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Vol. 91, No. 1, 2002
Issue release date: May 2002
Nephron 2002;91:148–155

Using Tandem Scanning Confocal Microscopy to Predict the Status of Donor Kidneys

Andrews P.M. · Khirabadi B.S. · Bengs B.C.
aDepartment of Cell Biology, Georgetown University Medical Center, Washington, D.C. and bAmerican Red Cross, Rockville, Md., USA

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Tandem scanning confocal microscopy (TSCM) is a noninvasive form of vital microscopy that can be used to evaluate superficial uriniferous tubules in living kidneys. Because TSCM has a number of advantages over conventional microscopic examination of renal biopsies, the present study was undertaken to determine whether the histopathological images obtained by TSCM correlate with post-transplant renal function. The kidneys of New Zealand male rabbits were harvested, flushed with Euro-Collins solution, and stored at 0–2°C for periods of 24, 48, 67 and 72 h prior to transplantation. TSCM observation of the kidneys prior to their transplantation revealed characteristic histopathological changes of the superficial proximal convoluted tubules that correlated closely with subsequent post-transplant renal function. These observations indicate that TSCM may be of significant value in evaluating the status of donor kidneys prior to their transplantation.

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