Nietzsche’s observation that ‘all that is profound loves the mask’ holds true for many aspects of medical science, especially for certain relatively esoteric medical conditions. In this review, we hope to address potential connections between 2 disorders: VACTERL association and mitochondropathies. These disorders may be thought of as profound and ‘masked’ in terms of clinical and biological challenges related to understanding, diagnosing and managing these conditions. In order to describe this association and to allow this review article to be accessible and informative to a wide variety of clinicians and researchers, we will discuss the following topics: definitions of VATER/ VACTERL association; clinical and biological aspects of mitochondria in health and disease; case reports implicating mitochondrial dysfunction in VATER/ VACTERL association; potential extrapolations and links to other disorders.
VATER association was first defined in the early 1970s by Quan and Smith [1972, 1973] to describe patients found to have a nonrandom association of congenital anomalies: vertebral defects, anal atresia, tracheo-esophageal fistula with esophageal atresia, radial and renal dysplasia. The acronym was later expanded to VACTERL to account for patients with additional congenital anomalies including cardiovascular and limb defects other than radial dysplasia [Quan and Smith, 1972, 1973; Temtamy and Miller, 1974; Nora and Nora, 1975].
VACTERL association has an estimated frequency of approximately 1–9/100,000 infants [Khoury et al., 1983; Czeizel and Ludányi, 1985; Botto et al., 1997] and has no known ethnic or population-based predilection [Khoury et al., 1983; Czeizel and Ludányi , 1985; Weaver et al., 1986; Evans et al., 1992, 1999; Rittler et al., 1996; Botto et al., 1997; Källén et al., 2001; Seo et al., 2010; Solomon et al., 2010b, c; Wijers et al., 2010]. The estimated incidence is challenging due to variable diagnostic criteria, clinical heterogeneity [reviewed in Solomon, 2011] and the overlap with many other conditions [Evans et al., 1992, 1999; Rittler et al., 1996; Källén et al., 2001; Solomon et al., 2010b, c].
To date, no unifying or major etiology has been identified, and there is strong evidence for causal heterogeneity [Khoury et al., 1983; Evans et al., 1992, 1999; Solomon et al., 2010b, c]. Possible candidate genes have been reported in isolated reports, as have other factors, such as primary and secondary mitochondrial dysfunction and exposure to known teratogens, including gestational diabetes, maternal smoking, alcohol, and lead exposure in pregnancy [Nora et al., 1978; Czeizel and Ludányi, 1985; Levine and Muenke, 1991; Tüzel et al., 2007; Zhao et al., 2007; Castori et al., 2008; van Rooij et al., 2010; Wijers et al., 2010].
Mitochondrial dysfunction is an emerging area of interest as a plausible etiology of VACTERL association in at least a small proportion of affected individuals [Chan et al., 2002; Ornoy, 2007; Ornoy et al., 2010; Wang and Moley, 2010]. To date, 6 individuals with VACTERL association and demonstrated mitochondrial dysfunction have been described [Cormier-Daire et al., 1996; Damian et al., 1996; von Kleist-Retzow et al., 2003; Thauvin-Robinet et al., 2006; Solomon et al., 2011].
Basic Mitochondrial Biology
Mitochondria are ubiquitous, essential organelles of energy metabolism in nucleated mammalian cells. The number of mitochondria in a cell varies widely by organism and tissue type [Alberts et al., 1994; Voet et al., 2006]. A mitochondrion is composed of different compartments with individual functions (fig. 1). The inner mitochondrial membrane houses the oxidative phosphorylation (OXPHOS) system, which generates ATP, the major source of cellular energy. The mitochondrial matrix, enclosed by the inner membrane, contains enzymes involved in amino acid, carbohydrate, and lipid metabolism, mitochondrial ribosomes, and the mitochondrial genome [Alberts et al., 1994], the latter of which encodes key enzymes involved in energy metabolism. In addition to energy production, mitochondria play a central role in membrane potential regulation [Voet et al., 2006], apoptosis-programmed cell death [Green, 1998], calcium storage and signaling [Hajnóczky et al., 2006], and regulation of oxidative stress.
