Journal Mobile Options
Table of Contents
Vol. 52, No. 2, 2001
Issue release date: September 2001
Gynecol Obstet Invest 2001;52:104–107
(DOI:10.1159/000052952)

Modulatory Effect of Acetylcholine on Gonadotropin-Stimulated Human Granulosa Cell Steroid Secretion

Kornya L. · Bódis J. · Koppán M. · Tinneberg H.R. · Török A.
Departments of Obstetrics and Gynecology,aPeterfy Hospital, Budapest, Hungary, bBaranya County Teaching Hospital of Pécs, Hungary and cCity Hospital of Bielefeld, Germany

Individual Users: Register with Karger Login Information

Please create your User ID & Password





Contact Information











I have read the Karger Terms and Conditions and agree.

To view the fulltext, please log in

To view the pdf, please log in

Abstract

The aim of this study was to explore the direct action of acetylcholine on gonadotropin-stimulated progesterone (P) and estradiol (E2) secretion of human granulosa cells (GCs) cultured in serum-free medium. Human GCs were isolated from preovulatory follicular fluid aspirated from 22 women undergoing in vitro fertilization at the University Women’s Hospital of Tübingen. The production of progesterone and E2 was measured in the presence and absence of acetylcholine, carbachol, atropine, luteinizing hormone (LH) or follicle-stimulating hormone (FSH) using radioimmunoassay. Statistical analysis of the data was performed by ANOVA and Newman-Keuls test. Administration of acetylcholine or carbachol (10–5M) resulted in a significant increase in P and E2 secretion. This response was specifically blocked by the muscarinic receptor antagonist atropine. Similarly, carbachol resulted in a significant increase in P and E2 output, though the response to it was somewhat reduced when compared to that evoked by acetylcholine. Acetylcholine did not show any additive effect on LH-stimulated P secretion, while it augmented the stimulatory effect of FSH on P release. In contrast, carbachol markedly diminished the stimulatory effect of LH on P secretion, while it caused no change in FSH-induced P output. When administered together, acetylcholine did not modify the stimulatory effect of FSH on E2 secretion, however, it markedly elevated LH-induced E2 output. Similar to this, carbachol significantly increased LH-induced E2 release, however it decreased FSH-stimulated E2 secretion. We suggest that acetylcholine has a direct modulatory effect on gonadotropin-stimulated steroid production of GCs, an effect that is mediated via muscarinic receptors. This effect may have a physiological role in the regulation of GC function during the menstrual cycle.



Copyright / Drug Dosage

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

References

  1. Luck M: Cholinergic stimulation, through muscarinic receptors, of oxytocin and progesterone secretion from bovine granulosa cells undergoing spontaneous luteinization in serum-free culture. Endocrinology 1990;126:1256–1263.

    External Resources

  2. Bódis J, Tinneberg HR, Papenfuss F, Török A, Cledon PH, Hanf V, Schwarz H: Cholinergic stimulation of progesterone and estradiol secretion by human granulosa cells cultured in serum-free medium. Gynecol Endocrinol 1993;7:83–87.
  3. Papenfuss F, Bódis J, Tinneberg HR, Schwarz H: The modulatory effect of catecholamines on gonadotropin-stimulated granulosa cell steroid secretion. Arch Gynecol Obstet 1993;253:97–102.
  4. Burden HW: Adrenergic innervation in ovaries of the rat and guinea pig. Am J Anat 1972;133:455–462.

    External Resources

  5. Burden HW, Lawrence IE: Experimental studies on the acetylcholinesterase-positive nerves in the ovary of the rat. Anat Rec 1978;190:233–242.

    External Resources

  6. Bahr JM, Ben-Jonathan N: Pre-ovulatory depletion of ovarian catecholamine. Endocrinology 1981;108:1815–1820.

    External Resources

  7. Bahr JM, Kaeo L, Nalbanov AV: The role of catecholamine and nerves in ovulation. Biol Reprod 1974;10:273–290.

    External Resources

  8. Aguado LI, Petrovic SL, Ojeda SR: Ovarian β-adrenergic receptors during the onset of puberty: Characterization, distribution and coupling to steroidogenic responses. Endocrinology 1982;110:1124–1132.

    External Resources



Pay-per-View Options
Direct payment This item at the regular price: USD 33.00
Payment from account With a Karger Pay-per-View account (down payment USD 150) you profit from a special rate for this and other single items.
This item at the discounted price: USD 23.00