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Vol. 92, No. 3-4, 2001
Issue release date: 2001
Cytogenet Cell Genet 92:337–341 (2001)
(DOI:10.1159/000056924)

cDNA cloning, chromosomal localization and evolutionary analysis of mouse vacuolar ATPase subunit D, Atp6m

Kennell J.A. · Richards N.W. · Schaner P.E. · Gumucio D.L.
Department of Cell and Developmental Biology, University of Michigan, Ann Arbor MI (USA)

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Abstract

The multi-subunit vacuolar ATPase pump uses ATP hydrolysis to move protons into membrane bound compartments. The pump is involved in a variety of cellular functions, including regulation of cytosolic pH, vesicular transport, endocytosis, secretion, and apoptosis. Here, we describe the cDNA cloning and chromosomal mapping of subunit D of murine V-ATPase. The mouse gene, designated Atp6m, maps to Chromosome 12, in a region of high homology with human chromosome 14q24. Evolutionary analysis of subunit D orthologs in a variety of other species reveals that this is a highly conserved protein that has been under remarkably strong negative selection during evolution, most likely reflecting its critical role in multiple cellular processes.    



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