|Fig. 1. Overview of mitochondrial location and anatomy, showing a human cell containing multiple mitochondria, as well as the respiratory chain complexes. Many mitochondrial proteins are encoded by nuclear DNA and transported into the mitochondria by specialized transport systems (arrow). Multiple mtDNA molecules are located within the mitochondrial matrix. Each mtDNA molecule encodes 37 proteins, of which 13 are part of the respiratory chain. The respiratory chain, shown in more detail in the bottom portion of the figure, is located on the inner mitochondrial membrane, and consists of 5 different complexes. Within the mitochondrial matrix, pyruvate enters the tricyclic acid cycle, which is linked with the respiratory chain through complex II. Except for complex II, all complexes of the respiratory chain are encoded by both nuclear and mtDNA.|
Of special interest in the context of VACTERL association is the OXPHOS system and the citric acid cycle (Krebs) cycle. The OXPHOS system refers to a multienzyme complex on the inner mitochondrial membrane consisting of NADH Coenzyme Q-reductase (complex I), succinate CoQ reductase (complex II), CoQ-H2 cytochrome reductase (complex IV), and ATP-synthase (complex V). Complexes I–IV constitute the respiratory chain system. The citric acid cycle cycle is located in the matrix; the initial rate limiting step is controlled via the multienzyme pyruvate dehydrogenase complex. There is evidence that citric acid cycle and/or pyruvate metabolism defects are causally involved in congenital anomalies (see below) [von Kleist-Retzow et al., 2003].
Mitochondria have their own unique genome; mitochondrial proteins may be encoded by nuclear or mitochondrial DNA (mtDNA) such that a mutation in either genome can lead to aberrant mitochondrial enzyme activity. Over 250 different mtDNA mutations have been reported in humans, and these mutations have been identified as disease-causing in approximately 1 in 5,000 overall individuals, with an incidence of pathogenic variants reported in 1 in 200 live births (however, it is important to note that the presence of a pathogenic variant will not necessarily lead to clinical manifestations) [Chinnery et al., 2000; Skladal et al., 2003; Elliott et al., 2008; Schaefer et al., 2008; Spinazzola, 2011].
Mammalian cells contain a varying but large number of mitochondria. As all mitochondria in the zygote are derived from the oocyte (with rare exceptions reported), mtDNA mutations can only be transmitted from a female carrier to her offspring [Schwartz and Vissing, 2002]. Conversely, conditions resulting from mutations of nuclear DNA follow Mendelian inheritance patterns (e.g. autosomal recessive).
In healthy tissue, all cells theoretically receive identical (wild type) mtDNA from their parental cell, which is referred to as homoplasmy. However, in the case of an mtDNA mutation, not all offspring cells receive an equal amount of mutated mtDNA (fig. 2). During cell division, wildtype and mutant mtDNA from parental cells are distributed randomly among daughter cells. Tissues that consist of cells that carry both mutated and wildtype mtDNA are in a state referred to as heteroplasmy. The degree of heteroplasmy influences tissue function, and once the mutant load passes a certain threshold, tissue function may become compromised. This so called ‘threshold effect’ varies in different tissue types, with a lower threshold for disease expression in tissues with higher energy demands, such as the central nervous system, skeletal and cardiac muscle, pancreatic islets, liver, and kidney [Boulet et al., 1992; Spinazzola, 2011]. This helps explain the common pattern of clinical manifestations in mitochondrial disease [White et al., 1999; Thorburn and Dahl, 2001; Wallace, 2001].
|Fig. 2. Key features and concepts involved in mitochondrial inheritance. A maternal cell may be heteroplasmic for mutant mtDNA and wildtype mtDNA. During mitotic segregation, mutant and wildtype mtDNA are distributed randomly among the daughter cells, resulting in a distribution of the degree of heteroplasmy in daughter cells. Selective mtDNA replication and amplification further increases the level of heteroplasmy in mature oocytes. As only oocytes contribute mitochondria to the zygote, the level of mutant mtDNA depends on the mtDNA mutant load inherited from the mother. If the mutant load remains below a tissue-specific threshold, the offspring may not demonstrate a detectable disease phenotype. However, depending on the proportion of mutant mtDNA, the offspring may show a variable phenotype, with the severity of manifestations as least partially related to the mutant load.|
Mitochondrial dysfunction can manifest in many organ systems and can be challenging to diagnose. We will outline key tests here, (as well as examples of how these tests may also show the presence of certain ‘classic’ biochemical disorders) though this brief overview is not intended to be comprehensive.
