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Vol. 125, No. 4, 2001
Issue release date: August 2001

Allergy to Dermatophagoides in a Group of Spanish Gypsies: Genetic Restrictions

Cárdaba B. · Moffatt M.F. · Fernández E. · Jurado A. · Rojo M. · García M. · Ansotegui I.J. · Cortegano I. · Arrieta I. · Etxenagusia M.A. · del Pozo V. · Urraca J. · Aceituno E. · Gallardo S. · Palomino P. · Cookson W. · Lahoz C.
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Abstract

Background: Spanish gypsies have traditionally lived as nomads, a reason why few epidemiological studies were done in this ethnic group. However, the high prevalence of asthmatic diseases demonstrated in a population residing in the North of Spain induces us to analyse whether it was due to the influence of genetic loci previously implicated in other population studies as causing the disorders. Methods: DRB1* and DQB1* HLA class II, TCR-Vα8.1, FcεRI-β Rsa I exon 7 and intron 2, TNF-β (LTα-Nco I) and CD14, were tested for association with asthma and atopy by multiple regression analysis, in 5 families comprising 87 individuals. Results: Significant associations were found with DQB1*02 (p = 0.02) and DQB1*0301 (p = 0.008) and elevated levels of total serum IgE. A negative association (p = 0.02) was found between total serum IgE and DRB1*14. FcεRI-β Rsa I-In2 allele 1 was associated with high levels of total serum IgE (p = 0.04). Levels of Der p 1 IgE antibodies were negatively associated with DRB1*11-DQB1*0301 (p = 0.007), and positively with TCR Vα-8 allele 1 (p = 0.04) and with FcεRI-β Rsa I-In2 allele 1 (p = 0.009). Conclusions: Our results do not show any association between asthma and the genetic loci studied although they do suggest the existence of multiple genetic influences on the allergic response in these families.



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References

  1. Burney P, Malberg E, Chinn S, Jarvis D, Luczunska C, Lai E: The distribution of total and specific serum IgE in the European Community Respiratory Health Survey. J Allergy Clin Immunol 1997;99:314–322.
  2. Ford SA, Tovey ER, Baldo BA: The spectrum of low molecular weight house dust mite (Dermatophagoides pteronyssinus) allergens with emphasis on Der p II. Clin Exp Allergy 1989;20:27–31.
  3. Thompson SJ, Withley HJ, Naysmith JD, Carswell F: IgE antibodies to D. pteronyssinus in atopic patients. Immunology 1988;64:311–314.
  4. Sporik R, Holgate ST, Platts-Mills TA, Cogswell JJ: Exposure to house-dust mite allergen (Der p 1) and the development of asthma in childhood. N Engl J Med 1990;323:502–507.
  5. Cortes X, Soriano JB, Sánchez-Ramos JL, Azofra J, Almar E, Ramos J: European study of asthma. Prevalence of atopy in young adults of 5 areas in Spain. Spanish Group of European Asthma Study. Med Clin 1998;111:573–577.
  6. Marsh DG, Hsu SH, Roebber M, Kautzka EE, Friedhoff LR, Meyers DA, Pollard MK, Bias WB: HLA-Dw2: A genetic marker for human immune response to short ragweed pollen allergen Ra5. I. Response resulting primarily from natural antigenic exposure. J Exp Med 1982;155:1439–1451.
  7. Fisher GF, Pickl WF, Faé I, Ebner C, Ferreira F, Breiteneider H, Vikoukal E, Scheiner O, Kraft D: Association between IgE response against Bet v I, the major allergen of birch pollen and HLA-DRB alleles. Hum Immunol 1992;33:259–265.
  8. Cárdaba B, Vilches C, Martín E, de Andrés B, del Pozo V, Hernández D, Gallardo S, Fernández JC, Villalba M, Rodríguez R, Palomino P, Kreisler M, Lahoz C: DR7 and DQ2 are positively associated with immunoglobulin-E response to the main antigen of olive pollen (Ole e 1) in allergic patients. Hum Immunol 1993;38:293–299.
  9. Moffatt MF, Hill MR, Cornélis F, Schou C, Faux JA, Young RP, James AL, Ryan G, le Souëf P, Musk AW, Hopkin JM, Cookson WOCM: Genetic linkage of the TCR-α/δ region to specific Immunoglobulin E responses. Lancet 1994;343:1597–2000.
  10. Cookson WCOM, Sharp PA, Faux JA, Hopkin JM: Linkage between immunoglobulin E responses underlying asthma and rhinitis and chromosome 11q. Lancet 1989;i:1292–1295.
  11. Marsh DG, Neely JD, Breazeale DR, Ghosh B, Freidhoff LR, Ehrlich-Kautzky E, Schou C, Krishnaswamy G, Beaty TH: Linkage analysis of IL4 and other chromosome 5q31.1 markers and total serum immunoglobulin E concentrations. Science 1994;264:1152–1156.
  12. Barnes KC, Neely JD, Duffy DL, Freidhoff LR, Breazeale DR, Schou C, Naidu RP, Levett PE, Renault B, Kucherlapati R, Iozzino S, Ehrlich E, Beaty TH, Marsh DG: Linkage of asthma and total serum IgE concentration to markers on chromosome 12q: Evidence from Afro-Caribbean and Caucasian populations. Genomics 1996;37:41–50.
  13. Baldini MI, Lohman C, Halonen M, Erikson RP, Holt PG, Martínez FD: A polymorphism in the 5′ flanking region of the CD14 gene is associated with circulating soluble CD14 levels and with total serum immunoglobulin E. Am J Respir Cell Mol Biol 1999;20:976–993.
  14. Standards for the diagnosis and care of patients with chronic obstructive pulmonary disease (COPD) and asthma. This official statement of the American Thoracic Society was adopted by the ATS Board of Directors, November 1986. Am Rev Respir Dis 1987;136:225–244.
  15. Miller SA, Dykes D, Polesky HK: A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988;16:1215–1218.
  16. Cornélis F, Pile K, Loveridge J, Moss P, Harding R, Julier C, Bell J: Systematic study of human αβ T cell receptor V segments shows allelic variations resulting in a large number of distinct T cell receptor haplotypes. Eur J Immunol 1993;23:1277–1283.

