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Vol. 64, No. 1, 2001
Issue release date: 2001
Section title: Original Paper: Esophagus
Digestion 2001;64:1–8
(DOI:10.1159/000048833)

Dilated Intercellular Spaces as Markers of Reflux Disease: Histology, Semiquantitative Score and Morphometry upon Light Microscopy

Villanacci V. · Grigolato P.G. · Cestari R. · Missale G. · Cengia G. · Klersy C. · Rindi G.
aPathology Service 2, bDepartment of Surgery (Digestive Endoscopy), University of Brescia and Spedali Civili, Brescia, and cBiometric and Clinical Epidemiology Service, IRCCS Policlinico San Matteo, Pavia, Italy

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Article / Publication Details

First-Page Preview
Abstract of Original Paper: Esophagus

Published online: 9/10/2001

Number of Print Pages: 8
Number of Figures: 2
Number of Tables: 3

ISSN: 0012-2823 (Print)
eISSN: 1421-9867 (Online)

For additional information: http://www.karger.com/DIG

Abstract

Background and Aims: A recent electron microscopy study suggested that dilated intercellular spaces (DIS) are specific for acid reflux-damaged esophageal epithelium. Electron microscopy is, however, expensive and difficult to apply to routine biopsies. The aims of this study are to establish a method for assessing DIS on light microscopy of esophageal biopsies and to estimate its association with current clinicopathological parameters of esophagitis. Materials and Methods: 21 patients with reflux symptoms were investigated. Light microscopy biopsies were assessed for DIS size by a semiquantitative method and computer-assisted, static morphometry. A DIS score accounting for DIS size and distribution was assigned to each patient and its association with 30 clinicopathological variables investigated by univariate and multivariate logistic regression. Results: Both the semiquantitative method and static morphometry identified 4 different classes of DIS size. The DIS score was significantly and independently associated with the esophageal symptoms score, the histological score of esophagitis and the relevant morphometry data. Conclusions: DIS may be efficiently assessed during light microscopy of routine esophageal biopsies. Since correlation with both the histology and the symptoms of esophagitis, the DIS score may be considered a novel parameter of esophagitis and is suggested for the routine evaluation of esophageal biopsies in patients with reflux disease.


Article / Publication Details

First-Page Preview
Abstract of Original Paper: Esophagus

Published online: 9/10/2001

Number of Print Pages: 8
Number of Figures: 2
Number of Tables: 3

ISSN: 0012-2823 (Print)
eISSN: 1421-9867 (Online)

For additional information: http://www.karger.com/DIG


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Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
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    External Resources

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