The initial laboratory evaluation in suspected mitochondrial disease may include pyruvate and lactate levels, as well as lactate/pyruvate ratio, blood urea nitrogen, glucose, plasma amino acids, urine organic acids, ammonia levels, serum electrolytes with anion gap, and acylcarnitine profile with total and free carnitine levels. Elevated lactate with a lactate/pyruvate ratio of 10–20 may indicate a disorder of pyruvate metabolism, such as can occur in PDH deficiency; an elevated lactate level with a lactate/pyruvate ratio >20 may indicate an OXPHOS disorder. Low blood urea nitrogen and high ammonia levels can be found in urea cycle defects. Abnormal blood glucose and ketone levels in addition to specific acylcarnitine, plasma amino acid and urine organic acid profiles can point to specific fatty acid oxidation defects, aminoacidopathies and organic acidurias, respectively. Skin or muscle biopsies can be used for histological (such as electron microscopy) or biochemical studies of mitochondria. Histochemical studies are helpful to evaluate the respiratory chain function, and genetic testing can help identify mtDNA or nuclear DNA mutations. For more details about testing for mitochondrial disorders, please refer to the review by Debray et al. .
VACTERL Association and Mitochondrial Dysfunction: Possibly Commonalities
There are interesting overlaps between VACTERL association and mitochondrial disease. Clinically, both share involvement of multiple disparate organs, high variability of disease expression (even within families with multiple affected individuals) and apparently multiple modes of inheritance. There are several reports of individuals with VACTERL association who developed manifestations consistent with mitochondrial dysfunction (though not all reported instances have been proven to involve mitochondrial dysfunction) [Solomon et al., 2010b, c; Solomon, 2011]. Though it would likely be an oversimplification to posit that mitochondrial dysfunction plays a frequent direct causative role in VACTERL association, links between the 2 classes of disorders remain interesting. For example, defective mitochondria, randomly distributed to different organ systems, may help explain why disparate tissues are affected in some instances of VACTERL association, and varying levels of heteroplasmy may explain the broad spectrum of disease seen in both sporadically affected individuals as well as kindreds with multiple affected members [Solomon et al., 2010b, c]. Further, as described below, there is a strong biological argument by which mitochondrial dysfunction could plausibly result in the pattern of congenital anomalies seen in VACTERL association.
VACTERL Association and Mitochondrial Dysfunction: Reported Cases
VACTERL association and mitochondrial disorders have been largely regarded as 2 distinct medical entities. Classically, VACTERL association was categorized as related to inborn errors of development, whereas mitochondropathies are typically grouped within or alongside the inborn errors of metabolism (IEM). However, there are a small number of case reports of individuals with both VACTERL association and mitochondrial dysfunction.
In summary, to date, 6 cases of proven mitochondrial dysfunction in patients with VACTERL association have been described in the literature [reviewed in Solomon et al., 2011]. Interestingly, 5 of the 6 affected individuals demonstrated complex IV deficiencies [Solomon et al., 2011], whereas one individual had an np 3243 A>G mitochondrial DNA point mutation. This latter patient presented with mitochondrial encephalopathy, lactic acidosis and stroke (MELAS-syndrome) [Damian et al., 1996]. All described individuals presented with features of VACTERL association as well as occasional additional minor anomalies not classically described as part of VACTERL association, such as facial dysmorphisms, pyloric stenosis and hypermobility [Solomon et al., 2011]. Overall, 5 of the 6 individuals presented with vertebral anomalies; 3 had anal atresia; 4 showed esophageal involvement; 2 had limb anomalies; 1 had renal anomalies; and 2 patients presented with additional minor dysmorphic features.
Multiple additional individuals with VACTERL association and strong clinical evidence of mitochondrial dysfunction have been reported – these individuals were initially described as having VACTERL association and later demonstrated features commonly seen in mitochondrial disorders, including characteristic patterns of cardiac, neurologic, gastrointestinal, and endocrine dysfunction. However, due to the frequent need of invasive testing and the diagnostic difficulties involved in proving mitochondrial disease, it is important to note that not all of these latter individuals had proven mitochondrial dysfunction, and thus, the connection may be spurious in some instances [Solomon et al., 2011] (table 1).
|Table 1. Summary of clinical findings in individuals with VACTERL association and strong evidence for mitochondrial disease|
Further Extrapolations: The Role of Mitochondria in Embryonic Development and Human Disease
As mitochondria provide the major source of energy (the OXPHOS system is the major source of energy, in the form of ATP; importantly, this system also produces reactive oxygen species/free radicals) for most mammalian cellular processes, including embryogenesis, it is unsurprising that primary and secondary mitochondrial dysfunction can have a significant impact on human development [Lemasters et al., 1999]. First, embryogenesis is an energy-dependent process, and ATP availability is necessary for normal tissue development and organization [Stone and Biesecker, 1997; von Kleist-Retzow et al., 2003]. Animal models show that the period of embryogenesis is particularly vulnerable to mitochondrial dysfunction due to high energy demands as well as related results of aberrant mitochondrial function (see below) [Saijo et al., 1997; Li et al., 2000; Johnson et al., 2001; Dumollard et al., 2007]. Thus, a lack of necessary energy (e.g. due to respiratory chain disease or mutations of pyruvate dehydrogenase-, cytochrome c-, or dihydrolipoamide dehydrogenase-encoding genes) may be detrimental to the developing embryo and lead to congenital anomalies [Saijo et al., 1997; Li et al., 2000; Johnson et al., 2001].