    External Resources

  17. Shirakawa T, Mao XQ, Sasaki S, Kawai M, Morimoto K, Hopkin J: Association between FcεRIβ and atopic disorder in a Japanese population. Lancet 1996;347:394–395.
  18. Palmer LJ, Paré PD, Faux JA, Moffatt MF, Daniels SE, LeSouëf PN, Bremner PR, Mockford E, Gracey M, Spargo R, Musk AW, Cookson WO: FcεRIβ polymorphism and total serum IgE levels in endemically parasitized Australian aborigines. Am J Hum Genet 1997;61:182–188.
  19. Messer G, Spengler U, Jung UC, Honold G, Blömer K, Pape GR, Riethmuller G, Weiss EH: Polymorphi structure of the tumor necrosis factor (TNF) locus: An Nco I polymorphism in the first intron of the human TNF-β gene correlates with a variant amino acid in position 26 and a reduced level of TNF-β production. J Exp Med 1991;173:209–219.
  20. Cárdaba B, Cortegano I, Florido F, Arrieta I, Aceituno E, del Pozo V, Gallardo S, Rojo M, Palomino P, Lahoz C: Genetic restrictions in olive pollen allergy. J Allergy Clin Immunol 2000;105:292–298.
  21. Davis S, Schroeder M, Goldin LR, Weeks DE: Non-parametric simulation-based statistics for detecting linkage in general pedigrees. Am J Hum Genet 1996;867–880.
  22. Davis S, Goldin LR, Weeks DE: SimIBD: A powerful robust non-parametric method for detecting linkage in general pedigrees; in Palowitzki I-H, Edwards JH, Thompson EA (eds): Genetic Mapping of Disease Genes. New York, Academic Press, 1997, pp 189–204.
  23. De Pablo R, Vilches C, Moreno ME, Rementería MC, Solís R, Kreisler M: Distribution of HLA antigens in Spanish Gypsies: A comparative study. Tissue Antigens 1992;40:187–196.

