Vol. 45, No. 2, 2002
Issue release date: March–April 2002 (July 2002)
Intervirology 2002;45:101–104
(DOI:10.1159/000063231)
Short Communication
Add to my selection

Genotyping of Hepatitis E Virus from Vietnam

Hijikata M.a · Hayashi S.b · Trinh N.T.e · Ha L.D.e · Ohara H.c · Shimizu Y.K.a · Keicho N.a · Yoshikura H.d
aDepartment of Respiratory Diseases, Research Institute, bDepartment of Gastroenterology, cBureau of International Cooperation, International Medical Center of Japan, dNational Institute of Infectious Diseases, Tokyo, Japan; eThe Institute for Clinical Research in Tropical Medicine, Bach Mai Hospital, Hanoi, Vietnam
email Corresponding Author


 goto top of outline Key Words

  • Hepatitis E virus
  • Genotype
  • Vietnam

 goto top of outline Abstract

To identify the genotype of Vietnamese isolates of human hepatitis E virus (HEV), phylogenetic analysis was performed for the open reading frame (ORF) 1 and ORF2 nucleotide sequences of the viral genome. HEV was detected by RT-PCR in 9 out of 141 sera collected from patients with a diagnosis of acute sporadic hepatitis in Hanoi, Vietnam. All of them had sequences related most closely to genotype 4. In addition, the Vietnamese isolate had a single nucleotide insertion in the ORF 3 region, a characteristic reported for genotype 4 with the possible change of initiation of ORF 3 and ORF 2.

Copyright © 2002 S. Karger AG, Basel


 goto top of outline References
  1. Tam AW, Smith MM, Guerra ME, Huang CC, Bradley DW, Fry KE, Reyes GR: Hepatitis E virus (HEV): Molecular cloning and sequencing of the full-length viral genome. Virology 1991;185:120–131.
  2. Schlauder GG, Mushahwar IK: Genetic heterogeneity of hepatitis E virus. J Med Virol 2001;65:282–292.
  3. Huang CC, Nguyen D, Fernandez J, Yun KY, Fry KE, Bradley DW, Tam AW, Reyes GR: Molecular cloning and sequencing of the Mexico isolate of hepatitis E virus (HEV). Virology 1992;191:550–558.
  4. Meng XJ, Purcell RH, Halbur PG, Lehman JR, Webb DM, Tsareva TS, Haynes JS, Thacker BJ, Emerson SU: A novel virus in swine is closely related to the human hepatitis E virus. Proc Natl Acad Sci USA 1997;94:9860–9865.
  5. Schlauder GG, Dawson GJ, Erker JC, Kwo PY, Knigge MF, Smalley DL, Rosenblatt JE, Desai SM, Mushahwar IK: The sequence and phylogenetic analysis of a novel hepatitis E virus isolated from a patient with acute hepatitis reported in the United States. J Gen Virol 1998;79:447–456.
  6. Wang Y, Ling R, Erker JC, Zhang H, Li H, Desai S, Mushahwar IK, Harrison TJ: A divergent genotype of hepatitis E virus in Chinese patients with acute hepatitis. J Gen Virol 1999;80:169–177.
  7. Wang Y, Zhang H, Ling R, Li H, Harrison TJ: The complete sequence of hepatitis E virus genotype 4 reveals an alternative strategy for translation of open reading frames 2 and 3. J Gen Virol 2000;81:1675–1686.
  8. Takahashi K, Iwata K, Watanabe N, Hatahara T, Ohta Y, Baba K, Mishiro S: Full-genome nucleotide sequence of a hepatitis E virus strain that may be indigenous to Japan. Virology 2001;287:9–12.

    External Resources

  9. Hsieh SY, Yang PY, Ho YP, Chu CM, Liaw YF: Identification of a novel strain of hepatitis E virus responsible for sporadic acute hepatitis in Taiwan. J Med Virol 1998;55:300–304.

 goto top of outline Author Contacts

Minako Hijikata, MD
Department of Respiratory Diseases
Research Institute, International Medical Center of Japan
1-21-1 Toyama, Shinjuku-ku, Tokyo 162-8655 (Japan)
Tel. +81 3 3202 7181 (ext. 2875), Fax +81 3 3207 1038, E-Mail minakoh@ri.imcj.go.jp


 goto top of outline Article Information

Received: Received: December 17, 2001
Accepted after revision: January 21, 2002
Number of Print Pages : 4
Number of Figures : 2, Number of Tables : 0, Number of References : 9


 goto top of outline Publication Details

Intervirology (International Journal of Basic and Medical Virology)
Founded 1973 by J.L. Melnick; continued by F. Rapp (1986–1990); M.J. Buchmeier and C.R. Howard (1991–1993)

Vol. 45, No. 2, Year 2002 (Cover Date: March-April 2002 (Released July 2002))

Journal Editor: Rüdiger W. Braun, Stuttgart
ISSN: 0300–5526 (print), 1423–0100 (Online)

For additional information: http://www.karger.com/journals/int


Copyright / Drug Dosage / Disclaimer

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher or, in the case of photocopying, direct payment of a specified fee to the Copyright Clearance Center.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in goverment regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.