Second, under normal conditions, the respiratory chain generates approximately 2% of free radicals from its electrons. This percentage increases when electron transfer becomes dysfunctional. Free radicals/reactive oxygen species can cause oxidative damage (to both cells and genetic material); once innate antioxidant defense mechanisms are overwhelmed, this can result in apoptotic or necrotic cell death [Kroemer and Reed, 2000; Kowaltowski et al., 2009], leading to abnormal embryonic development and the clinical expression of congenital malformations [Van Blerkom, 2009]. Related to this, aberrant or dysregulated apoptosis induced by dysfunctional mitochondria may further trigger the apoptosis of surrounding cells (this phenomenon is commonly known as the bystander effect), further interfering with normal development. Additionally, the resulting malformations may involve a vicious cycle in which mitochondrial dysfunction begets oxidative stress, causing damage to the same respiratory chain deficient cells that show increased vulnerability to the same oxidative stress for which they are responsible [Yanicostas et al., 2011].
Relevant developmental malformations have been described in several animal studies. An antisense morpholino zebrafish model for the surf1, coxaa, and coxab genes (encoding the cytochrome c oxidase enzyme subunits) has a consistent phenotype that includes structural malformations. In this model, apoptosis is dramatically higher in tissues in which malformations occur, whereas the effects on other tissues (such as the heart) seem to be more related to energy deficiency [Baden et al., 2007].
In human studies, one case series described congenital anomalies in patients with confirmed mitochondrial dysfunction secondary to respiratory chain disease/ OXPHOS dysfunction; these patients demonstrated a wide spectrum of malformations involving multiple unrelated organ systems. Reported malformations included dysmorphic craniofacial features, cardiac malformations, limb anomalies, genitourinary anomalies, and gastrointestinal malformations [von Kleist-Retzow et al., 2003] (table 2 and fig. 3).
|Table 2. Summary of antenatal/postnatal manifestations of mitochondrial respiratory chain deficiencies in case series and single case report|
|Fig. 3. Involvement of multiple organ systems in mitochondrial disorders as well as related inborn errors of metabolism. Organ systems underlined/in bold indicate those in which there is most frequently a clinical overlap between individuals with VACTERL association and mitochondrial disorders/related IEM.|
Further Connections with Inborn Errors of Metabolism
To extend the discussion further to other biologically related disorders, it is important to point out that normal mitochondrial function involves more than the respiratory chain mechanism: the mitochondria house a variety of enzymes involved in the metabolism of amino acids, carbohydrates and lipids. Certain IEM can be caused by deficient activity of enzymes related to these metabolic pathways, such as in mitochondrial fatty acid oxidation disorders, urea cycle disorders, amino acid metabolism defects resulting in organic aciduria, pyruvate metabolism disorders, and tricarboxylic acid cycle disorders. Mitochondrial dysfunction secondary to toxic metabolite accumulation is a key feature of another group of IEM, namely organic acidurias (e.g. fumaric aciduria, 3-methylglutaconic aciduria and glutaric aciduria). Organic acidurias are caused by deficient enzyme function in the metabolism of coenzyme A activated carboxylic acids. These carboxylic acids are mainly derived from amino acid catabolism but can also come from defective mitochondrial lipid metabolism or as products of the tricarboxylic acid cycle [Goodman, 1980; Goodman and Markey, 1981; Chalmers and Lawson, 1982; Scriver et al., 2001; Wajner and Goodman, 2011].
Endogenous organic acid accumulation can perturb mitochondrial homeostasis by directly inhibiting OXPHOS consequent energy production [Cheema-Dhadli et al., 1975; Gregersen, 1981; Evangeliou et al., 1985; Massoud and Leonard, 1993; Okun et al., 2002; Baumgartner et al., 2007] or indirectly, via decreased expression of mtDNA of the electron transfer complexes I–IV [Mardach et al., 2005; Schwab et al., 2006; Sperl et al., 2006; de Keyzer et al., 2009]. Further, abnormal mitochondrial structure has also been observed, especially in the skeletal muscle, heart and liver in patients with organic acidurias [Mardach et al., 2005; Schwab et al., 2006; de Keyzer et al., 2009; Wortmann et al., 2009].