    External Resources

  24. Moffatt MF, Cookson WOCM: Genetics of asthma and inflammation: The status. Curr Opin Immunol 1999;11:606–609.

    External Resources

  25. García M, Fernández E, del Pozo MD, Navarro Ja, Gastaminza G, Olasagasti C: Prevalence of asthma and atopy in gypsy and non-gypsy population in a district of Vitoria-Gasteiz. Allergy 1995;26:160.
  26. Fernández E, García M, del Pozo MD, Díez J, Urraca J, Olasagasti C: Study of the characteristics of the dwelling of asthmatic and non-asthmatic individuals of gypsy and non-gypsy population of the district Sansomendi in Vitoria-Gasteiz, Spain. Allergy 1996;51:127.
  27. Hooloway JW, Doull I, Begishvili B, Beasley R, Holgate ST, Howell M: Lack of evidence of a significant association between HLA-DR, DQ and DP genotypes and atopy in families with HDM allergy. Clin Exp Allergy 1996;26:1142–1149.
  28. Torres-Galvan MJ, Quiralte J, Blanco C, Castillo T, Carrillo T, Pérez-Aciego P, Sánchez-García F: Linkage of house dust mite allergy with the HLA region. Ann Allergy Asthma Immunol 1999;82:198–203.

    External Resources

  29. Hizawa N, Collins G, Rafnar T, Huang SK, Duffy DL, Weber JL, Freidhoff LR, Ehrlich E, Marsh DG, Beaty TH, Barnes KC: Linkage analysis of Dermatophagoides pteronyssinus-specific IgE responsiveness with polymorphic markers on chromosome 6p21 (HLA-D region) in Caucasian families by the transmission/disequilibrium test. J Allergy Clin Immunol 1998;102:443–448.

    External Resources

  30. Stephan V, Kuehr J, Seibt A, Saueressig H, Zingsem S, Dinh TD, Moseler M, Wahn V, Deichmann KA: Genetic linkage of HLA-class II locus to mite-specific IgE immuno responsiveness. Clin Exp Allergy 1999;29:1049–1054.
  31. Higgins J, Thorpe CJ, Hayball JD, O’Heir RE, Lamb JR: Overlapping T cell epitopes in the group I allergen of Dermatophagoides species restricted by HLA-DP and -DR class II molecules. J Allergy Clin Immunol 1994;93:891–899.

    External Resources

  32. Verhoef A, Higgins JA, Thorpe CJ, Marsh SG, Hayball JD, Lamb JR, O’Hehir RE: Clonal analysis of the atopic immune repsonse to the group 2 allergen of Dermatophagoides spp. identification of HLA-DR and -DQ restricted epitopes. Int Immunol 1993;5:1589–1597.
  33. Lara-Márquez ML, Yuniss J, Layrisse Z, Ortega F, Carvallo-Gil E, Montagnani S, Makhatadze NJ, Pocino M, Granja C, Yunis E: Immunogenetics of atopic asthma: Association of DRBI*1101 DQA1*0501 DQβ1*0301 haplotype with Dermatophagoides spp.-sensitive asthma in a sample of the Venezuelan population. Clin Exp Allergy 1999;29:60–71.
  34. Hsia KH, Shien CC, Hsieh RP, Liu WJ: Association of HLA-DQw2 with Chinese chilhood asthma. Tissue Antigens 1991;38:181–182.
  35. Ogawa A, Tokunaga K, Lin L, Kashiwase K, Tanaka H, Herrero MJ, Vilches C, Park MH, Jia GJ, Chimge NO, Sideltsua EW, Ishihawz Y, Akaza T, Tadokoro K, Juji T: Diversity of HLA-B61 alleles and haplotypes in East Asians and Spanish Gypsies. Tissue Antigens 1998;51:356–366.
  36. Gerbase-DeLima M, Gallo CA, Daher S, Sole D, Naspitz CK: HLA antigens in asthmatic children. Pediatr Allergy Immunol 1997;8:150–152.
  37. Moffatt MF, Schou C, Faux JA, Cookson WOCM: Germline TCR-A restriction of immunoglobulin E response to allergen. Immunogenetics 1997;46:226–230.

    External Resources

  38. Virchow JC, Walker C, Hafner D, Kortisk C, Werner P, Mathys H, Kroegel C: T cells and cytokines in bronchoalveolar lavage fluid after segmental allergen provocation in atopic asthma. Am J Respir Crit Care Med 1995;51:960–968.
  39. Sandford AJ, Shirakawa T, Moffatt MF, Daniels SE, Ra C, Faux JA, Young RP, Nakamura Y, Lathrop GM, Cookson WO, et al: Localization of atopy and the beta subunit of the high-affinity IgE receptor (FcεRI) on chromosome 11q. Lancet 1993;341:332–334.


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