Journal Mobile Options
Table of Contents
Vol. 34, No. 1, 2002
Issue release date: January–February 2002

Gene Expression of Neurotrophins and Their High-Affinity Trk Receptors in Cultured Human Müller Cells

Oku H. · Ikeda T. · Honma Y. · Sotozono C. · Nishida K. · Nakamura Y. · Kida T. · Kinoshita S.
To view the fulltext, log in and/or choose pay-per-view option

Individual Users: Register with Karger Login Information

Please create your User ID & Password

Contact Information

I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in


Purpose: To investigate the gene expression of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT-3) and their high-affinity receptors (TrkA, TrkB and TrkC) in cultured human Müller cells. Methods: The polymerase chain reaction was performed using specific primers for NGF, BDNF, NT-3, TrkA, TrkB and TrkC with complementary DNAs as the template, which were generated from poly-A+ RNA of cultured human Müller cells. Results: We detected the precursor mRNA transcripts for NGF, BDNF, NT-3, TrkB and TrkC, but not TrkA. Conclusion: Human Müller cells can direct mRNA expressions of a number of neurotrophins which may have a neurotrophic function in the retina. An autocrine mode of action is suggested, since Müller cells not only synthesize neurotrophic factors, but also express their specific receptors.

Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.


  1. Barbacid M: The Trk family of neurotrophin receptors. J Neurobiol 1994;25:1386–1403.
  2. Müller HW, Beckh S, Seifert W: Neurotrophic factor for central neurons. Proc Natl Acad Sci USA 1984;81:1248–1252.
  3. Schmalenbach C, Müller HW: Astroglia-neuron interactions that promote long-term neuronal survival. J Chem Neuroanat 1993;6:229–237.
  4. Rohrer B, Korenbrot JI, LaVail MM, Reichardt LF, Xu B: Role of neurotrophin receptor TrkB in the maturation of rod photoreceptors and establishment of synaptic transmission to the inner retina. J Neurosci 1999;19:8919–8930.

    External Resources

  5. Wahlin KJ, Campachiaro PA, Zack DJ, Adler R: Neurotrophic factors cause activation of intercellular signaling pathways in Müller’s cells and other cells of the inner retina, but not photoreceptors. Invest Ophthalmol Vis Sci 2000;41:927–935.
  6. Harada T, Harada C, Nakayama N, Okuyama S, Yoshida K, Kohsaka S, Matsuda H, Wada K: Modification of glial-neuronal cell interactions prevents photoreceptor apoptosis during light-induced retinal degeneration. Neuron 2000;26:533–541.
  7. Puro DG, Mano T, Chan CC, Fukuda M, Shimada H: Thrombin stimulates the proliferation of human retinal glial cells. Graefes Arch Clin Exp Ophthalmol 1990;228:169–173.
  8. Puro DG, Mano T: Modulation of calcium channels in retinal glial cell by basic fibroblast growth factor: A possible role in retinal pathology. J Neurosci 1991;11:1873–1880.

    External Resources

  9. Ikeda T, Puro DG: Nerve growth factor: A mitogenic signal for retinal Müller glial cells. Brain Res 1994;27:260–264.
  10. Nishida K, Sotozono C, Adachi W, Yamamoto S, Yokoi N, Kinoshita S: Transforming growth factor-β1, -β2 and -β3 mRNA expression in human cornea. Curr Eye Res 1995;14:235–241.
  11. Saiki RS, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N: Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 1985;230:1350–1354.
  12. Ercolani L, Florence B, Denaro M, Alexander M: Isolation and complete sequence of a functional human glyceraldeyhde-3-phosphate dehydrogenase gene. J Biol Chem 1988;263:15335–15341.
  13. Hirsch S, Labes M, Bahr M: Changes in BDNF and neurotrophin receptor expression in degeneration and regeneration rat retinal ganglion cell. Restor Neurol Neurosci 2000;17:125–134.
  14. Unoki K, LaVail MM: Protection of the rat retina from ischemic injury by brain-derived neurotrophic factor, ciliary neurotrophic factor and basic fibroblast growth factor. Invest Ophthalmol Vis Sci 1994;35:907–915.
  15. Peinado-Ramon P, Salvador M, Villegas-Perez MP, Vidal-Sanz M: Effects of axotomy and intraocular administration of NT-4, NT-3, and brain derived neurotrophic factor on the survival of adult rat retinal ganglion cells: A quantitative in vivo study. Invest Ophthalmol Vis Sci 1996;37:489–500.
  16. Lewis GP, Linberg KA, Geller SF, Guerin CJ, Fisher SK: Effects of the neurotrophin brain-derived neutrotrophic factor in an experimental model of retinal detachment. Invest Ophthalmol Vis Sci 1999;40:1530–1544.
  17. Johnson EC, Deppmeier LM, Wentzien SK, Hsu I, Morrison JC: Chronology of optic nerve head and retinal responses to elevated intraocular pressure. Invest Ophthalmol Vis Sci 2000;41:431–442.
  18. Ikeda T, Puro DG: Regulation of retinal glial cell proliferation by antiproliferative molecules. Exp Eye Res 1995;60:435–443.
  19. Bignami A, Dahl D: The radial glia of Müller in the rat retina and their response to injury: An immunofluorescence study with antibodies to the glial fibrillary acidic (GFA) protein. Exp Eye Res 1979;28:63–69.

    External Resources

  20. Osborne NN, Block F, Sontag KH: Reduction of ocular blood flow results in glial fibrillary acidic protein (GFAP) expression in rat retinal Müller cells. Vis Neurosci 1991;7:637–639.
  21. Honjo M, Tanihara H, Kido N, Inatani M, Okazaki K, Honda Y: Expression of ciliary neurotrophic factor activated by retinal Müller cells in eyes with NMDA- and kainic acid-induced neuronal death. Invest Ophthalmol Vis Sci 2000;41:552–560.

    External Resources

  22. Wagner N, Wagner KD, Sefton M, Rodriges-Tebar A, Grantyn R: An abnormal response of retinoblastoma cells (Y-79) to neurotrophins. Invest Ophthalmol Vis Sci 2000;41:1932–1939.
  23. Kume T, Nishikawa H, Tomioka H, Katsuki H, Akaike A, Saneko S, Maeda T, Kihara T, Shimohama S: p-75-mediated neuroprotection by NGF against glutamate cytotoxicity in cortical cultures. Brain Res 2000;852:279–289.

    External Resources

Pay-per-View Options
Direct payment This item at the regular price: USD 38.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 26.50