Patients affected with these IEM frequently present with neurologic manifestations, sometimes accompanied by additional findings more commonly associated with conditions involving structural malformations, the underlying pathogenesis of which is incompletely understood. However, growing evidence has emerged indicating that mitochondrial dysfunction is directly or indirectly involved in clinical manifestations such as the congenital malformations that are reported in some individuals with IEM. A key question relates to the relative paucity of congenital malformations in patients with IEM, begging the question whether such co-occurences may be largely coincidental. One explanation why less IEM present with congenital malformations may involve the fact that most IEM are compensated for in utero by maternal/placental metabolism. According to this type of explanation, most toxic metabolites may be eliminated from fetal/embryonic circulation, delaying clinical manifestations until the postnatal period. Thus, the negative effects of IEM on embryogenesis may not affect mitochondrial dysfunction directly. On the other hand, clinical manifestations of mitochondrial dysfunction (in the form of structural malformations) due to direct deficient enzyme activity of the respiratory chain may, in rare instances, be more severe and may be detected even during the antenatal period [Nissenkorn et al., 2001; von Kleist-Retzow et al., 2003; Solomon et al., 2011].
To focus further on a few specific examples, congenital anomalies are frequently described in PDHc deficiency [Brown, 2005], glutaric aciduria type 2 and 3-methylglutaconic aciduria type IV [Goodman et al., 1983], pyruvate oxidase deficiency [Damian et al., 1996], and fumaric aciduria [Kerrigan et al., 2000; Shih and Mandell, 2006]. The spectrum of described anomalies is broad. Common features involve structural central nervous system (CNS) anomalies, including dysgenesis of the corpus callosum, polymicrogyria and pachygyria, and additional white matter dysplasia. Dysmorphic facial features observed in conjunction with CNS malformations have been described as bearing some resemblance to fetal alcohol syndrome, which can include a long philtrum, epicanthal folds and a short nose with anteverted nares as well as additional features such as frontal bossing and upslanting palpebral fissures [Saijo et al., 1997; Cormier-Daire et al., 1996; Nissenkorn et al., 2001; Brown, 2005; Shih and Mandell, 2006]. In individuals with mitochondrial dysfunction, Cormier-Daire et al.  reported craniofacial anomalies, including round face, high forehead, flat philtrum, epicanthal folds, low-set ears, short neck, and ear dysplasia suggestive of the features seen in individuals with CHARGE syndrome as well as the structural malformations described above affecting the limbs, gastrointestinal, genitourinary, and cardiac systems [Cormier-Daire et al., 1996]. Additional anomalies reported in individuals with IEM include genitourinary anomalies, inguinal and umbilical hernia, and brachydactyly as well as a spectrum of end-organ damage affecting multiple tissues (including the brain, liver and heart) [Goodman et al., 1983; Cormier-Daire et al., 1996; Saijo at al., 1997; Li et al., 2000; Johnson et al., 2001; Nissenkorn et al., 2001; Rudolph et al., 2002; De Meirleir, 2005; Shih and Mandell, 2006; Wajner and Goodman, 2011].
The Contribution of Teratogens
Teratogens have long been known to have a major impact on embryonic development, and a group of agents, including lead [Levine and Muenke, 1991; Wang et al., 2009], glycosides [Marx et al., 2006], ethanol [Henderson et al., 1999], and maternal diabetes [Loffredo et al., 2001], have been reported as being associated with VACTERL association and are additionally known to impair mitochondrial function. This impairment of mitochondrial function can involve alterations of mitochondrial ultrastructure, interfering with respiratory chain function and exacerbating oxidative stress through increased production of free radicals. Oxidative stress may cause damage to many organs, including the heart, lung, liver, kidneys, reproductive organs, and CNS [Ahamed and Siddiqui, 2007] (table 3a, b).
This putative link between VACTERL association, teratogens and mitochondrial dysfunction raises the question whether early prenatal exposure to certain teratogens induces mitochondrial dysfunction or at least exacerbates preexisting mitochondrial dysfunction in heteroplasmic tissues, where the proportion of dysfunctional mitochondria is initially below the threshold. Teratogens may act as an additional stressor on the already vulnerable heteroplasmic tissues, thereby rendering more mitochondria dysfunctional. In this ‘susceptibility model’, a combination of factors would ultimately cause the overall proportion of impaired mitochondria supercede tissue-specific functional thresholds.
