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Vol. 90, No. 3-4, 2000
Issue release date: 2000
Cytogenet Cell Genet 90:291–297 (2000)

Isolation, characterization and mapping of the mouse and human PRG4 (proteoglycan 4) genes

Ikegawa S. · Sano M. · Koshizuka Y. · Nakamura Y.
aLaboratory of Genome Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo; bLaboratory for Bone and Joint Diseases, SNP Research Center, RIKEN (The Institute of Physical and Chemical Research), Tokyo; cDepartment of Orthopaedic Surgery, Hamamatsu Medical School, Hamamatsu; dDepartment of Orthopaedic Surgery, Faculty of Medicine, The University of Tokyo, Tokyo (Japan)

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PRG4 (proteoglycan 4) has been identified as megakaryocyte stimulating factor and articular superficial zone protein. PRG4 has characteristic motifs including somatomedin B and hemopexin domains, a chondroitin sulfate-attachment site and mucin-like repeats. During a screen of genes implicated in ectopic ossification, we found a novel mouse gene highly homologous to human and bovine PRG4 genes. Here, we report isolation, characterization and mapping of the gene, Prg4 together with characterization of its human orthologue. Prg4 cDNA was 3,320 bp long, encoding a 1,054 amino-acid protein. Human and mouse PRG4 genes each consisting of 12 exons spanned 18 and 16 kb, respectively. Characteristic motifs were conserved across species; however, the mucin-like repeat regions were highly diverse in length between species with a tendency that larger animals had longer repeats. Expression of human and mouse PRG4 genes was similar and found not only in cartilage, but also in liver, heart, lung, and bone. Expression of the mouse gene increased with progression of ectopic ossification. Multiple tissue-specific splicing variants lacking some of the motifs were found in both human and mouse. Although a specific role in the articular joint has previously been reported, the presence of multi-functional motifs as well as unique expression and alternative splicing patterns suggest that PRG4 functions in several distinctive biological process including regulation of ossification.   

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  1. DeBry RW, Seldin MF: Human/mouse homology relationships. Genomics 33:337–351 (1996).

    External Resources

  2. Deng G, Curriden SA, Wang S, Rosenberg S, Loskutoff DJ: Is plasminogen activator inhibitor-1 the molecular switch that governs urokinase receptor-mediated cell adhesion and release? J Cell Biol 134: 1563–1571 (1996).
  3. Flannery CR, Hughes CE, Schumacher BL, Tudor D, Aydelotte M B, Kuettner KE, Caterson B: Articular cartilage superficial zone protein (SZP) is homologous to megakaryocyte stimulating factor precursor and is a multifunctional proteoglycan with potential growth-promoting, cytoprotective, and lubricating properties in cartilage metabolism. Biochem biophys Res Communi 254:535–541 (1999).
  4. Maruyama K, Sugano S: Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene 138:171–174 (1994).
  5. Merberg DM, Fitz LJ, Temple P, Giannotti J, Murtha P, Fitzgerald M, Scaltreto J, Kelleher K, Preissner K, Kriz R, Jacobs K, Turner K: A comparison of vitronectin and megakaryocyte stimulating factor, in Preissner KT, Rosenblatt S, Kost C, Wegerhoff J, Mosher DF (eds): Biology of Vitronectins and Their Receptors, pp 45–52 (Elsevier Science Publishers BV, New York 1993).
  6. Okawa A, Nakamura I, Goto S, Moriya H, Nakamura Y, Ikegawa S: Mutation in Npps in a mouse model of ossification of the posterior longitudinal ligament of the spine. Nature Genet 19:271–273 (1998).
  7. Schumacher BL, Block JA, Schmid TM, Aydelotte MB, Kuettner KE: A novel proteoglycan synthesized and secreted by chondrocytes of the superficial zone of articular cartilage. Arch Biochem Biophys 311:144–152 (1994).
  8. Schumacher BL, Hughes CE, Kuettner KE, Caterson B, Aydelotte MB: Immunodetection and partial cDNA sequence of the proteoglycan, superficial zone protein, synthesized by cells lining synovial joints. J Orthop Res 17:110–120 (1999).
  9. Seiffert D, Loskutoff DJ: Evidence that type 1 plasminogen activator inhibitor binds to the somatomedin B domain of vitronectin. J biol Chem 266:2824–2830 (1991).
  10. Seiffert D, Ciambrone G, Wagner NV, Binder BR, Loskutoff DJ: The somatomedin B domain of vitronectin. Structural requirements for the binding and stabilization of active type 1 plasminogen activator inhibitor. J biol Chem 269:2659–2666 (1994).
  11. Takahashi E, Hori T, O’Connell P, Leppert M, White R: R-banding and nonisotopic in situ hybridization: precise localization of the human type II collagen gene (COL2A1). Hum Genet 86:14–16 (1990).
  12. Turner KJ, Fitz LJ, Temple P, Jacobs K, Larson D, Leary AC, Kelleher K, Giannotti J, Calvetti J, Fitzgerald M, Kriz M J, Ferenz C, Grobholz J, Fraser H, Bean K, Norton CR, Gesner T, Bhatia S, Kriz R, Hewick R, Clark SC: Purification, biochemical characterization, and cloning of a novel megakaryocyte stimulating factor that has megakaryocyte colony stimulating activity. (Abstract). Blood 78: 279a (1991).
  13. van Driel IR, Goding JW: Plasma cell membrane glycoprotein PC-1: primary structure deduced from cDNA clones. J biol Chem 262:4882–4887 (1987).

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