VACTERL association is a clinically and causally heterogenous disorder, and the overall mechanism of disease remains incompletely understood. The involvement of many apparently unrelated organ systems, as well as the lack of a definite Mendelian inheritance mode in the majority of instances, suggests the presence of new, currently unidentified mutations in some individuals, as well as the possibility of a more complex multifactorial disorder in other individuals, with genetic, epigenetic, environmental, and more purely stochastic factors combinatorially involved in disease pathogenesis.
To date, no common causal link between mitochondrial dysfunction and the development of VACTERL association has been demonstrated. Mitochondrial dysfunction might not be the sole cause for the vast majority of individuals with VACTERL association, but could play a role through increasing tissue susceptibility to aberrant development. Additionally, the recognition of the involvement of mitochondrial dysfunction in the development of congenital anomalies through impaired energy production, reactive oxygen species generation and aberrant apoptosis in well-established disorders opens the door for further discussion, investigation and research in the fast developing field of complex disorders, including explanations of variable disease expression in some Mendelian conditions. For example, even in well-described genetic disorders, such as Down syndrome, which can include multiple congenital anomalies of the cardiac, central nervous and gastrointestinal systems [Solomon et al., 2010a], mitochondropathies have been posited as disease modifier. Mitochondrial dysfunction due to mtDNA mutations, mitochondrial enzyme deficiencies leading to altered patterns of apoptosis and increased oxidative stress, has been reported as playing a role in the clinical manifestations of Down syndrome [Busciglio et al., 2002; Busciglio, 2010; Tiano and Busciglio, 2011], for example, by affecting the frequency of diabetes mellitus and Alzheimer disease, altering the immune system, and contributing to neurological effects [Prince et al., 1994; Bersu et al., 1998; Druzhyna et al., 1998; Schuchmann and Heinemann, 2000; Conti et al., 2007; Roat et al., 2007].
In summary, we suggest that mitochondrial dysfunction should be considered when a patient presents with multiple congenital anomalies and clinical signs, and symptoms of mitochondrial involvement or related IEM. These clinical clues can include evidence for a maternal inheritance pattern, abnormal glycemic control, cyclic vomiting, developmental delay and other related signs of neurological disease, dysautonomia, intestinal malabsorption, pancreatic insufficiency, ptosis, episodic vomiting, and clinical response to ‘mitochondrial cocktail’ [Solomon et al., 2011].
Finally, it must be admitted that past critiques have pointed out that mitochondrial defects have been the default explanation for many inexplicable conditions. Others believe that mitochondria may, however, play a unifying role in a large and underappreciated number of medically significant conditions. DiMauro  has even called mitochondrial mutations a ‘Pandora’s box’, perhaps reminding us of the difficulties in understanding and interpreting the many types of mitochondrial dysfunction and their potentially devastating consequences. At the same time, we must stride for means to discover new and better treatments for the many manifestations of mitochondrial disease. Despite the diversity of opinions, the bottom line is that much more research and critical evaluation is required in order to find answers for individuals affected by either or both VACTERL association and mitochondrial disease. This is especially important as some metabolic and mitochondrial disorders are amenable to certain types of treatment, and key avenues may emerge in the study of VACTERL association and related conditions related to mechanisms to ameliorate disease severity. As the causes of VACTERL association are discovered, it will be fascinating to look back in order to determine whether identified molecular etiologies do need point to a common path involving the mitochondria.
This research was supported by the Division of Intramural Research, National Human Genome Research Institute (NHGRI), National Institutes of Health and Human Services, USA. B.D.S. would like to thank Max Muenke, MD, for his support and mentorship. The authors would like to thank Lynne Wolfe, MS, CRNP, for her helpful insights and discussion, and Julia Fekecs and Darryl Leja for their expert illustrations, and are extremely grateful to the patients and families who participate in our studies.
Benjamin D. Solomon, MD
NIH, MSC 3717
Building 35, Room 1B-207
Bethesda, MD 20892 (USA)
Published online: January 11, 2013
Number of Print Pages : 11
Number of Figures : 3, Number of Tables : 3, Number of References : 103
Vol. 4, No. 1-2, Year 2013 (Cover Date: February 2013)
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ISSN: 1661-8769 (Print), eISSN: 1661-8777 (Online)
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